Electrochemical immunosensor signaling by employing enzyme-tagged antibody for the determination of antigen or antibody under single competition reaction format

Jun Hwang Lee, Sang Sik Yang, Byung Woo Kim, Sang Jun Sim, Heeyeop Chae, Hyun C. Yoon

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Target biomolecules in immunosensing, either antigens or antibodies, were detected using competitive reaction with enzyme-tagged antibody. The enzyme-tagged antibody was prepared through covalent conjugation reaction between antibody and glucose oxidase (GOX), as an electrochemical signaling molecule. By adopting dinitrophenyl (DNP) group as a target, the conjugation reaction of GOX-tagged anti-DNP antibody was performed and confirmed by liquid chromatography. The detectable concentration range of GOX-tagged anti-DNP antibody (from 0.1 μg/ml to 0.1 mg/ml) was registered by using electrochemical signaling under competitive reaction. Target biomolecules, including DNP antigen and anti-DNP antibody, were detected by using competitive immuno-reaction with the GOX-tagged anti-DNP antibody on the antigen (DNP)-functionalized sensing surface. Sensing interface was prepared by using dendrimer-assisted self-assembled monolayer. From immunosensor measurements under competition reaction format, target antigen and antibody exhibited linear detection ranges of 200 nM-2 mM and 1 μg/ml-0.1 mg/ml, respectively.

Original languageEnglish
Pages (from-to)509-514
Number of pages6
JournalColloids and Surfaces A: Physicochemical and Engineering Aspects
Volume313-314
DOIs
Publication statusPublished - 2008 Feb 1

Keywords

  • Biosensor
  • GOX-tagged antibody
  • Immunosensor
  • Mixed monolayer

ASJC Scopus subject areas

  • Surfaces and Interfaces
  • Physical and Theoretical Chemistry
  • Colloid and Surface Chemistry

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