Engineering of Klebsiella oxytoca for the Production of 2,3-Butanediol from High Concentration of Xylose

Ji Won Cha, Seung Hoon Jang, Jaewoo Son, Jungyeon Kim, Inho Jung, Kyoung Heon Kim, Yong Keun Chang, Ki Jun Jeong

Research output: Contribution to journalArticlepeer-review

Abstract

Klebsiella oxytoca is widely used for the biological production of 2,3-butanediol (2,3-BDO), a promising platform chemical with a broad range of applications. Here, to improve cell growth and production of 2,3-BDO under high concentration of xylose (100 g/L), we engineered K. oxytoca using an adaptive laboratory evolution and a biosensor-derived high throughput screening strategy. First, we developed a XylR-dependent xylose biosensor for the detection of intracellular xylose, and K. oxytoca containing the xylose biosensor was used for adaptive laboratory evolution in 100 g/L xylose. Cells were isolated by FACS screening, and the isolated strain (KO8S16) showed much improved cell growth with high xylose consumption rate (1.35 g/L/h) and 2,3-BDO productivity (0.53 g/L/h) compared with the wild-type strain. Through whole genome resequencing, it was revealed that a mutation in OmpR (a response regulator of osmotic stress) allowed to withstand high concentrations of xylose. Finally, fed-batch cultivation was performed by feeding high concentration of xylose, and K. oxytoca successfully produced 2,3-BDO at a concentration as high as 57.5 g/L by consuming 238.13 g/L xylose in 47 h.

Original languageEnglish
Pages (from-to)14395-14404
Number of pages10
JournalACS Sustainable Chemistry and Engineering
Volume9
Issue number43
DOIs
Publication statusPublished - 2021 Nov 1

Keywords

  • 2,3-butanediol
  • Klebsiella oxytoca
  • adaptive laboratory evolution
  • biosensor
  • xylose

ASJC Scopus subject areas

  • Chemistry(all)
  • Environmental Chemistry
  • Chemical Engineering(all)
  • Renewable Energy, Sustainability and the Environment

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