Enhanced 2,3-butanediol production in recombinant Klebsiella pneumoniae via overexpression of synthesis-related genes

Borim Kim, Soojin Lee, Joohong Park, Mingshou Lu, Min-Kyu Oh, Youngrok Kim, Jinwon Lee

Research output: Contribution to journalArticle

39 Citations (Scopus)

Abstract

2,3-Butanediol (2,3-BD) is a major metabolite produced by Klebsiella pneumoniae KCTC2242, which is a important chemical with wide applications. Three genes important for 2,3-BD biosynthesis acetolactate decarboxylase (budA), acetolactate synthase (budB), and alcohol dehydrogenase (budC) were identified in K. pneumoniae genomic DNA. With the goal of enhancing 2,3-BD production, these genes were cloned into pUC18K expression vectors containing the lacZ promoter and the kanamycin resistance gene to generate plasmids pSB1-7. The plasmids were then introduced into K. pneumoniae using electroporation. All strains were incubated in flask experiments and 2,3-BD production was increased by 60% in recombinant bacteria harboring pSB04 (budA and budB genes), compared with the parental strain K. pneumoniae KCTC2242. The maximum 2,3-BD production level achieved through fedbatch fermentation with K. pneumoniae SGJSB04 was 101.53 g/l over 40 h with a productivity of 2.54 g/l.h. These results suggest that overexpression of 2,3-BD synthesisrelated genes can enhance 2,3-BD production in K. pneumoniae by fermentation.

Original languageEnglish
Pages (from-to)1258-1263
Number of pages6
JournalJournal of Microbiology and Biotechnology
Volume22
Issue number9
DOIs
Publication statusPublished - 2012 Sep 28

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Keywords

  • 2,3-Butanediol
  • Acetolactate decarboxylase
  • Acetolactate synthase
  • Alcohol dehydrogenase
  • Gene manipulation
  • Klebsiella pneumoniae

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology

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