Endothelial nitric oxide synthase gene is associated with vessel stenosis in Korean population

Suin Yoon, Chol Shin, Hyun Young Park, Jesung Moon, Eunkyung Kim, Heung Tae Kim, Jiho Min, Sangmee Ahn Jo, Inho Jo

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Background: Nitric oxide (NO) produced by endothelial NO synthase (eNOS) mediates endothelium-dependent vasodilation and antithrombotic action. Controversial results regarding the association of eNOS gene polymorphisms with vessel stenosis have been reported. Methods: Age- and sex-matched 932 individuals (656 subjects having 1-, 2-, and 3-stenosed vessels and 276 controls without stenosis) living in Seoul and surrounding suburbs were selected. A GT missense mutation in exon 7 (894GT) was screened using PCR-restriction fragment length polymorphism analysis. The genotypes of a 27-bp insertion/deletion in intron 4 (eNOS4b/a) and a TC mutation in promoter region of -786 (-786TC) were determined by the banding pattern on gel electrophoresis and a commercially available minisequencing protocol (SNaPshot), respectively. Results: The eNOS4a allele was highly linked to the -786C allele (r=0.93, P<0.0001) while there was no linkage between eNOS4a allele and 894T allele or between 894T allele and -786C allele. Furthermore, 894T allele, but not eNOS4a (-786C) allele, was associated with the presence, but not the number, of stenosed vessels (odds ratio=1.57 for dominant effect of the T allele, P<0.05, and 1.49 for additive effect, P<0.05). Multiple logistic regression analysis revealed that 894T allele and hypertension were predictive independent risk factors for the presence of vessel stenosis. Conclusion: Our data suggest that eNOS gene polymorphisms may play an important role in the pathogenesis of vessel stenosis in Korean population.

Original languageEnglish
Pages (from-to)177-185
Number of pages9
JournalClinica Chimica Acta
Volume353
Issue number1-2
DOIs
Publication statusPublished - 2005 Mar 1

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Nitric Oxide Synthase Type III
Polymorphism
Pathologic Constriction
Genes
Alleles
Nitric Oxide Synthase
Population
Electrophoresis
Genetic Promoter Regions
Regression analysis
Introns
Logistics
Exons
Nitric Oxide
Gels
Association reactions
Missense Mutation
Vasodilation
Restriction Fragment Length Polymorphisms
Endothelium

Keywords

  • Endothelial nitric oxide synthase gene
  • Korea
  • Polymorphism
  • Vessel stenosis

ASJC Scopus subject areas

  • Biochemistry
  • Clinical Biochemistry

Cite this

Endothelial nitric oxide synthase gene is associated with vessel stenosis in Korean population. / Yoon, Suin; Shin, Chol; Park, Hyun Young; Moon, Jesung; Kim, Eunkyung; Kim, Heung Tae; Min, Jiho; Jo, Sangmee Ahn; Jo, Inho.

In: Clinica Chimica Acta, Vol. 353, No. 1-2, 01.03.2005, p. 177-185.

Research output: Contribution to journalArticle

Yoon, S, Shin, C, Park, HY, Moon, J, Kim, E, Kim, HT, Min, J, Jo, SA & Jo, I 2005, 'Endothelial nitric oxide synthase gene is associated with vessel stenosis in Korean population', Clinica Chimica Acta, vol. 353, no. 1-2, pp. 177-185. https://doi.org/10.1016/j.cccn.2004.10.018
Yoon, Suin ; Shin, Chol ; Park, Hyun Young ; Moon, Jesung ; Kim, Eunkyung ; Kim, Heung Tae ; Min, Jiho ; Jo, Sangmee Ahn ; Jo, Inho. / Endothelial nitric oxide synthase gene is associated with vessel stenosis in Korean population. In: Clinica Chimica Acta. 2005 ; Vol. 353, No. 1-2. pp. 177-185.
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T1 - Endothelial nitric oxide synthase gene is associated with vessel stenosis in Korean population

AU - Yoon, Suin

AU - Shin, Chol

AU - Park, Hyun Young

AU - Moon, Jesung

AU - Kim, Eunkyung

AU - Kim, Heung Tae

AU - Min, Jiho

AU - Jo, Sangmee Ahn

AU - Jo, Inho

PY - 2005/3/1

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N2 - Background: Nitric oxide (NO) produced by endothelial NO synthase (eNOS) mediates endothelium-dependent vasodilation and antithrombotic action. Controversial results regarding the association of eNOS gene polymorphisms with vessel stenosis have been reported. Methods: Age- and sex-matched 932 individuals (656 subjects having 1-, 2-, and 3-stenosed vessels and 276 controls without stenosis) living in Seoul and surrounding suburbs were selected. A GT missense mutation in exon 7 (894GT) was screened using PCR-restriction fragment length polymorphism analysis. The genotypes of a 27-bp insertion/deletion in intron 4 (eNOS4b/a) and a TC mutation in promoter region of -786 (-786TC) were determined by the banding pattern on gel electrophoresis and a commercially available minisequencing protocol (SNaPshot), respectively. Results: The eNOS4a allele was highly linked to the -786C allele (r=0.93, P<0.0001) while there was no linkage between eNOS4a allele and 894T allele or between 894T allele and -786C allele. Furthermore, 894T allele, but not eNOS4a (-786C) allele, was associated with the presence, but not the number, of stenosed vessels (odds ratio=1.57 for dominant effect of the T allele, P<0.05, and 1.49 for additive effect, P<0.05). Multiple logistic regression analysis revealed that 894T allele and hypertension were predictive independent risk factors for the presence of vessel stenosis. Conclusion: Our data suggest that eNOS gene polymorphisms may play an important role in the pathogenesis of vessel stenosis in Korean population.

AB - Background: Nitric oxide (NO) produced by endothelial NO synthase (eNOS) mediates endothelium-dependent vasodilation and antithrombotic action. Controversial results regarding the association of eNOS gene polymorphisms with vessel stenosis have been reported. Methods: Age- and sex-matched 932 individuals (656 subjects having 1-, 2-, and 3-stenosed vessels and 276 controls without stenosis) living in Seoul and surrounding suburbs were selected. A GT missense mutation in exon 7 (894GT) was screened using PCR-restriction fragment length polymorphism analysis. The genotypes of a 27-bp insertion/deletion in intron 4 (eNOS4b/a) and a TC mutation in promoter region of -786 (-786TC) were determined by the banding pattern on gel electrophoresis and a commercially available minisequencing protocol (SNaPshot), respectively. Results: The eNOS4a allele was highly linked to the -786C allele (r=0.93, P<0.0001) while there was no linkage between eNOS4a allele and 894T allele or between 894T allele and -786C allele. Furthermore, 894T allele, but not eNOS4a (-786C) allele, was associated with the presence, but not the number, of stenosed vessels (odds ratio=1.57 for dominant effect of the T allele, P<0.05, and 1.49 for additive effect, P<0.05). Multiple logistic regression analysis revealed that 894T allele and hypertension were predictive independent risk factors for the presence of vessel stenosis. Conclusion: Our data suggest that eNOS gene polymorphisms may play an important role in the pathogenesis of vessel stenosis in Korean population.

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