Enhanced 2,3-butanediol production in recombinant Klebsiella pneumoniae via overexpression of synthesis-related genes

Borim Kim, Soojin Lee, Joohong Park, Mingshou Lu, Min-Kyu Oh, Youngrok Kim, Jinwon Lee

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

2,3-Butanediol (2,3-BD) is a major metabolite produced by Klebsiella pneumoniae KCTC2242, which is a important chemical with wide applications. Three genes important for 2,3-BD biosynthesis acetolactate decarboxylase (budA), acetolactate synthase (budB), and alcohol dehydrogenase (budC) were identified in K. pneumoniae genomic DNA. With the goal of enhancing 2,3-BD production, these genes were cloned into pUC18K expression vectors containing the lacZ promoter and the kanamycin resistance gene to generate plasmids pSB1-7. The plasmids were then introduced into K. pneumoniae using electroporation. All strains were incubated in flask experiments and 2,3-BD production was increased by 60% in recombinant bacteria harboring pSB04 (budA and budB genes), compared with the parental strain K. pneumoniae KCTC2242. The maximum 2,3-BD production level achieved through fedbatch fermentation with K. pneumoniae SGJSB04 was 101.53 g/l over 40 h with a productivity of 2.54 g/l.h. These results suggest that overexpression of 2,3-BD synthesisrelated genes can enhance 2,3-BD production in K. pneumoniae by fermentation.

Original languageEnglish
Pages (from-to)1258-1263
Number of pages6
JournalJournal of Microbiology and Biotechnology
Volume22
Issue number9
DOIs
Publication statusPublished - 2012 Sep 28

Fingerprint

Klebsiella pneumoniae
Genes
acetolactate decarboxylase
Fermentation
Plasmids
Acetolactate Synthase
Kanamycin Resistance
Electroporation
Alcohol Dehydrogenase
2,3-butylene glycol
Bacteria
DNA

Keywords

  • 2,3-Butanediol
  • Acetolactate decarboxylase
  • Acetolactate synthase
  • Alcohol dehydrogenase
  • Gene manipulation
  • Klebsiella pneumoniae

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology

Cite this

Enhanced 2,3-butanediol production in recombinant Klebsiella pneumoniae via overexpression of synthesis-related genes. / Kim, Borim; Lee, Soojin; Park, Joohong; Lu, Mingshou; Oh, Min-Kyu; Kim, Youngrok; Lee, Jinwon.

In: Journal of Microbiology and Biotechnology, Vol. 22, No. 9, 28.09.2012, p. 1258-1263.

Research output: Contribution to journalArticle

Kim, Borim ; Lee, Soojin ; Park, Joohong ; Lu, Mingshou ; Oh, Min-Kyu ; Kim, Youngrok ; Lee, Jinwon. / Enhanced 2,3-butanediol production in recombinant Klebsiella pneumoniae via overexpression of synthesis-related genes. In: Journal of Microbiology and Biotechnology. 2012 ; Vol. 22, No. 9. pp. 1258-1263.
@article{ec151b3c81124dbaac5262806876fd25,
title = "Enhanced 2,3-butanediol production in recombinant Klebsiella pneumoniae via overexpression of synthesis-related genes",
abstract = "2,3-Butanediol (2,3-BD) is a major metabolite produced by Klebsiella pneumoniae KCTC2242, which is a important chemical with wide applications. Three genes important for 2,3-BD biosynthesis acetolactate decarboxylase (budA), acetolactate synthase (budB), and alcohol dehydrogenase (budC) were identified in K. pneumoniae genomic DNA. With the goal of enhancing 2,3-BD production, these genes were cloned into pUC18K expression vectors containing the lacZ promoter and the kanamycin resistance gene to generate plasmids pSB1-7. The plasmids were then introduced into K. pneumoniae using electroporation. All strains were incubated in flask experiments and 2,3-BD production was increased by 60{\%} in recombinant bacteria harboring pSB04 (budA and budB genes), compared with the parental strain K. pneumoniae KCTC2242. The maximum 2,3-BD production level achieved through fedbatch fermentation with K. pneumoniae SGJSB04 was 101.53 g/l over 40 h with a productivity of 2.54 g/l.h. These results suggest that overexpression of 2,3-BD synthesisrelated genes can enhance 2,3-BD production in K. pneumoniae by fermentation.",
keywords = "2,3-Butanediol, Acetolactate decarboxylase, Acetolactate synthase, Alcohol dehydrogenase, Gene manipulation, Klebsiella pneumoniae",
author = "Borim Kim and Soojin Lee and Joohong Park and Mingshou Lu and Min-Kyu Oh and Youngrok Kim and Jinwon Lee",
year = "2012",
month = "9",
day = "28",
doi = "10.4014/jmb.1201.01044",
language = "English",
volume = "22",
pages = "1258--1263",
journal = "Journal of Microbiology and Biotechnology",
issn = "1017-7825",
publisher = "Korean Society for Microbiolog and Biotechnology",
number = "9",

