Endogenous TRPM4-like channel in Chinese hamster ovary (CHO) cells

Oleg V. Yarishkin, Eun Mi Hwang, Jae-Yong Park, Dawon Kang, Jaehee Han, Seong Geun Hong

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Chinese hamster ovary (CHO) cells used in many transfection studies have been found to endogenously express channels permeable to monovalent cations, but not to divalent cations. In the presence of intracellular Ca 2+ , 23-pS channel with a linear current-voltage (I-V) relationship could be frequently observed in inside-out patches but not in cell-attached patches. The open probability was voltage-dependent, which is higher at positive potentials. The channel was dose-dependently activated by relatively high level of Ca 2+ (EC 50 = 1.04 ± 0.08 mM), and sensitively inhibited by 100 μM ATP, ADP, AMP, and 1 mM spermine. However, ruthenium red (2 μM) had no effect. Reverse transcript polymerase chain reaction (RT-PCR) supported the presence of mRNA encoding TRPM4b channel protein. Western blot assay finally confirmed the presence of this channel protein in membrane fraction of CHO cells. These results provide evidence that CHO cells express an endogenous TRPM4b-like channel, and thereby can be used as a tool to study de novo regulation/modulation of TRPM4 channel.

Original languageEnglish
Pages (from-to)712-717
Number of pages6
JournalBiochemical and biophysical research communications
Volume369
Issue number2
DOIs
Publication statusPublished - 2008 May 2
Externally publishedYes

Fingerprint

Cricetulus
Ovary
Cells
Ruthenium Red
Monovalent Cations
Spermine
Deregulation
Polymerase chain reaction
Divalent Cations
Electric potential
Adenosine Monophosphate
Adenosine Diphosphate
Assays
Proteins
Adenosine Triphosphate
Modulation
Membranes
Messenger RNA
Transfection
Membrane Proteins

Keywords

  • CHO cell
  • Nonselective cation channel
  • TRPM4b

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Endogenous TRPM4-like channel in Chinese hamster ovary (CHO) cells. / Yarishkin, Oleg V.; Hwang, Eun Mi; Park, Jae-Yong; Kang, Dawon; Han, Jaehee; Hong, Seong Geun.

In: Biochemical and biophysical research communications, Vol. 369, No. 2, 02.05.2008, p. 712-717.

Research output: Contribution to journalArticle

Yarishkin, Oleg V. ; Hwang, Eun Mi ; Park, Jae-Yong ; Kang, Dawon ; Han, Jaehee ; Hong, Seong Geun. / Endogenous TRPM4-like channel in Chinese hamster ovary (CHO) cells. In: Biochemical and biophysical research communications. 2008 ; Vol. 369, No. 2. pp. 712-717.
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