Elevated and biallelic expression of p73 is associated with progression of human bladder cancer

Sung-Gil Chi, Sung Goo Chang, Sun Ju Lee, Choong Hyun Lee, J. Il Kim, Jae Hoon Park

Research output: Contribution to journalArticle

70 Citations (Scopus)

Abstract

p73, a member of the p53 family at 1p36.3, has been demonstrated to be expressed monoallelically and induce apoptosis or G1 arrest of the cell cycle. To explore the candidacy of p73 as a suppressor in bladder tumorigenesis, we examined expression level, allelic origin, and mutation of p73 mRNA in 45 primary bladder carcinomas. Quantitative PCR analysis showed no allelic loss of the gene but showed various levels of mRNA expression in both carcinoma and noncancerous tissues. Elevated expression of p73 was frequently observed in carcinoma tissues [18 (40.0%) of 45] and showed a strong correlation with tumor stage or grade. Allotyping analysis using a StyI polymorphism detected biallelic expression in 12 (52.2%) of 23 heterozygous carcinomas but none in 4 noncancerous tissues. Tumor-specific biallelic expression was also identified from one matched set. In addition, 8 (66.7 %) of these 12 expressed high levels of p73 mRNA, whereas only 2 (18.2%) of 11 monoallelic expressors showed high expression, which suggests that the increased expression of p73 might be caused by the transcriptional activation of a silent allele in carcinomas. Single-strand conformational polymorphism analysis of the entire coding region of p73 revealed no mutation, whereas 12 (26.7%) of the same set showed p53 alterations. No relationship between expression of p73 and p53 mutation or expression of p21(Waf1) or MDM2 was identified. Taken together, our data argue that p73 does not play a role as a tumor suppressor in bladder carcinogenesis and suggest that the activation of a silent allele may contribute to the progression of bladder tumors.

Original languageEnglish
Pages (from-to)2791-2793
Number of pages3
JournalCancer Research
Volume59
Issue number12
Publication statusPublished - 1999 Jun 15
Externally publishedYes

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Urinary Bladder Neoplasms
Carcinoma
Urinary Bladder
Messenger RNA
Mutation
Carcinogenesis
Alleles
G1 Phase Cell Cycle Checkpoints
Neoplasms
Loss of Heterozygosity
Transcriptional Activation
Apoptosis
Polymerase Chain Reaction
Genes

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Chi, S-G., Chang, S. G., Lee, S. J., Lee, C. H., Kim, J. I., & Park, J. H. (1999). Elevated and biallelic expression of p73 is associated with progression of human bladder cancer. Cancer Research, 59(12), 2791-2793.

Elevated and biallelic expression of p73 is associated with progression of human bladder cancer. / Chi, Sung-Gil; Chang, Sung Goo; Lee, Sun Ju; Lee, Choong Hyun; Kim, J. Il; Park, Jae Hoon.

In: Cancer Research, Vol. 59, No. 12, 15.06.1999, p. 2791-2793.

Research output: Contribution to journalArticle

Chi, S-G, Chang, SG, Lee, SJ, Lee, CH, Kim, JI & Park, JH 1999, 'Elevated and biallelic expression of p73 is associated with progression of human bladder cancer', Cancer Research, vol. 59, no. 12, pp. 2791-2793.
Chi S-G, Chang SG, Lee SJ, Lee CH, Kim JI, Park JH. Elevated and biallelic expression of p73 is associated with progression of human bladder cancer. Cancer Research. 1999 Jun 15;59(12):2791-2793.
Chi, Sung-Gil ; Chang, Sung Goo ; Lee, Sun Ju ; Lee, Choong Hyun ; Kim, J. Il ; Park, Jae Hoon. / Elevated and biallelic expression of p73 is associated with progression of human bladder cancer. In: Cancer Research. 1999 ; Vol. 59, No. 12. pp. 2791-2793.
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