Electrochemical immunosensor signaling by employing enzyme-tagged antibody for the determination of antigen or antibody under single competition reaction format

Jun Hwang Lee; Sang Sik Yang; Byung Woo Kim; Sang Jun Sim; Heeyeop Chae; Hyun C. Yoon

doi:10.1016/j.colsurfa.2007.04.143

Electrochemical immunosensor signaling by employing enzyme-tagged antibody for the determination of antigen or antibody under single competition reaction format

Jun Hwang Lee, Sang Sik Yang, Byung Woo Kim, Sang Jun Sim, Heeyeop Chae, Hyun C. Yoon

Research output: Contribution to journal › Article › peer-review

10 Citations (Scopus)

Abstract

Target biomolecules in immunosensing, either antigens or antibodies, were detected using competitive reaction with enzyme-tagged antibody. The enzyme-tagged antibody was prepared through covalent conjugation reaction between antibody and glucose oxidase (GOX), as an electrochemical signaling molecule. By adopting dinitrophenyl (DNP) group as a target, the conjugation reaction of GOX-tagged anti-DNP antibody was performed and confirmed by liquid chromatography. The detectable concentration range of GOX-tagged anti-DNP antibody (from 0.1 μg/ml to 0.1 mg/ml) was registered by using electrochemical signaling under competitive reaction. Target biomolecules, including DNP antigen and anti-DNP antibody, were detected by using competitive immuno-reaction with the GOX-tagged anti-DNP antibody on the antigen (DNP)-functionalized sensing surface. Sensing interface was prepared by using dendrimer-assisted self-assembled monolayer. From immunosensor measurements under competition reaction format, target antigen and antibody exhibited linear detection ranges of 200 nM-2 mM and 1 μg/ml-0.1 mg/ml, respectively.

Original language	English
Pages (from-to)	509-514
Number of pages	6
Journal	Colloids and Surfaces A: Physicochemical and Engineering Aspects
Volume	313-314
DOIs	https://doi.org/10.1016/j.colsurfa.2007.04.143
Publication status	Published - 2008 Feb 1
Externally published	Yes

Keywords

Biosensor
GOX-tagged antibody
Immunosensor
Mixed monolayer

ASJC Scopus subject areas

Surfaces and Interfaces
Physical and Theoretical Chemistry
Colloid and Surface Chemistry

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10.1016/j.colsurfa.2007.04.143

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Lee, J. H., Yang, S. S., Kim, B. W., Sim, S. J., Chae, H., & Yoon, H. C. (2008). Electrochemical immunosensor signaling by employing enzyme-tagged antibody for the determination of antigen or antibody under single competition reaction format. Colloids and Surfaces A: Physicochemical and Engineering Aspects, 313-314, 509-514. https://doi.org/10.1016/j.colsurfa.2007.04.143

Electrochemical immunosensor signaling by employing enzyme-tagged antibody for the determination of antigen or antibody under single competition reaction format. / Lee, Jun Hwang; Yang, Sang Sik; Kim, Byung Woo; Sim, Sang Jun; Chae, Heeyeop; Yoon, Hyun C.

In: Colloids and Surfaces A: Physicochemical and Engineering Aspects, Vol. 313-314, 01.02.2008, p. 509-514.

Research output: Contribution to journal › Article › peer-review

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title = "Electrochemical immunosensor signaling by employing enzyme-tagged antibody for the determination of antigen or antibody under single competition reaction format",

abstract = "Target biomolecules in immunosensing, either antigens or antibodies, were detected using competitive reaction with enzyme-tagged antibody. The enzyme-tagged antibody was prepared through covalent conjugation reaction between antibody and glucose oxidase (GOX), as an electrochemical signaling molecule. By adopting dinitrophenyl (DNP) group as a target, the conjugation reaction of GOX-tagged anti-DNP antibody was performed and confirmed by liquid chromatography. The detectable concentration range of GOX-tagged anti-DNP antibody (from 0.1 μg/ml to 0.1 mg/ml) was registered by using electrochemical signaling under competitive reaction. Target biomolecules, including DNP antigen and anti-DNP antibody, were detected by using competitive immuno-reaction with the GOX-tagged anti-DNP antibody on the antigen (DNP)-functionalized sensing surface. Sensing interface was prepared by using dendrimer-assisted self-assembled monolayer. From immunosensor measurements under competition reaction format, target antigen and antibody exhibited linear detection ranges of 200 nM-2 mM and 1 μg/ml-0.1 mg/ml, respectively.",

keywords = "Biosensor, GOX-tagged antibody, Immunosensor, Mixed monolayer",

author = "Lee, {Jun Hwang} and Yang, {Sang Sik} and Kim, {Byung Woo} and Sim, {Sang Jun} and Heeyeop Chae and Yoon, {Hyun C.}",

note = "Funding Information: This research was supported by grants from the Basic Research Program of KOSEF (R01-2006-000-10240-0) and the ERC for Advanced Bioseparation Technology.",

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AU - Sim, Sang Jun

AU - Chae, Heeyeop

AU - Yoon, Hyun C.

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N2 - Target biomolecules in immunosensing, either antigens or antibodies, were detected using competitive reaction with enzyme-tagged antibody. The enzyme-tagged antibody was prepared through covalent conjugation reaction between antibody and glucose oxidase (GOX), as an electrochemical signaling molecule. By adopting dinitrophenyl (DNP) group as a target, the conjugation reaction of GOX-tagged anti-DNP antibody was performed and confirmed by liquid chromatography. The detectable concentration range of GOX-tagged anti-DNP antibody (from 0.1 μg/ml to 0.1 mg/ml) was registered by using electrochemical signaling under competitive reaction. Target biomolecules, including DNP antigen and anti-DNP antibody, were detected by using competitive immuno-reaction with the GOX-tagged anti-DNP antibody on the antigen (DNP)-functionalized sensing surface. Sensing interface was prepared by using dendrimer-assisted self-assembled monolayer. From immunosensor measurements under competition reaction format, target antigen and antibody exhibited linear detection ranges of 200 nM-2 mM and 1 μg/ml-0.1 mg/ml, respectively.

AB - Target biomolecules in immunosensing, either antigens or antibodies, were detected using competitive reaction with enzyme-tagged antibody. The enzyme-tagged antibody was prepared through covalent conjugation reaction between antibody and glucose oxidase (GOX), as an electrochemical signaling molecule. By adopting dinitrophenyl (DNP) group as a target, the conjugation reaction of GOX-tagged anti-DNP antibody was performed and confirmed by liquid chromatography. The detectable concentration range of GOX-tagged anti-DNP antibody (from 0.1 μg/ml to 0.1 mg/ml) was registered by using electrochemical signaling under competitive reaction. Target biomolecules, including DNP antigen and anti-DNP antibody, were detected by using competitive immuno-reaction with the GOX-tagged anti-DNP antibody on the antigen (DNP)-functionalized sensing surface. Sensing interface was prepared by using dendrimer-assisted self-assembled monolayer. From immunosensor measurements under competition reaction format, target antigen and antibody exhibited linear detection ranges of 200 nM-2 mM and 1 μg/ml-0.1 mg/ml, respectively.

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Electrochemical immunosensor signaling by employing enzyme-tagged antibody for the determination of antigen or antibody under single competition reaction format

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