Endoplasmic reticulum- and Golgi-localized phospholipase A2 plays critical roles in Arabidopsis pollen development and germination

Hae Jin Kim, Sung Han Ok, Sung Chul Bahn, Juno Jang, Sung Aeong Oh, Soon Ki Park, David Twell, Stephen Beungtae Ryu, Jeong Sheop Shin

Research output: Contribution to journalArticle

42 Citations (Scopus)

Abstract

The phospholipase A2 (PLA2) superfamily of lipolytic enzymes is involved in a number of essential biological processes, such as inflammation, development, host defense, and signal transduction. Despite the proven involvement of plant PLA2s in many biological functions, including senescence, wounding, elicitor and stress responses, and pathogen defense, relatively little is known about plant PLA2s, and their genes essentially remain uncharacterized. We characterized three of four Arabidopsis thaliana PLA2 paralogs (PLA2-β, -γ, and -δ) and found that they (1) are expressed during pollen development, (2) localize to the endoplasmic reticulum and/or Golgi, and (3) play critical roles in pollen development and germination and tube growth. The suppression of PLA2 using the RNA interference approach resulted in pollen lethality. The inhibition of pollen germination by pharmacological PLA2 inhibitors was rescued by a lipid signal molecule, lysophosphatidyl ethanolamine. Based on these results, we propose that plant reproduction, in particular, male gametophyte development, requires the activities of the lipid-modifying PLA2s that are conserved in other organisms.

Original languageEnglish
Pages (from-to)94-110
Number of pages17
JournalPlant Cell
Volume23
Issue number1
DOIs
Publication statusPublished - 2011 Jan 1

Fingerprint

phospholipase A2
Phospholipases A2
Germination
Pollen
Arabidopsis
Endoplasmic Reticulum
endoplasmic reticulum
pollen
germination
Phospholipase A2 Inhibitors
Biological Phenomena
Lipids
RNA Interference
plant reproduction
ethanolamine
pollen germination
Reproduction
Signal Transduction
lipids
RNA interference

ASJC Scopus subject areas

  • Plant Science
  • Cell Biology

Cite this

Endoplasmic reticulum- and Golgi-localized phospholipase A2 plays critical roles in Arabidopsis pollen development and germination. / Kim, Hae Jin; Ok, Sung Han; Bahn, Sung Chul; Jang, Juno; Oh, Sung Aeong; Park, Soon Ki; Twell, David; Ryu, Stephen Beungtae; Shin, Jeong Sheop.

In: Plant Cell, Vol. 23, No. 1, 01.01.2011, p. 94-110.

Research output: Contribution to journalArticle

Kim, Hae Jin ; Ok, Sung Han ; Bahn, Sung Chul ; Jang, Juno ; Oh, Sung Aeong ; Park, Soon Ki ; Twell, David ; Ryu, Stephen Beungtae ; Shin, Jeong Sheop. / Endoplasmic reticulum- and Golgi-localized phospholipase A2 plays critical roles in Arabidopsis pollen development and germination. In: Plant Cell. 2011 ; Vol. 23, No. 1. pp. 94-110.
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