Engineering of plant-specific phenylpropanoids biosynthesis in Streptomyces venezuelae

Sung Ryeol Park, Jin A. Yoon, Ji Hye Paik, Je Won Park, Won Seok Jung, Yeon Hee Ban, Eun Ji Kim, Young Ji Yoo, Ah Reum Han, Yeo Joon Yoon

Research output: Contribution to journalArticlepeer-review

66 Citations (Scopus)


Phenylpropanoids, including flavonoids and stilbenes, are plant secondary metabolites with potential pharmacological and nutraceutical properties. To expand the applicability of Streptomyces venezuelae as a heterologous host to plant polyketide production, flavonoid and stilbene biosynthetic genes were expressed in an engineered strain of S. venezuelae DHS2001 bearing a deletion of native pikromycin polyketide synthase gene. A plasmid expressing the 4-coumarate/cinnamate:coenzyme A ligase from Streptomyces coelicolor (ScCCL) and the chalcone synthase from Arabidopsis thaliana (atCHS) under the control of a single ermE* promoter was constructed and introduced into S. venezuelae DHS2001. The resulting strain produced racemic naringenin and pinocembrin from 4-coumaric acid and cinnamic acid, respectively. Placement of an additional ermE* promoter upstream of the codon-optimized atCHS (atCHSop) gene significantly increased the yield of both flavanones. Expression of codon-optimized chalcone isomerase gene from Medicago sativa, together with ScCCL and atCHSop genes led to production of (2S)-flavanones, but the yield was reduced. On the other hand, a recombinant strain harboring the ScCCL and codon-optimized stilbene synthase gene from Arachis hypogaea generated stilbenes such as resveratrol and pinosylvin. This is the first report on the heterologous expression of plant phenylpropanoid biosynthetic pathways in Streptomyces genus.

Original languageEnglish
Pages (from-to)181-188
Number of pages8
JournalJournal of Biotechnology
Issue number3-4
Publication statusPublished - 2009 May 20
Externally publishedYes


  • Flavanone
  • Heterologous production
  • Stilbene
  • Streptomyces venezuelae

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology


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