Endogenous neurogenesis and neovascularization in the neocortex of the rat after focal cerebral ischemia

Young Shin Hye, Hyun Kim Jin, Hoon Phi Ji, Chul K. Park, Eun Kim Jung, Jong-Hoon Kim, Ha Paek Sun, Kyu Chang Wang, Gyu Kim Dong

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

The present study was designed to examine whether endogenous neurogenesis and neovascularization occur in the neocortex of the ischemic rat brain after unilateral middle cerebral artery occlusion (MCAO). Sprague-Dawley rats were divided into six groups (n = 29): one control group (n = 4) and five groups composed of animals sacrificed at increasing times post-MCAO (2 days and 1, 2, 4, and 8 weeks; n = 5 per group). To determine the presence of neurogenesis and neovascularization in the ischemic brain, nestin, Tuj1, NeuN, GFAR Tie2, RECA, and 5-bromo-2′-deoxyuridine (BrdU) were analyzed immunohistochemically. In addition, nestin, GFAR and Tie2 expression was determined by Western blotting. Triple-labeling of nestin, BrdU, and laminin was performed to visualize the interaction between endogenous neurogenesis and neovascularization. The number of BrdU- and nestin-colabeled cells increased markedly in the neocortex and border zone of the ischemic area up to 1 week after MCAO and decreased thereafter. Western blot analysis revealed that the expression of nestin, Tie-2, and GFAP was amplified in the ipsilateral hemisphere 2 days after MCAO and peaked 1 week after MCAO, compared with that in the normal brain. After ischemic injury, nestin- and BrdU-colabeled cells were observed in the vicinity of the endothelial cells lining cerebral vessels in the ipsilateral neocortex of the ischemic brain. Endogenous neurogenesis and neovascularization were substantially activated and occurred in close proximity to one other in the ipsilateral neocortex of the ischemic rat brain.

Original languageEnglish
Pages (from-to)356-367
Number of pages12
JournalJournal of Neuroscience Research
Volume86
Issue number2
DOIs
Publication statusPublished - 2008 Feb 1

Fingerprint

Nestin
Neocortex
Neurogenesis
Brain Ischemia
Middle Cerebral Artery Infarction
Deoxyuridine
Brain
Western Blotting
Laminin
Bromodeoxyuridine
Sprague Dawley Rats
Endothelial Cells
Control Groups
Wounds and Injuries

Keywords

  • Endogenous neurogenesis
  • Ischemia
  • Neocortex
  • Neovascularization

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Endogenous neurogenesis and neovascularization in the neocortex of the rat after focal cerebral ischemia. / Hye, Young Shin; Jin, Hyun Kim; Ji, Hoon Phi; Park, Chul K.; Jung, Eun Kim; Kim, Jong-Hoon; Sun, Ha Paek; Wang, Kyu Chang; Dong, Gyu Kim.

In: Journal of Neuroscience Research, Vol. 86, No. 2, 01.02.2008, p. 356-367.

Research output: Contribution to journalArticle

Hye, Young Shin ; Jin, Hyun Kim ; Ji, Hoon Phi ; Park, Chul K. ; Jung, Eun Kim ; Kim, Jong-Hoon ; Sun, Ha Paek ; Wang, Kyu Chang ; Dong, Gyu Kim. / Endogenous neurogenesis and neovascularization in the neocortex of the rat after focal cerebral ischemia. In: Journal of Neuroscience Research. 2008 ; Vol. 86, No. 2. pp. 356-367.
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