Embryonic fibroblasts promote antitumor cytotoxic effects of CD8+ T cells

Yingyu Qin, Jung Hoon Shin, Jeong Ho Yoon, Se-Ho Park

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Adoptive CD8+ T cell therapy has emerged as an important modality for the treatment of cancers. However, the significant drawback of transfused T cells is their poor survival and functionality in response to tumors. To overcome this limitation, an important consideration is exploring a culture condition to generate superior antitumor cytotoxic T lymphocytes (CTLs) for adoptive therapy. Here, we provide a novel approach to generate potent CTL clones in mouse embryonic fibroblast-conditioned medium (MEF-CM). We found CTLs derived with MEF-CM have higher potential in long-term persistence in tumor bearing and non-tumor-bearing mice. Importantly, adoptive transfer of MEF-CM-cultured CTLs dramatically regressed tumor growth and prolonged mice survival. Characterization of MEF-CM-cultured CTLs (effector molecules, phenotypes, and transcription factors) suggests that MEF-CM enhances the effector functions of CD8+ T cells in part by the upregulation of the T-box transcription factor eomesodermin. Consequently, MEF-CM enhances the intrinsic qualities of effector CD8+ T cells to augment antitumor immunity.

Original languageEnglish
Article number685
JournalFrontiers in Immunology
Volume9
Issue numberAPR
DOIs
Publication statusPublished - 2018 Apr 13

Fingerprint

Conditioned Culture Medium
Fibroblasts
Cytotoxic T-Lymphocytes
T-Lymphocytes
Neoplasms
Transcription Factors
Adoptive Transfer
Cell- and Tissue-Based Therapy
Immunity
Up-Regulation
Clone Cells
Phenotype
Therapeutics
Growth

Keywords

  • Adoptive T cell therapy
  • CD8 T cells
  • Cytotoxic T lymphocytes
  • Long-term persistence
  • Mouse embryonic fibroblast-conditioned medium

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Cite this

Embryonic fibroblasts promote antitumor cytotoxic effects of CD8+ T cells. / Qin, Yingyu; Shin, Jung Hoon; Yoon, Jeong Ho; Park, Se-Ho.

In: Frontiers in Immunology, Vol. 9, No. APR, 685, 13.04.2018.

Research output: Contribution to journalArticle

Qin, Yingyu ; Shin, Jung Hoon ; Yoon, Jeong Ho ; Park, Se-Ho. / Embryonic fibroblasts promote antitumor cytotoxic effects of CD8+ T cells. In: Frontiers in Immunology. 2018 ; Vol. 9, No. APR.
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