Energy conservation model based on genomic and experimental analyses of a carbon monoxide-utilizing, butyrate-forming acetogen, Eubacterium limosum KIST612

Jiyeong Jeong, Johannes Bertsch, Verena Hess, Sunju Choi, In-Geol Choi, In Seop Chang, Volker Müller

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Eubacterium limosum KIST612 is one of the few acetogens that can produce butyrate from carbon monoxide. We have used a genome-guided analysis to delineate the path of butyrate formation, the enzymes involved, and the potential coupling to ATP synthesis. Oxidation of CO is catalyzed by the acetyl-coenzyme A (CoA) synthase/CO dehydrogenase and coupled to the reduction of ferredoxin. Oxidation of reduced ferredoxin is catalyzed by the Rnf complex and Na<sup>+</sup> dependent. Consistent with the finding of a Na<sup>+</sup>-dependent Rnf complex is the presence of a conserved Na<sup>+</sup>-binding motif in the c subunit of the ATP synthase. Butyrate formation is from acetyl-CoA via acetoacetyl-CoA, hydroxybutyryl-CoA, crotonyl-CoA, and butyryl-CoA and is consistent with the finding of a gene cluster that encodes the enzymes for this pathway. The activity of the butyryl-CoA dehydrogenase was demonstrated. Reduction of crotonyl-CoA to butyryl-CoA with NADH as the reductant was coupled to reduction of ferredoxin. We postulate that the butyryl-CoA dehydrogenase uses flavin-based electron bifurcation to reduce ferredoxin, which is consistent with the finding of etfA and etfB genes next to it. The overall ATP yield was calculated and is significantly higher than the one obtained with H<inf>2</inf> + CO<inf>2</inf>. The energetic benefit may be one reason that butyrate is formed only from CO but not from H<inf>2</inf> + CO<inf>2</inf>.

Original languageEnglish
Pages (from-to)4782-4790
Number of pages9
JournalApplied and Environmental Microbiology
Volume81
Issue number14
DOIs
Publication statusPublished - 2015

Fingerprint

Eubacterium limosum
Eubacterium
Ferredoxins
energy conservation
coenzyme A
carbon monoxide
Butyrates
Carbon Monoxide
butyrates
genomics
Butyryl-CoA Dehydrogenase
Acetyl Coenzyme A
ferredoxins
Adenosine Triphosphate
carbon monoxide dehydrogenase
enzyme
oxidation
gene
bifurcation
acetyl coenzyme A

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Food Science
  • Biotechnology
  • Ecology

Cite this

Energy conservation model based on genomic and experimental analyses of a carbon monoxide-utilizing, butyrate-forming acetogen, Eubacterium limosum KIST612. / Jeong, Jiyeong; Bertsch, Johannes; Hess, Verena; Choi, Sunju; Choi, In-Geol; Chang, In Seop; Müller, Volker.

In: Applied and Environmental Microbiology, Vol. 81, No. 14, 2015, p. 4782-4790.

Research output: Contribution to journalArticle

Jeong, Jiyeong ; Bertsch, Johannes ; Hess, Verena ; Choi, Sunju ; Choi, In-Geol ; Chang, In Seop ; Müller, Volker. / Energy conservation model based on genomic and experimental analyses of a carbon monoxide-utilizing, butyrate-forming acetogen, Eubacterium limosum KIST612. In: Applied and Environmental Microbiology. 2015 ; Vol. 81, No. 14. pp. 4782-4790.
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