Engineered Zn(II)-dipicolylamine-gold nanorod provides effective prostate cancer treatment by combining siRNA delivery and photothermal therapy

Kyung Hyun Min, Young Hwa Kim, Zhantong Wang, Jihoon Kim, Jee Seon Kim, Sun Hwa Kim, Kwang Meyung Kim, Ick Chan Kwon, Dale O. Kiesewetter, Xiaoyuan Chen

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Combination cancer treatment has emerged as a critical approach to achieve remarkable anticancer effect. In this study, we prepared a theranostic nanoformulation that allows for photoacoustic imaging as well as combination gene and photothermal therapy. Gold nanorods (GNR) were coated with dipicolyl amine (DPA), which forms stable complexes with Zn2+ cations. The resulting nanoparticles, Zn(II)/DPA-GNR, recognize phosphate-containing molecules, including siRNA, because of the specific interaction between Zn(II) and the phosphates. We chose anti-polo-like kinase 1 siRNA (siPLK) as our example for gene silencing. The strong complexation between Zn(II)/DPA-GNR and siPLK provided high stability to the nano-complexes, which efficiently delivered siRNA into the targeted cancer cells in vitro and in vivo. The particle served as a theranostic agent because the GNRs of nano-complexes permitted effective photothermal therapy as well as photoacoustic imaging upon laser irradiation. This gene/photothermal combination therapy using siPLK/Zn(II)DPA-GNRs exhibited significant antitumor activity in a PC-3 tumor mouse model. The concept described in this work may be extended to the development of efficient delivery strategies for other polynucleotides as well as advanced anticancer therapy.

Original languageEnglish
Pages (from-to)4240-4254
Number of pages15
JournalTheranostics
Volume7
Issue number17
DOIs
Publication statusPublished - 2017 Jan 1
Externally publishedYes

Fingerprint

Nanotubes
Gold
Small Interfering RNA
Amines
Prostatic Neoplasms
Phosphates
Polynucleotides
Neoplasms
Gene Silencing
Therapeutics
Genetic Therapy
Nanoparticles
Cations
Lasers
zinc(II) dipicolylamine
Genes
Theranostic Nanomedicine

Keywords

  • Combination therapy
  • Dipicolylamine
  • Gold nanorod
  • Metal-organic complexes
  • Photoacoustic imaging
  • Photothermal therapy
  • siRNA
  • Theranostics

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Pharmacology, Toxicology and Pharmaceutics (miscellaneous)

Cite this

Engineered Zn(II)-dipicolylamine-gold nanorod provides effective prostate cancer treatment by combining siRNA delivery and photothermal therapy. / Min, Kyung Hyun; Kim, Young Hwa; Wang, Zhantong; Kim, Jihoon; Kim, Jee Seon; Kim, Sun Hwa; Kim, Kwang Meyung; Kwon, Ick Chan; Kiesewetter, Dale O.; Chen, Xiaoyuan.

In: Theranostics, Vol. 7, No. 17, 01.01.2017, p. 4240-4254.

Research output: Contribution to journalArticle

Min, KH, Kim, YH, Wang, Z, Kim, J, Kim, JS, Kim, SH, Kim, KM, Kwon, IC, Kiesewetter, DO & Chen, X 2017, 'Engineered Zn(II)-dipicolylamine-gold nanorod provides effective prostate cancer treatment by combining siRNA delivery and photothermal therapy', Theranostics, vol. 7, no. 17, pp. 4240-4254. https://doi.org/10.7150/thno.22435
Min, Kyung Hyun ; Kim, Young Hwa ; Wang, Zhantong ; Kim, Jihoon ; Kim, Jee Seon ; Kim, Sun Hwa ; Kim, Kwang Meyung ; Kwon, Ick Chan ; Kiesewetter, Dale O. ; Chen, Xiaoyuan. / Engineered Zn(II)-dipicolylamine-gold nanorod provides effective prostate cancer treatment by combining siRNA delivery and photothermal therapy. In: Theranostics. 2017 ; Vol. 7, No. 17. pp. 4240-4254.
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