Abstract
Ion-permselective nanochannel-based sample preconcentration or electropreconcentration has been demonstrated as an effective technique for concentrating charged analytes at the interface between a micro- and nanochannel. The anion-selective electropreconcentration involves extraction of hydroxide in the preconcentrated sample plug, resulting in pH decrease. We investigated the pH change in a microchannel using charged pH indicators with different conditions including running buffer pH, sample channel electric field, and salt concentration. The anion-selective preconcentration showed pH decrease from 11 to under 7 in the preconcentrated sample plug. Therefore, careful design and interpretation are required with pH-dependent experiments such as analyzing enzyme or antibody characteristics. The pH change could be mitigated by reducing the sample channel electric field and/or increasing salt concentration in the buffer.
| Original language | English |
|---|---|
| Journal | Electrophoresis |
| DOIs | |
| Publication status | Accepted/In press - 2017 Jan 1 |
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Keywords
- Electropreconcentration
- Local pH change
- Sample preconcentration
ASJC Scopus subject areas
- Biochemistry
- Clinical Biochemistry
Cite this
Electropreconcentration-induced local pH change. / Chun, Honggu.
In: Electrophoresis, 01.01.2017.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Electropreconcentration-induced local pH change
AU - Chun, Honggu
PY - 2017/1/1
Y1 - 2017/1/1
N2 - Ion-permselective nanochannel-based sample preconcentration or electropreconcentration has been demonstrated as an effective technique for concentrating charged analytes at the interface between a micro- and nanochannel. The anion-selective electropreconcentration involves extraction of hydroxide in the preconcentrated sample plug, resulting in pH decrease. We investigated the pH change in a microchannel using charged pH indicators with different conditions including running buffer pH, sample channel electric field, and salt concentration. The anion-selective preconcentration showed pH decrease from 11 to under 7 in the preconcentrated sample plug. Therefore, careful design and interpretation are required with pH-dependent experiments such as analyzing enzyme or antibody characteristics. The pH change could be mitigated by reducing the sample channel electric field and/or increasing salt concentration in the buffer.
AB - Ion-permselective nanochannel-based sample preconcentration or electropreconcentration has been demonstrated as an effective technique for concentrating charged analytes at the interface between a micro- and nanochannel. The anion-selective electropreconcentration involves extraction of hydroxide in the preconcentrated sample plug, resulting in pH decrease. We investigated the pH change in a microchannel using charged pH indicators with different conditions including running buffer pH, sample channel electric field, and salt concentration. The anion-selective preconcentration showed pH decrease from 11 to under 7 in the preconcentrated sample plug. Therefore, careful design and interpretation are required with pH-dependent experiments such as analyzing enzyme or antibody characteristics. The pH change could be mitigated by reducing the sample channel electric field and/or increasing salt concentration in the buffer.
KW - Electropreconcentration
KW - Local pH change
KW - Sample preconcentration
UR - http://www.scopus.com/inward/record.url?scp=85034957553&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85034957553&partnerID=8YFLogxK
U2 - 10.1002/elps.201700373
DO - 10.1002/elps.201700373
M3 - Article
C2 - 29077205
AN - SCOPUS:85034957553
JO - Electrophoresis
JF - Electrophoresis
SN - 0173-0835
ER -