Electrophysiology of the suprachiasmatic nucleus: synaptic transmission, membrane properties, and neuronal synchronization.

F. E. Dudek, Yang In Kim, Y. Bouskila

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Knowledge of the neuronal membrane properties and synaptic physiology of the suprachiasmatic nucleus (SCN) is critical for an understanding of the cellular basis of circadian rhythms in mammals. The hypothalamic slice preparations from rodents and a combination of electrophysiological techniques (i.e., extracellular single- and multiple-unit recording, intracellular recording, and whole-cell patch clamp) were used to study (1) the role of excitatory and inhibitory amino acids (i.e., glutamate and gamma-aminobutyric acid [GABA] in synaptic transmission, (2) the membrane properties of SCN neurons, and (3) the mechanisms of neuronal synchronization. Antagonists for N-methyl-D-aspartate (NMDA) receptors and non-NMDA receptors blocked excitatory postsynaptic potentials (EPSPs) evoked by stimulation of the optic nerve or other sites when SCN cells were depolarized or at rest, respectively. Bicuculline blocked inhibitory postsynaptic potentials (IPSPs) that were evoked by local stimulation or that occurred spontaneously. The IPSP reversal potential was near the Cl- equilibrium potential, and was shifted to depolarized levels by raising intracellular [Cl-]. Thus, glutamate and GABA appear to mediate fast excitatory and inhibitory synaptic transmission in the SCN. Some SCN neurons, but not all of them, had low-threshold Ca2+ spikes and time-dependent inward rectification, thus indicating that the electrical properties of SCN neurons are not homogenous. Neurons with a firing rate of > 6 Hz had a regular pattern, and neurons with a rate of < 4 Hz had an irregular pattern; since both the firing rate and pattern could be modified with injected currents, SCN neurons with different firing patterns are unlikely to represent distinct classes of cells.(ABSTRACT TRUNCATED AT 250 WORDS)

Original languageEnglish
JournalJournal of Biological Rhythms
Volume8 Suppl
Publication statusPublished - 1993 Dec 1
Externally publishedYes

Fingerprint

Synaptic Membranes
electrophysiology
synaptic transmission
Suprachiasmatic Nucleus
Electrophysiology
Synaptic Transmission
neurons
Neurons
gamma-aminobutyric acid
Inhibitory Postsynaptic Potentials
aspartic acid
glutamates
gamma-Aminobutyric Acid
Glutamic Acid
electrical properties
receptors
evoked potentials
D-Aspartic Acid
cell nucleus
Excitatory Amino Acids

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Physiology
  • Physiology (medical)

Cite this

Electrophysiology of the suprachiasmatic nucleus : synaptic transmission, membrane properties, and neuronal synchronization. / Dudek, F. E.; Kim, Yang In; Bouskila, Y.

In: Journal of Biological Rhythms, Vol. 8 Suppl, 01.12.1993.

Research output: Contribution to journalArticle

@article{71f313e8e9264b4a96e923a4c3f508d7,
title = "Electrophysiology of the suprachiasmatic nucleus: synaptic transmission, membrane properties, and neuronal synchronization.",
abstract = "Knowledge of the neuronal membrane properties and synaptic physiology of the suprachiasmatic nucleus (SCN) is critical for an understanding of the cellular basis of circadian rhythms in mammals. The hypothalamic slice preparations from rodents and a combination of electrophysiological techniques (i.e., extracellular single- and multiple-unit recording, intracellular recording, and whole-cell patch clamp) were used to study (1) the role of excitatory and inhibitory amino acids (i.e., glutamate and gamma-aminobutyric acid [GABA] in synaptic transmission, (2) the membrane properties of SCN neurons, and (3) the mechanisms of neuronal synchronization. Antagonists for N-methyl-D-aspartate (NMDA) receptors and non-NMDA receptors blocked excitatory postsynaptic potentials (EPSPs) evoked by stimulation of the optic nerve or other sites when SCN cells were depolarized or at rest, respectively. Bicuculline blocked inhibitory postsynaptic potentials (IPSPs) that were evoked by local stimulation or that occurred spontaneously. The IPSP reversal potential was near the Cl- equilibrium potential, and was shifted to depolarized levels by raising intracellular [Cl-]. Thus, glutamate and GABA appear to mediate fast excitatory and inhibitory synaptic transmission in the SCN. Some SCN neurons, but not all of them, had low-threshold Ca2+ spikes and time-dependent inward rectification, thus indicating that the electrical properties of SCN neurons are not homogenous. Neurons with a firing rate of > 6 Hz had a regular pattern, and neurons with a rate of < 4 Hz had an irregular pattern; since both the firing rate and pattern could be modified with injected currents, SCN neurons with different firing patterns are unlikely to represent distinct classes of cells.(ABSTRACT TRUNCATED AT 250 WORDS)",
author = "Dudek, {F. E.} and Kim, {Yang In} and Y. Bouskila",
year = "1993",
month = "12",
day = "1",
language = "English",
volume = "8 Suppl",
journal = "Journal of Biological Rhythms",
issn = "0748-7304",
publisher = "SAGE Publications Inc.",

}

TY - JOUR

T1 - Electrophysiology of the suprachiasmatic nucleus

T2 - synaptic transmission, membrane properties, and neuronal synchronization.

