Endothelin-1 expression by vascular endothelial growth factor in human umbilical vein endothelial cells and aortic smooth muscle cells

Hyun Joo Seol, Min Jeong Oh, Hai Joong Kim

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Objective. This study investigated endothelin-1 (ET-1) production induced by vascular endothelial growth factor (VEGF) in two different vascular wall cell types. Methods. We analyzed the effect of endothelin-converting enzyme-1 (ECE-1) inhibitor and tissue inhibitors of matrix metalloproteinase-2 (TIMP-2) on VEGF-induced ET-1 expression using real-time polymerase chain reaction and enzyme-linked immunosorbent assay in human umbilical vein endothelial cells and aortic smooth muscle cells. Results. In human umbilical vein endothelial cells, both phosphoramidon, an inhibitor of ECE-1, and TIMP-2 decreased VEGF-induced ET-1 production. In aortic smooth muscle cells, TIMP-2 suppressed VEGF-induced ET-1 production, but phosphoramidon did not influence ET-1 concentration in culture. Conclusion. VEGF-induced ET-1 production may be MMP-2- or ECE-1-dependant in endothelial cells; however, in smooth muscle cells, ET-1 expression appears to be induced by MMP-2 only.

Original languageEnglish
Pages (from-to)295-301
Number of pages7
JournalHypertension in Pregnancy
Volume30
Issue number3
DOIs
Publication statusPublished - 2011 Aug 1

Fingerprint

Human Umbilical Vein Endothelial Cells
Endothelin-1
Vascular Endothelial Growth Factor A
Smooth Muscle Myocytes
Tissue Inhibitor of Metalloproteinase-2
Matrix Metalloproteinase 2
Matrix Metalloproteinases
Enzyme Inhibitors
Cell Wall
Blood Vessels
Real-Time Polymerase Chain Reaction
Endothelial Cells
Enzyme-Linked Immunosorbent Assay
Muscles
Endothelin-Converting Enzymes

Keywords

  • Aortic smooth muscle cells
  • Endothelin-1
  • Human umbilical vein endothelial cells
  • Vascular endothelial growth factor

ASJC Scopus subject areas

  • Obstetrics and Gynaecology
  • Internal Medicine

Cite this

@article{1cc7bf0ce06c401687a8402cfd610c0b,
title = "Endothelin-1 expression by vascular endothelial growth factor in human umbilical vein endothelial cells and aortic smooth muscle cells",
abstract = "Objective. This study investigated endothelin-1 (ET-1) production induced by vascular endothelial growth factor (VEGF) in two different vascular wall cell types. Methods. We analyzed the effect of endothelin-converting enzyme-1 (ECE-1) inhibitor and tissue inhibitors of matrix metalloproteinase-2 (TIMP-2) on VEGF-induced ET-1 expression using real-time polymerase chain reaction and enzyme-linked immunosorbent assay in human umbilical vein endothelial cells and aortic smooth muscle cells. Results. In human umbilical vein endothelial cells, both phosphoramidon, an inhibitor of ECE-1, and TIMP-2 decreased VEGF-induced ET-1 production. In aortic smooth muscle cells, TIMP-2 suppressed VEGF-induced ET-1 production, but phosphoramidon did not influence ET-1 concentration in culture. Conclusion. VEGF-induced ET-1 production may be MMP-2- or ECE-1-dependant in endothelial cells; however, in smooth muscle cells, ET-1 expression appears to be induced by MMP-2 only.",
keywords = "Aortic smooth muscle cells, Endothelin-1, Human umbilical vein endothelial cells, Vascular endothelial growth factor",
author = "Seol, {Hyun Joo} and Oh, {Min Jeong} and Kim, {Hai Joong}",
year = "2011",
month = "8",
day = "1",
doi = "10.3109/10641950903115053",
language = "English",
volume = "30",
pages = "295--301",
journal = "Hypertension in Pregnancy",
issn = "1064-1955",
publisher = "Informa Healthcare",
number = "3",

}

TY - JOUR

T1 - Endothelin-1 expression by vascular endothelial growth factor in human umbilical vein endothelial cells and aortic smooth muscle cells

AU - Seol, Hyun Joo

AU - Oh, Min Jeong

AU - Kim, Hai Joong

PY - 2011/8/1

Y1 - 2011/8/1

N2 - Objective. This study investigated endothelin-1 (ET-1) production induced by vascular endothelial growth factor (VEGF) in two different vascular wall cell types. Methods. We analyzed the effect of endothelin-converting enzyme-1 (ECE-1) inhibitor and tissue inhibitors of matrix metalloproteinase-2 (TIMP-2) on VEGF-induced ET-1 expression using real-time polymerase chain reaction and enzyme-linked immunosorbent assay in human umbilical vein endothelial cells and aortic smooth muscle cells. Results. In human umbilical vein endothelial cells, both phosphoramidon, an inhibitor of ECE-1, and TIMP-2 decreased VEGF-induced ET-1 production. In aortic smooth muscle cells, TIMP-2 suppressed VEGF-induced ET-1 production, but phosphoramidon did not influence ET-1 concentration in culture. Conclusion. VEGF-induced ET-1 production may be MMP-2- or ECE-1-dependant in endothelial cells; however, in smooth muscle cells, ET-1 expression appears to be induced by MMP-2 only.

AB - Objective. This study investigated endothelin-1 (ET-1) production induced by vascular endothelial growth factor (VEGF) in two different vascular wall cell types. Methods. We analyzed the effect of endothelin-converting enzyme-1 (ECE-1) inhibitor and tissue inhibitors of matrix metalloproteinase-2 (TIMP-2) on VEGF-induced ET-1 expression using real-time polymerase chain reaction and enzyme-linked immunosorbent assay in human umbilical vein endothelial cells and aortic smooth muscle cells. Results. In human umbilical vein endothelial cells, both phosphoramidon, an inhibitor of ECE-1, and TIMP-2 decreased VEGF-induced ET-1 production. In aortic smooth muscle cells, TIMP-2 suppressed VEGF-induced ET-1 production, but phosphoramidon did not influence ET-1 concentration in culture. Conclusion. VEGF-induced ET-1 production may be MMP-2- or ECE-1-dependant in endothelial cells; however, in smooth muscle cells, ET-1 expression appears to be induced by MMP-2 only.

KW - Aortic smooth muscle cells

KW - Endothelin-1

KW - Human umbilical vein endothelial cells

KW - Vascular endothelial growth factor

UR - http://www.scopus.com/inward/record.url?scp=79960152642&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=79960152642&partnerID=8YFLogxK

U2 - 10.3109/10641950903115053

DO - 10.3109/10641950903115053

M3 - Article

C2 - 21174590

AN - SCOPUS:79960152642

VL - 30

SP - 295

EP - 301

JO - Hypertension in Pregnancy

JF - Hypertension in Pregnancy

SN - 1064-1955

IS - 3

ER -