Abstract
2,3-Butanediol (2,3-BD) is a major metabolite produced by Klebsiella pneumoniae KCTC2242, which is a important chemical with wide applications. Three genes important for 2,3-BD biosynthesis acetolactate decarboxylase (budA), acetolactate synthase (budB), and alcohol dehydrogenase (budC) were identified in K. pneumoniae genomic DNA. With the goal of enhancing 2,3-BD production, these genes were cloned into pUC18K expression vectors containing the lacZ promoter and the kanamycin resistance gene to generate plasmids pSB1-7. The plasmids were then introduced into K. pneumoniae using electroporation. All strains were incubated in flask experiments and 2,3-BD production was increased by 60% in recombinant bacteria harboring pSB04 (budA and budB genes), compared with the parental strain K. pneumoniae KCTC2242. The maximum 2,3-BD production level achieved through fedbatch fermentation with K. pneumoniae SGJSB04 was 101.53 g/l over 40 h with a productivity of 2.54 g/l.h. These results suggest that overexpression of 2,3-BD synthesisrelated genes can enhance 2,3-BD production in K. pneumoniae by fermentation.
| Original language | English |
|---|---|
| Pages (from-to) | 1258-1263 |
| Number of pages | 6 |
| Journal | Journal of Microbiology and Biotechnology |
| Volume | 22 |
| Issue number | 9 |
| DOIs | |
| Publication status | Published - 2012 Sep 28 |
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Keywords
- 2,3-Butanediol
- Acetolactate decarboxylase
- Acetolactate synthase
- Alcohol dehydrogenase
- Gene manipulation
- Klebsiella pneumoniae
ASJC Scopus subject areas
- Biotechnology
- Applied Microbiology and Biotechnology
Cite this
Enhanced 2,3-butanediol production in recombinant Klebsiella pneumoniae via overexpression of synthesis-related genes. / Kim, Borim; Lee, Soojin; Park, Joohong; Lu, Mingshou; Oh, Min-Kyu; Kim, Youngrok; Lee, Jinwon.
In: Journal of Microbiology and Biotechnology, Vol. 22, No. 9, 28.09.2012, p. 1258-1263.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Enhanced 2,3-butanediol production in recombinant Klebsiella pneumoniae via overexpression of synthesis-related genes
AU - Kim, Borim
AU - Lee, Soojin
AU - Park, Joohong
AU - Lu, Mingshou
AU - Oh, Min-Kyu
AU - Kim, Youngrok
AU - Lee, Jinwon
PY - 2012/9/28
Y1 - 2012/9/28
N2 - 2,3-Butanediol (2,3-BD) is a major metabolite produced by Klebsiella pneumoniae KCTC2242, which is a important chemical with wide applications. Three genes important for 2,3-BD biosynthesis acetolactate decarboxylase (budA), acetolactate synthase (budB), and alcohol dehydrogenase (budC) were identified in K. pneumoniae genomic DNA. With the goal of enhancing 2,3-BD production, these genes were cloned into pUC18K expression vectors containing the lacZ promoter and the kanamycin resistance gene to generate plasmids pSB1-7. The plasmids were then introduced into K. pneumoniae using electroporation. All strains were incubated in flask experiments and 2,3-BD production was increased by 60% in recombinant bacteria harboring pSB04 (budA and budB genes), compared with the parental strain K. pneumoniae KCTC2242. The maximum 2,3-BD production level achieved through fedbatch fermentation with K. pneumoniae SGJSB04 was 101.53 g/l over 40 h with a productivity of 2.54 g/l.h. These results suggest that overexpression of 2,3-BD synthesisrelated genes can enhance 2,3-BD production in K. pneumoniae by fermentation.
AB - 2,3-Butanediol (2,3-BD) is a major metabolite produced by Klebsiella pneumoniae KCTC2242, which is a important chemical with wide applications. Three genes important for 2,3-BD biosynthesis acetolactate decarboxylase (budA), acetolactate synthase (budB), and alcohol dehydrogenase (budC) were identified in K. pneumoniae genomic DNA. With the goal of enhancing 2,3-BD production, these genes were cloned into pUC18K expression vectors containing the lacZ promoter and the kanamycin resistance gene to generate plasmids pSB1-7. The plasmids were then introduced into K. pneumoniae using electroporation. All strains were incubated in flask experiments and 2,3-BD production was increased by 60% in recombinant bacteria harboring pSB04 (budA and budB genes), compared with the parental strain K. pneumoniae KCTC2242. The maximum 2,3-BD production level achieved through fedbatch fermentation with K. pneumoniae SGJSB04 was 101.53 g/l over 40 h with a productivity of 2.54 g/l.h. These results suggest that overexpression of 2,3-BD synthesisrelated genes can enhance 2,3-BD production in K. pneumoniae by fermentation.
KW - 2,3-Butanediol
KW - Acetolactate decarboxylase
KW - Acetolactate synthase
KW - Alcohol dehydrogenase
KW - Gene manipulation
KW - Klebsiella pneumoniae
UR - http://www.scopus.com/inward/record.url?scp=84864096711&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84864096711&partnerID=8YFLogxK
U2 - 10.4014/jmb.1201.01044
DO - 10.4014/jmb.1201.01044
M3 - Article
VL - 22
SP - 1258
EP - 1263
JO - Journal of Microbiology and Biotechnology
JF - Journal of Microbiology and Biotechnology
SN - 1017-7825
IS - 9
ER -