Abstract
A critical factor for the performance of a biofuel cell is an immobilization of the redox enzyme for continuous catalytic reaction and efficient electron transfer. However, the main obstacle associated with enzyme electrode is the reduced surface area for the accommodation of enzymes, leading to poor power output. This study aimed to optimize the efficient electrical communication for glucose oxidase (GOx) on the surface of a graphite oxide/cobalt hydroxide/chitosan composite as mediator, thereby enhancing the generation of power output. Immobilization efficiency was affected by the different concentrations of 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide (EDC)/N-hydroxysuccinimide (NHS). Also, the surface of enzyme electrode was observed by XPS, Raman, and AFM, respectively. The electrochemical characterization showed that the immobilized GOx possesses the highest activity at EDC:NHS(40:80 mM) concentration. The power output under the optimal condition was found to be 2.24 mWcm−2 of power density using the three-electrode cell in 0.1 M PBS solution at room temperature.
Original language | English |
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Pages (from-to) | 3251-3258 |
Number of pages | 8 |
Journal | International Journal of Hydrogen Energy |
Volume | 46 |
Issue number | 4 |
DOIs | |
Publication status | Published - 2021 Jan 14 |
Keywords
- Biofuel cell
- Electron transfer
- Enzyme immobilization
- Glucose oxidase
- Mediator
ASJC Scopus subject areas
- Renewable Energy, Sustainability and the Environment
- Fuel Technology
- Condensed Matter Physics
- Energy Engineering and Power Technology