AU - Dudek, F. E.

AU - Kim, Yang In

AU - Bouskila, Y.

PY - 1993/12/1

Y1 - 1993/12/1

N2 - Knowledge of the neuronal membrane properties and synaptic physiology of the suprachiasmatic nucleus (SCN) is critical for an understanding of the cellular basis of circadian rhythms in mammals. The hypothalamic slice preparations from rodents and a combination of electrophysiological techniques (i.e., extracellular single- and multiple-unit recording, intracellular recording, and whole-cell patch clamp) were used to study (1) the role of excitatory and inhibitory amino acids (i.e., glutamate and gamma-aminobutyric acid [GABA] in synaptic transmission, (2) the membrane properties of SCN neurons, and (3) the mechanisms of neuronal synchronization. Antagonists for N-methyl-D-aspartate (NMDA) receptors and non-NMDA receptors blocked excitatory postsynaptic potentials (EPSPs) evoked by stimulation of the optic nerve or other sites when SCN cells were depolarized or at rest, respectively. Bicuculline blocked inhibitory postsynaptic potentials (IPSPs) that were evoked by local stimulation or that occurred spontaneously. The IPSP reversal potential was near the Cl- equilibrium potential, and was shifted to depolarized levels by raising intracellular [Cl-]. Thus, glutamate and GABA appear to mediate fast excitatory and inhibitory synaptic transmission in the SCN. Some SCN neurons, but not all of them, had low-threshold Ca2+ spikes and time-dependent inward rectification, thus indicating that the electrical properties of SCN neurons are not homogenous. Neurons with a firing rate of > 6 Hz had a regular pattern, and neurons with a rate of < 4 Hz had an irregular pattern; since both the firing rate and pattern could be modified with injected currents, SCN neurons with different firing patterns are unlikely to represent distinct classes of cells.(ABSTRACT TRUNCATED AT 250 WORDS)

AB - Knowledge of the neuronal membrane properties and synaptic physiology of the suprachiasmatic nucleus (SCN) is critical for an understanding of the cellular basis of circadian rhythms in mammals. The hypothalamic slice preparations from rodents and a combination of electrophysiological techniques (i.e., extracellular single- and multiple-unit recording, intracellular recording, and whole-cell patch clamp) were used to study (1) the role of excitatory and inhibitory amino acids (i.e., glutamate and gamma-aminobutyric acid [GABA] in synaptic transmission, (2) the membrane properties of SCN neurons, and (3) the mechanisms of neuronal synchronization. Antagonists for N-methyl-D-aspartate (NMDA) receptors and non-NMDA receptors blocked excitatory postsynaptic potentials (EPSPs) evoked by stimulation of the optic nerve or other sites when SCN cells were depolarized or at rest, respectively. Bicuculline blocked inhibitory postsynaptic potentials (IPSPs) that were evoked by local stimulation or that occurred spontaneously. The IPSP reversal potential was near the Cl- equilibrium potential, and was shifted to depolarized levels by raising intracellular [Cl-]. Thus, glutamate and GABA appear to mediate fast excitatory and inhibitory synaptic transmission in the SCN. Some SCN neurons, but not all of them, had low-threshold Ca2+ spikes and time-dependent inward rectification, thus indicating that the electrical properties of SCN neurons are not homogenous. Neurons with a firing rate of > 6 Hz had a regular pattern, and neurons with a rate of < 4 Hz had an irregular pattern; since both the firing rate and pattern could be modified with injected currents, SCN neurons with different firing patterns are unlikely to represent distinct classes of cells.(ABSTRACT TRUNCATED AT 250 WORDS)

UR - http://www.scopus.com/inward/record.url?scp=0027769206&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0027769206&partnerID=8YFLogxK

M3 - Article

C2 - 8274761

AN - SCOPUS:0027769206

VL - 8 Suppl

JO - Journal of Biological Rhythms

JF - Journal of Biological Rhythms

SN - 0748-7304

ER -