}

TY - JOUR

T1 - Enhanced 2,3-butanediol production in recombinant Klebsiella pneumoniae via overexpression of synthesis-related genes

AU - Kim, Borim

AU - Lee, Soojin

AU - Park, Joohong

AU - Lu, Mingshou

AU - Oh, Min-Kyu

AU - Kim, Youngrok

AU - Lee, Jinwon

PY - 2012/9/28

Y1 - 2012/9/28

N2 - 2,3-Butanediol (2,3-BD) is a major metabolite produced by Klebsiella pneumoniae KCTC2242, which is a important chemical with wide applications. Three genes important for 2,3-BD biosynthesis acetolactate decarboxylase (budA), acetolactate synthase (budB), and alcohol dehydrogenase (budC) were identified in K. pneumoniae genomic DNA. With the goal of enhancing 2,3-BD production, these genes were cloned into pUC18K expression vectors containing the lacZ promoter and the kanamycin resistance gene to generate plasmids pSB1-7. The plasmids were then introduced into K. pneumoniae using electroporation. All strains were incubated in flask experiments and 2,3-BD production was increased by 60% in recombinant bacteria harboring pSB04 (budA and budB genes), compared with the parental strain K. pneumoniae KCTC2242. The maximum 2,3-BD production level achieved through fedbatch fermentation with K. pneumoniae SGJSB04 was 101.53 g/l over 40 h with a productivity of 2.54 g/l.h. These results suggest that overexpression of 2,3-BD synthesisrelated genes can enhance 2,3-BD production in K. pneumoniae by fermentation.

AB - 2,3-Butanediol (2,3-BD) is a major metabolite produced by Klebsiella pneumoniae KCTC2242, which is a important chemical with wide applications. Three genes important for 2,3-BD biosynthesis acetolactate decarboxylase (budA), acetolactate synthase (budB), and alcohol dehydrogenase (budC) were identified in K. pneumoniae genomic DNA. With the goal of enhancing 2,3-BD production, these genes were cloned into pUC18K expression vectors containing the lacZ promoter and the kanamycin resistance gene to generate plasmids pSB1-7. The plasmids were then introduced into K. pneumoniae using electroporation. All strains were incubated in flask experiments and 2,3-BD production was increased by 60% in recombinant bacteria harboring pSB04 (budA and budB genes), compared with the parental strain K. pneumoniae KCTC2242. The maximum 2,3-BD production level achieved through fedbatch fermentation with K. pneumoniae SGJSB04 was 101.53 g/l over 40 h with a productivity of 2.54 g/l.h. These results suggest that overexpression of 2,3-BD synthesisrelated genes can enhance 2,3-BD production in K. pneumoniae by fermentation.

KW - 2,3-Butanediol

KW - Acetolactate decarboxylase

KW - Acetolactate synthase

KW - Alcohol dehydrogenase

KW - Gene manipulation

KW - Klebsiella pneumoniae

UR - http://www.scopus.com/inward/record.url?scp=84864096711&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84864096711&partnerID=8YFLogxK

U2 - 10.4014/jmb.1201.01044

DO - 10.4014/jmb.1201.01044

M3 - Article

VL - 22

SP - 1258

EP - 1263

JO - Journal of Microbiology and Biotechnology

JF - Journal of Microbiology and Biotechnology

SN - 1017-7825

IS - 9

ER -