Abstract
Target biomolecules in immunosensing, either antigens or antibodies, were detected using competitive reaction with enzyme-tagged antibody. The enzyme-tagged antibody was prepared through covalent conjugation reaction between antibody and glucose oxidase (GOX), as an electrochemical signaling molecule. By adopting dinitrophenyl (DNP) group as a target, the conjugation reaction of GOX-tagged anti-DNP antibody was performed and confirmed by liquid chromatography. The detectable concentration range of GOX-tagged anti-DNP antibody (from 0.1 μg/ml to 0.1 mg/ml) was registered by using electrochemical signaling under competitive reaction. Target biomolecules, including DNP antigen and anti-DNP antibody, were detected by using competitive immuno-reaction with the GOX-tagged anti-DNP antibody on the antigen (DNP)-functionalized sensing surface. Sensing interface was prepared by using dendrimer-assisted self-assembled monolayer. From immunosensor measurements under competition reaction format, target antigen and antibody exhibited linear detection ranges of 200 nM-2 mM and 1 μg/ml-0.1 mg/ml, respectively.
| Original language | English |
|---|---|
| Pages (from-to) | 509-514 |
| Number of pages | 6 |
| Journal | Colloids and Surfaces A: Physicochemical and Engineering Aspects |
| Volume | 313-314 |
| DOIs | |
| Publication status | Published - 2008 Feb 1 |
| Externally published | Yes |
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Keywords
- Biosensor
- GOX-tagged antibody
- Immunosensor
- Mixed monolayer
ASJC Scopus subject areas
- Colloid and Surface Chemistry
- Physical and Theoretical Chemistry
Cite this
Electrochemical immunosensor signaling by employing enzyme-tagged antibody for the determination of antigen or antibody under single competition reaction format. / Lee, Jun Hwang; Yang, Sang Sik; Kim, Byung Woo; Sim, Sang Jun; Chae, Heeyeop; Yoon, Hyun C.
In: Colloids and Surfaces A: Physicochemical and Engineering Aspects, Vol. 313-314, 01.02.2008, p. 509-514.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Electrochemical immunosensor signaling by employing enzyme-tagged antibody for the determination of antigen or antibody under single competition reaction format
AU - Lee, Jun Hwang
AU - Yang, Sang Sik
AU - Kim, Byung Woo
AU - Sim, Sang Jun
AU - Chae, Heeyeop
AU - Yoon, Hyun C.
PY - 2008/2/1
Y1 - 2008/2/1
N2 - Target biomolecules in immunosensing, either antigens or antibodies, were detected using competitive reaction with enzyme-tagged antibody. The enzyme-tagged antibody was prepared through covalent conjugation reaction between antibody and glucose oxidase (GOX), as an electrochemical signaling molecule. By adopting dinitrophenyl (DNP) group as a target, the conjugation reaction of GOX-tagged anti-DNP antibody was performed and confirmed by liquid chromatography. The detectable concentration range of GOX-tagged anti-DNP antibody (from 0.1 μg/ml to 0.1 mg/ml) was registered by using electrochemical signaling under competitive reaction. Target biomolecules, including DNP antigen and anti-DNP antibody, were detected by using competitive immuno-reaction with the GOX-tagged anti-DNP antibody on the antigen (DNP)-functionalized sensing surface. Sensing interface was prepared by using dendrimer-assisted self-assembled monolayer. From immunosensor measurements under competition reaction format, target antigen and antibody exhibited linear detection ranges of 200 nM-2 mM and 1 μg/ml-0.1 mg/ml, respectively.
AB - Target biomolecules in immunosensing, either antigens or antibodies, were detected using competitive reaction with enzyme-tagged antibody. The enzyme-tagged antibody was prepared through covalent conjugation reaction between antibody and glucose oxidase (GOX), as an electrochemical signaling molecule. By adopting dinitrophenyl (DNP) group as a target, the conjugation reaction of GOX-tagged anti-DNP antibody was performed and confirmed by liquid chromatography. The detectable concentration range of GOX-tagged anti-DNP antibody (from 0.1 μg/ml to 0.1 mg/ml) was registered by using electrochemical signaling under competitive reaction. Target biomolecules, including DNP antigen and anti-DNP antibody, were detected by using competitive immuno-reaction with the GOX-tagged anti-DNP antibody on the antigen (DNP)-functionalized sensing surface. Sensing interface was prepared by using dendrimer-assisted self-assembled monolayer. From immunosensor measurements under competition reaction format, target antigen and antibody exhibited linear detection ranges of 200 nM-2 mM and 1 μg/ml-0.1 mg/ml, respectively.
KW - Biosensor
KW - GOX-tagged antibody
KW - Immunosensor
KW - Mixed monolayer
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UR - http://www.scopus.com/inward/citedby.url?scp=37349075777&partnerID=8YFLogxK
U2 - 10.1016/j.colsurfa.2007.04.143
DO - 10.1016/j.colsurfa.2007.04.143
M3 - Article
AN - SCOPUS:37349075777
VL - 313-314
SP - 509
EP - 514
JO - Colloids and Surfaces A: Physicochemical and Engineering Aspects
JF - Colloids and Surfaces A: Physicochemical and Engineering Aspects
SN - 0927-7757
ER -