Elongation factor P controls translation of the mgtA gene encoding a Mg 2+ transporter during Salmonella infection

Eunna Choi, Daesil Nam, Jeongjoon Choi, Shinae Park, Jung Shin Lee, Eun-Jin Lee

Research output: Contribution to journalArticle

Abstract

Ribosome often stalls on mRNA sequences harboring consecutive proline codons. Elongation factor P (EF-P) is required for the stalled ribosome to continue translation and thus the absence of EF-P affects translation of the associated open reading frame. Here we report that EF-P controls translation of the mgtA gene encoding a Mg 2+ -transporting ATPase from the intracellualr pathogen Salmonella enterica serovar Typhimurium. EF-P's effect on mgtA translation is dependent on the 550th and 551st proline codons in the coding region and thus substitution of those proline codons eliminates EF-P-mediated control of MgtA protein without affecting the Mg 2+ -transporting activity of the mgtA gene. The Pro550 and Pro551-substituted mgtA gene promotes Salmonella's intramacrophage survival and mouse virulence, suggesting that EF-P-mediated translational control of the mgtA gene is required for Salmonella pathogenesis.

Original languageEnglish
Article numbere00680
JournalMicrobiologyOpen
Volume8
Issue number4
DOIs
Publication statusPublished - 2019 Apr 1
Externally publishedYes

Fingerprint

Salmonella Infections
Proline
Codon
Genes
Ribosomes
Salmonella
Salmonella enterica
Open Reading Frames
Virulence
Adenosine Triphosphatases
factor EF-P
Messenger RNA
Proteins

Keywords

  • elongation factor P
  • Mg transporter
  • mgtA
  • translational control

ASJC Scopus subject areas

  • Microbiology

Cite this

Elongation factor P controls translation of the mgtA gene encoding a Mg 2+ transporter during Salmonella infection . / Choi, Eunna; Nam, Daesil; Choi, Jeongjoon; Park, Shinae; Lee, Jung Shin; Lee, Eun-Jin.

In: MicrobiologyOpen, Vol. 8, No. 4, e00680, 01.04.2019.

Research output: Contribution to journalArticle

Choi, Eunna ; Nam, Daesil ; Choi, Jeongjoon ; Park, Shinae ; Lee, Jung Shin ; Lee, Eun-Jin. / Elongation factor P controls translation of the mgtA gene encoding a Mg 2+ transporter during Salmonella infection In: MicrobiologyOpen. 2019 ; Vol. 8, No. 4.
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