Electrostatic interaction effect for human DNA separation with functionalized mesoporous silicas

Hong Kyung Choi, Jeong Ho Chang, Il Hwan Ko, Jin Hyung Lee, Bong Yong Jeong, Jong Hee Kim, Jung Bae Kim

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

This work describes the development of highly efficient human DNA separation with functionalized mesoporous silica (FMS) materials. To demonstrate the electrostatic interaction effect between the target DNA molecules and FMS, three aminofunctionality types comprised of a mono-, a di-, and a tri-amine functional group were introduced on the inner surfaces of mesoporous silica particles. Systematic characterization of the synthesized materials was achieved by solid-state 29Si and 13C-NMR techniques, BET, FT-IR, and XPS. The DNA separation efficiency was explored via the function of the amino-group number, the amount used, and the added NaCl concentration. The DNA adsorption yields were high in terms of the use of triaminofunctionalized FMS at the 10 ng/L level, and the DNA desorption efficiency showed the optimum level at over 3.0 M NaCl concentration. The use of FMS in a DNA separation process provides numerous advantages over the conventional silica-based process.

Original languageEnglish
Pages (from-to)805-810
Number of pages6
JournalJournal of Solid State Chemistry
Volume184
Issue number4
DOIs
Publication statusPublished - 2011 Apr

Keywords

  • Confocal microscope
  • DNA separation
  • Electrostatic interaction
  • Functionalized mesoporous silicas
  • Solid-state NMR

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Ceramics and Composites
  • Condensed Matter Physics
  • Physical and Theoretical Chemistry
  • Inorganic Chemistry
  • Materials Chemistry

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