Engineered Enterobacter aerogenes for efficient utilization of sugarcane molasses in 2,3-butanediol production

Moo Young Jung, Bu Soo Park, Jinwon Lee, Min Kyu Oh

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

Sugarcane molasses is considered to be a good carbon source for biorefinery due to its high sugar content and low price. Sucrose occupies more than half of the sugar in the molasses. Enterobacter aerogenes is a good host strain for 2,3-butanediol production, but its utilization of sucrose is not very efficient. To improve sucrose utilization in E. aerogenes, a sucrose regulator (ScrR) was disrupted from the genomic DNA. The deletion mutation increased the sucrose consumption rate significantly when sucrose or sugarcane molasses was used as a carbon source. The 2,3-butanediol production from sugarcane molasses by the mutant was enhanced by 60% in batch fermentation compared to that by the wild type strain. In fed-batch fermentation, 98.69 g/L of 2,3-butanediol production was achieved at 36 h.

Original languageEnglish
Pages (from-to)21-27
Number of pages7
JournalBioresource technology
Volume139
DOIs
Publication statusPublished - 2013

Keywords

  • 2,3-Butanediol
  • Enterobacter aerogenes
  • Fermentation
  • Sucrose regulator
  • Sugarcane molasses

ASJC Scopus subject areas

  • Bioengineering
  • Environmental Engineering
  • Renewable Energy, Sustainability and the Environment
  • Waste Management and Disposal

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