Endogenous hydrogen sulfide production is essential for dietary restriction benefits

Christopher Hine, Eylul Harputlugil, Yue Zhang, Christoph Ruckenstuhl, Byung Cheon Lee, Lear Brace, Alban Longchamp, Jose H. Treviño-Villarreal, Pedro Mejia, C. Keith Ozaki, Rui Wang, Vadim N. Gladyshev, Frank Madeo, William B. Mair, James R. Mitchell

Research output: Contribution to journalArticle

169 Citations (Scopus)

Abstract

Dietary restriction (DR) without malnutrition encompasses numerous regimens with overlapping benefits including longevity and stress resistance, but unifying nutritional and molecular mechanisms remain elusive. In a mouse model of DR-mediated stress resistance, we found that sulfur amino acid (SAA) restriction increased expression of the transsulfuration pathway (TSP) enzyme cystathionine γ-lyase (CGL), resulting in increased hydrogen sulfide (H2S) production and protection from hepatic ischemia reperfusion injury. SAA supplementation, mTORC1 activation, or chemical/genetic CGL inhibition reduced H2S production and blocked DR-mediated stress resistance. In vitro, the mitochondrial protein SQR was required for H2S-mediated protection during nutrient/oxygen deprivation. Finally, TSP-dependent H2S production was observed in yeast, worm, fruit fly, and rodent models of DR-mediated longevity. Together, these data are consistent with evolutionary conservation of TSP-mediated H2S as a mediator of DR benefits with broad implications for clinical translation. PaperFlick

Original languageEnglish
Pages (from-to)132-144
Number of pages13
JournalCell
Volume160
Issue number1-2
DOIs
Publication statusPublished - 2015 Jan 15
Externally publishedYes

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Sulfur Amino Acids
Hydrogen Sulfide
Cystathionine
Lyases
Mitochondrial Proteins
Reperfusion Injury
Diptera
Malnutrition
Rodentia
Fruit
Yeasts
Oxygen
Fruits
Food
Yeast
Nutrients
Liver
Conservation
Enzymes
Chemical activation

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Hine, C., Harputlugil, E., Zhang, Y., Ruckenstuhl, C., Lee, B. C., Brace, L., ... Mitchell, J. R. (2015). Endogenous hydrogen sulfide production is essential for dietary restriction benefits. Cell, 160(1-2), 132-144. https://doi.org/10.1016/j.cell.2014.11.048

Endogenous hydrogen sulfide production is essential for dietary restriction benefits. / Hine, Christopher; Harputlugil, Eylul; Zhang, Yue; Ruckenstuhl, Christoph; Lee, Byung Cheon; Brace, Lear; Longchamp, Alban; Treviño-Villarreal, Jose H.; Mejia, Pedro; Ozaki, C. Keith; Wang, Rui; Gladyshev, Vadim N.; Madeo, Frank; Mair, William B.; Mitchell, James R.

In: Cell, Vol. 160, No. 1-2, 15.01.2015, p. 132-144.

Research output: Contribution to journalArticle

Hine, C, Harputlugil, E, Zhang, Y, Ruckenstuhl, C, Lee, BC, Brace, L, Longchamp, A, Treviño-Villarreal, JH, Mejia, P, Ozaki, CK, Wang, R, Gladyshev, VN, Madeo, F, Mair, WB & Mitchell, JR 2015, 'Endogenous hydrogen sulfide production is essential for dietary restriction benefits', Cell, vol. 160, no. 1-2, pp. 132-144. https://doi.org/10.1016/j.cell.2014.11.048
Hine C, Harputlugil E, Zhang Y, Ruckenstuhl C, Lee BC, Brace L et al. Endogenous hydrogen sulfide production is essential for dietary restriction benefits. Cell. 2015 Jan 15;160(1-2):132-144. https://doi.org/10.1016/j.cell.2014.11.048
Hine, Christopher ; Harputlugil, Eylul ; Zhang, Yue ; Ruckenstuhl, Christoph ; Lee, Byung Cheon ; Brace, Lear ; Longchamp, Alban ; Treviño-Villarreal, Jose H. ; Mejia, Pedro ; Ozaki, C. Keith ; Wang, Rui ; Gladyshev, Vadim N. ; Madeo, Frank ; Mair, William B. ; Mitchell, James R. / Endogenous hydrogen sulfide production is essential for dietary restriction benefits. In: Cell. 2015 ; Vol. 160, No. 1-2. pp. 132-144.
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