Engineering of three-dimensional liver micro-tissue containing sinusoidal ultrastructure through heterotypic cell-cell interactions

D. Y. No, S. A. Lee, Sang Ho Lee

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

We developed the vascularized 3D liver-mimic micro-tissue including sinusoids and fenestrated endothelium consists of three types of liver cells by tri-culturing them on concave microwells depending on their spontaneous heterotypic interactions. The tri-cultured spheroids maintained long-term viability and functions in vitro, and showed a sinusoidal ultrastructure, allowing toxins to be secreted and supporting long life. Using this model, we also made some physiological discoveries about heterotypic interactions and fibrosis. This is the most in vivo-like liver model reported to date, and the first report about the micro-tissue containing three primary liver cells in 3D structure and having liverspecific inner structures.

Original languageEnglish
Title of host publication17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013
PublisherChemical and Biological Microsystems Society
Pages1827-1829
Number of pages3
Volume3
ISBN (Print)9781632666246
Publication statusPublished - 2013 Jan 1
Event17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013 - Freiburg, Germany
Duration: 2013 Oct 272013 Oct 31

Other

Other17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013
CountryGermany
CityFreiburg
Period13/10/2713/10/31

ASJC Scopus subject areas

  • Bioengineering

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  • Cite this

    No, D. Y., Lee, S. A., & Lee, S. H. (2013). Engineering of three-dimensional liver micro-tissue containing sinusoidal ultrastructure through heterotypic cell-cell interactions. In 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013 (Vol. 3, pp. 1827-1829). Chemical and Biological Microsystems Society.