Electrophysiological evidence for functional astrocytic P2X3 receptors in the mouse trigeminal caudal nucleus

Jaekwang Lee, Jin Young Bae, Changjoon Lee, Yong Chul Bae

Research output: Contribution to journalShort survey

Abstract

Recently, we reported that astrocytes in the trigeminal caudal nucleus (Vc) of the brain stem express a purinergic receptor P2X3, which is involved in the craniofacial pathologic pain. Although we observed protein expression of P2X3 receptors (P2X3 Rs) in the astrocyte of the Vc, it is still unclear that astrocyte has functional P2X3Rs in Vc. To address this issue, we recorded asrtocytic P2X3Rs by using whole cell voltage-clamp recording in the Vc of the GFAP-GFP mice, which was used as a guide to astrocytes with green fluorescence. While measuring voltage ramp-induced astrocytic membrane current, we found the amplitude of the current was increased when we applied P2-purinoreceptor agonist, a,ß-meATP. This increase was blocked by co-application of A317491, P2X3R antagonist. These results demonstrate that astrocytes in the Vc express functional P2X3Rs, which might be critical in craniofacial pathologic pain.

Original languageEnglish
Pages (from-to)88-93
Number of pages6
JournalExperimental Neurobiology
Volume27
Issue number2
DOIs
Publication statusPublished - 2018 Apr 1
Externally publishedYes

Fingerprint

Trigeminal Caudal Nucleus
Purinergic P2X3 Receptors
Astrocytes
Facial Pain
Architectural Accessibility
Brain Stem
Fluorescence
Membranes

Keywords

  • Astrocytes
  • Electrophysiology
  • Pain
  • Purinergic P2X
  • Trigeminal caudal nucleus

ASJC Scopus subject areas

  • Clinical Neurology
  • Cellular and Molecular Neuroscience

Cite this

Electrophysiological evidence for functional astrocytic P2X3 receptors in the mouse trigeminal caudal nucleus. / Lee, Jaekwang; Bae, Jin Young; Lee, Changjoon; Bae, Yong Chul.

In: Experimental Neurobiology, Vol. 27, No. 2, 01.04.2018, p. 88-93.

Research output: Contribution to journalShort survey

Lee, Jaekwang ; Bae, Jin Young ; Lee, Changjoon ; Bae, Yong Chul. / Electrophysiological evidence for functional astrocytic P2X3 receptors in the mouse trigeminal caudal nucleus. In: Experimental Neurobiology. 2018 ; Vol. 27, No. 2. pp. 88-93.
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