Elongation factor P controls translation of the mgtA gene encoding a Mg 2+ transporter during Salmonella infection

Eunna Choi, Daesil Nam, Jeongjoon Choi, Shinae Park, Jung Shin Lee, Eun-Jin Lee

Research output: Contribution to journalArticle


Ribosome often stalls on mRNA sequences harboring consecutive proline codons. Elongation factor P (EF-P) is required for the stalled ribosome to continue translation and thus the absence of EF-P affects translation of the associated open reading frame. Here we report that EF-P controls translation of the mgtA gene encoding a Mg 2+ -transporting ATPase from the intracellualr pathogen Salmonella enterica serovar Typhimurium. EF-P's effect on mgtA translation is dependent on the 550th and 551st proline codons in the coding region and thus substitution of those proline codons eliminates EF-P-mediated control of MgtA protein without affecting the Mg 2+ -transporting activity of the mgtA gene. The Pro550 and Pro551-substituted mgtA gene promotes Salmonella's intramacrophage survival and mouse virulence, suggesting that EF-P-mediated translational control of the mgtA gene is required for Salmonella pathogenesis.

Original languageEnglish
Article numbere00680
Issue number4
Publication statusPublished - 2019 Apr 1
Externally publishedYes



  • elongation factor P
  • Mg transporter
  • mgtA
  • translational control

ASJC Scopus subject areas

  • Microbiology

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