Elucidation of ethanol tolerance mechanisms in Saccharomyces cerevisiae by global metabolite profiling

Sooah Kim, Jungyeon Kim, Ju Hwan Song, Young Hoon Jung, Il Sup Choi, Wonja Choi, Yong Cheol Park, Jin Ho Seo, Kyoung Heon Kim

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Ethanol, the major fermentation product of yeast, is a stress factor in yeast. We previously constructed an ethanol-tolerant mutant yeast iETS3 by using the global transcriptional machinery engineering. However, the ethanol-tolerance mechanism has not been systematically investigated. In this study, global metabolite profiling was carried out, mainly by gas chromatography/time-of-flight mass spectrometry (GC/TOF MS), to investigate the mechanisms of ethanol tolerance in iETS3. A total of 108 intracellular metabolites were identified by GC/TOF MS and high performance liquid chromatography, and these metabolites were mostly intermediates of the central carbon metabolism. The metabolite profiles of iETS3 and BY4741, cultured with or without ethanol, were significantly different based on principal component and hierarchical clustering analyses. Our metabolomic analyses identified the compositional changes in cell membranes and the activation of glutamate metabolism and the trehalose synthetic pathway as the possible mechanisms for the ethanol tolerance. These metabolic traits can be considered possible targets for further improvement of ethanol tolerance in the mutant. For example, the KGD1 deletion mutant, with up-regulated glutamate metabolism, showed increased tolerance to ethanol. This study has demonstrated that metabolomics can be a useful tool for strain improvement and phenotypic analysis of microorganisms under stress.

Original languageEnglish
Pages (from-to)1221-1229
Number of pages9
JournalBiotechnology Journal
Volume11
Issue number9
DOIs
Publication statusPublished - 2016 Sep 1

Fingerprint

Saccharomyces cerevisiae
Ethanol
Metabolomics
Yeasts
Gas Chromatography
Glutamic Acid
Mass Spectrometry
Trehalose
Fermentation
Cluster Analysis
Carbon
High Pressure Liquid Chromatography
Cell Membrane

Keywords

  • Ethanol tolerance
  • Metabolite profiling
  • Metabolomics
  • Saccharomyces cerevisiae

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Molecular Medicine

Cite this

Elucidation of ethanol tolerance mechanisms in Saccharomyces cerevisiae by global metabolite profiling. / Kim, Sooah; Kim, Jungyeon; Song, Ju Hwan; Jung, Young Hoon; Choi, Il Sup; Choi, Wonja; Park, Yong Cheol; Seo, Jin Ho; Kim, Kyoung Heon.

In: Biotechnology Journal, Vol. 11, No. 9, 01.09.2016, p. 1221-1229.

Research output: Contribution to journalArticle

Kim, S, Kim, J, Song, JH, Jung, YH, Choi, IS, Choi, W, Park, YC, Seo, JH & Kim, KH 2016, 'Elucidation of ethanol tolerance mechanisms in Saccharomyces cerevisiae by global metabolite profiling', Biotechnology Journal, vol. 11, no. 9, pp. 1221-1229. https://doi.org/10.1002/biot.201500613
Kim, Sooah ; Kim, Jungyeon ; Song, Ju Hwan ; Jung, Young Hoon ; Choi, Il Sup ; Choi, Wonja ; Park, Yong Cheol ; Seo, Jin Ho ; Kim, Kyoung Heon. / Elucidation of ethanol tolerance mechanisms in Saccharomyces cerevisiae by global metabolite profiling. In: Biotechnology Journal. 2016 ; Vol. 11, No. 9. pp. 1221-1229.
@article{83cd936e282c47b2b23671050f1ea419,
title = "Elucidation of ethanol tolerance mechanisms in Saccharomyces cerevisiae by global metabolite profiling",
abstract = "Ethanol, the major fermentation product of yeast, is a stress factor in yeast. We previously constructed an ethanol-tolerant mutant yeast iETS3 by using the global transcriptional machinery engineering. However, the ethanol-tolerance mechanism has not been systematically investigated. In this study, global metabolite profiling was carried out, mainly by gas chromatography/time-of-flight mass spectrometry (GC/TOF MS), to investigate the mechanisms of ethanol tolerance in iETS3. A total of 108 intracellular metabolites were identified by GC/TOF MS and high performance liquid chromatography, and these metabolites were mostly intermediates of the central carbon metabolism. The metabolite profiles of iETS3 and BY4741, cultured with or without ethanol, were significantly different based on principal component and hierarchical clustering analyses. Our metabolomic analyses identified the compositional changes in cell membranes and the activation of glutamate metabolism and the trehalose synthetic pathway as the possible mechanisms for the ethanol tolerance. These metabolic traits can be considered possible targets for further improvement of ethanol tolerance in the mutant. For example, the KGD1 deletion mutant, with up-regulated glutamate metabolism, showed increased tolerance to ethanol. This study has demonstrated that metabolomics can be a useful tool for strain improvement and phenotypic analysis of microorganisms under stress.",
keywords = "Ethanol tolerance, Metabolite profiling, Metabolomics, Saccharomyces cerevisiae",
author = "Sooah Kim and Jungyeon Kim and Song, {Ju Hwan} and Jung, {Young Hoon} and Choi, {Il Sup} and Wonja Choi and Park, {Yong Cheol} and Seo, {Jin Ho} and Kim, {Kyoung Heon}",
year = "2016",
month = "9",
day = "1",
doi = "10.1002/biot.201500613",
language = "English",
volume = "11",
pages = "1221--1229",
journal = "Biotechnology Journal",
issn = "1860-6768",
publisher = "Wiley-VCH Verlag",
number = "9",

