Abstract
Target biomolecules in immunosensing, either antigens or antibodies, were detected using competitive reaction with enzyme-tagged antibody. The enzyme-tagged antibody was prepared through covalent conjugation reaction between antibody and glucose oxidase (GOX), as an electrochemical signaling molecule. By adopting dinitrophenyl (DNP) group as a target, the conjugation reaction of GOX-tagged anti-DNP antibody was performed and confirmed by liquid chromatography. The detectable concentration range of GOX-tagged anti-DNP antibody (from 0.1 μg/ml to 0.1 mg/ml) was registered by using electrochemical signaling under competitive reaction. Target biomolecules, including DNP antigen and anti-DNP antibody, were detected by using competitive immuno-reaction with the GOX-tagged anti-DNP antibody on the antigen (DNP)-functionalized sensing surface. Sensing interface was prepared by using dendrimer-assisted self-assembled monolayer. From immunosensor measurements under competition reaction format, target antigen and antibody exhibited linear detection ranges of 200 nM-2 mM and 1 μg/ml-0.1 mg/ml, respectively.
Original language | English |
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Pages (from-to) | 509-514 |
Number of pages | 6 |
Journal | Colloids and Surfaces A: Physicochemical and Engineering Aspects |
Volume | 313-314 |
DOIs | |
Publication status | Published - 2008 Feb 1 |
Externally published | Yes |
Keywords
- Biosensor
- GOX-tagged antibody
- Immunosensor
- Mixed monolayer
ASJC Scopus subject areas
- Surfaces and Interfaces
- Physical and Theoretical Chemistry
- Colloid and Surface Chemistry