Engineered phage matrix stiffness-modulating osteogenic differentiation

Hee Sook Lee, Jeong In Kang, Woo Jae Chung, Do Hoon Lee, Byung Yang Lee, Seung Wuk Lee, So Young Yoo

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Herein, we demonstrate an engineered phage mediated matrix for osteogenic differentiation with controlled stiffness by cross-linking the engineered phage displaying Arg-Gly-Asp (RGD) and His-Pro-Gln (HPQ) with various concentrations of streptavidin or polymer, poly(diallyldimethylammonium)chloride (PDDA). Osteogenic gene expressions showed that they were specifically increased when MC3T3 cells were cultured on the stiffer phage matrix than the softer one. Our phage matrixes can be easily functionalized using chemical/genetic engineering and used as a stem cell tissue matrix stiffness platform for modulating differential cell expansion and differentiation.

Original languageEnglish
Pages (from-to)4349-4358
Number of pages10
JournalACS Applied Materials and Interfaces
Volume10
Issue number5
DOIs
Publication statusPublished - 2018 Jan 1

Keywords

  • differentiation
  • matrix
  • osteogenic
  • phage
  • stiffness

ASJC Scopus subject areas

  • Materials Science(all)

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  • Cite this

    Lee, H. S., Kang, J. I., Chung, W. J., Lee, D. H., Lee, B. Y., Lee, S. W., & Yoo, S. Y. (2018). Engineered phage matrix stiffness-modulating osteogenic differentiation. ACS Applied Materials and Interfaces, 10(5), 4349-4358. https://doi.org/10.1021/acsami.7b17871