}

TY - JOUR

T1 - Elucidation of ethanol tolerance mechanisms in Saccharomyces cerevisiae by global metabolite profiling

AU - Kim, Sooah

AU - Kim, Jungyeon

AU - Song, Ju Hwan

AU - Jung, Young Hoon

AU - Choi, Il Sup

AU - Choi, Wonja

AU - Park, Yong Cheol

AU - Seo, Jin Ho

AU - Kim, Kyoung Heon

PY - 2016/9/1

Y1 - 2016/9/1

N2 - Ethanol, the major fermentation product of yeast, is a stress factor in yeast. We previously constructed an ethanol-tolerant mutant yeast iETS3 by using the global transcriptional machinery engineering. However, the ethanol-tolerance mechanism has not been systematically investigated. In this study, global metabolite profiling was carried out, mainly by gas chromatography/time-of-flight mass spectrometry (GC/TOF MS), to investigate the mechanisms of ethanol tolerance in iETS3. A total of 108 intracellular metabolites were identified by GC/TOF MS and high performance liquid chromatography, and these metabolites were mostly intermediates of the central carbon metabolism. The metabolite profiles of iETS3 and BY4741, cultured with or without ethanol, were significantly different based on principal component and hierarchical clustering analyses. Our metabolomic analyses identified the compositional changes in cell membranes and the activation of glutamate metabolism and the trehalose synthetic pathway as the possible mechanisms for the ethanol tolerance. These metabolic traits can be considered possible targets for further improvement of ethanol tolerance in the mutant. For example, the KGD1 deletion mutant, with up-regulated glutamate metabolism, showed increased tolerance to ethanol. This study has demonstrated that metabolomics can be a useful tool for strain improvement and phenotypic analysis of microorganisms under stress.

AB - Ethanol, the major fermentation product of yeast, is a stress factor in yeast. We previously constructed an ethanol-tolerant mutant yeast iETS3 by using the global transcriptional machinery engineering. However, the ethanol-tolerance mechanism has not been systematically investigated. In this study, global metabolite profiling was carried out, mainly by gas chromatography/time-of-flight mass spectrometry (GC/TOF MS), to investigate the mechanisms of ethanol tolerance in iETS3. A total of 108 intracellular metabolites were identified by GC/TOF MS and high performance liquid chromatography, and these metabolites were mostly intermediates of the central carbon metabolism. The metabolite profiles of iETS3 and BY4741, cultured with or without ethanol, were significantly different based on principal component and hierarchical clustering analyses. Our metabolomic analyses identified the compositional changes in cell membranes and the activation of glutamate metabolism and the trehalose synthetic pathway as the possible mechanisms for the ethanol tolerance. These metabolic traits can be considered possible targets for further improvement of ethanol tolerance in the mutant. For example, the KGD1 deletion mutant, with up-regulated glutamate metabolism, showed increased tolerance to ethanol. This study has demonstrated that metabolomics can be a useful tool for strain improvement and phenotypic analysis of microorganisms under stress.

KW - Ethanol tolerance

KW - Metabolite profiling

KW - Metabolomics

KW - Saccharomyces cerevisiae

UR - http://www.scopus.com/inward/record.url?scp=84985972810&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84985972810&partnerID=8YFLogxK

U2 - 10.1002/biot.201500613

DO - 10.1002/biot.201500613

M3 - Article

VL - 11

SP - 1221

EP - 1229

JO - Biotechnology Journal

JF - Biotechnology Journal

SN - 1860-6768

IS - 9

ER -