TY - JOUR
T1 - Enhanced accumulation of gamma-aminobutyric acid in rice bran using anaerobic incubation with various additives
AU - Oh, Su Jin
AU - Kim, Hyun Soo
AU - Lim, Seung Taik
AU - Reddy, Chagam Koteswara
N1 - Funding Information:
This research was financially supported by Small and Medium Business Administration in Korea , South Korea (Grants No. R1626002 ). In addition, authors thank the BK21 PLUS program at Korea University for providing the support to carry out the research.
Funding Information:
This research was financially supported by Small and Medium Business Administration in Korea, South Korea (Grants No. R1626002). In addition, authors thank the BK21 PLUS program at Korea University for providing the support to carry out the research.
Publisher Copyright:
© 2018 Elsevier Ltd
PY - 2019/1/15
Y1 - 2019/1/15
N2 - An anaerobic incubation for the enzymatic production of gamma-aminobutyric acid (GABA) in rice bran with the addition of glutamate, hydrolyzed wheat protein (HWP), yeast extract (YE) and pyridoxal-5-phosphate (PLP) was investigated. Rice bran was moistened (30% moisture content) with an electrolyzed oxidizing water and anaerobically incubated under nitrogen at 40 °C for 8 h. The incubation activated the glutamate decarboxylase (GAD) in rice bran and increased the GABA content from 10.7 to 171.5 mg/100 g. The addition of glutamate and protein hydrolysates further amplified the GABA content in the treated rice bran: 974.9, 487.4, and 372.8 mg/100 g, with 2.25% glutamate, 6% HWP, and 8% YE, respectively. Furthermore, addition of PLP (1.48 mg/100 g) as a coenzyme for GAD, together with 2.25% glutamate addition, could raise the GABA accumulation in rice bran to 2242 mg/100 g. Other amino acids in rice bran were changed in their composition by the anaerobic treatment.
AB - An anaerobic incubation for the enzymatic production of gamma-aminobutyric acid (GABA) in rice bran with the addition of glutamate, hydrolyzed wheat protein (HWP), yeast extract (YE) and pyridoxal-5-phosphate (PLP) was investigated. Rice bran was moistened (30% moisture content) with an electrolyzed oxidizing water and anaerobically incubated under nitrogen at 40 °C for 8 h. The incubation activated the glutamate decarboxylase (GAD) in rice bran and increased the GABA content from 10.7 to 171.5 mg/100 g. The addition of glutamate and protein hydrolysates further amplified the GABA content in the treated rice bran: 974.9, 487.4, and 372.8 mg/100 g, with 2.25% glutamate, 6% HWP, and 8% YE, respectively. Furthermore, addition of PLP (1.48 mg/100 g) as a coenzyme for GAD, together with 2.25% glutamate addition, could raise the GABA accumulation in rice bran to 2242 mg/100 g. Other amino acids in rice bran were changed in their composition by the anaerobic treatment.
KW - Anaerobic incubation
KW - Gamma-aminobutyric acid
KW - Hydrolyzed wheat protein
KW - Pyridoxal-5-phosphate
KW - Rice bran
KW - Yeast extract
UR - http://www.scopus.com/inward/record.url?scp=85050670050&partnerID=8YFLogxK
U2 - 10.1016/j.foodchem.2018.07.175
DO - 10.1016/j.foodchem.2018.07.175
M3 - Article
C2 - 30236666
AN - SCOPUS:85050670050
VL - 271
SP - 187
EP - 192
JO - Food Chemistry
JF - Food Chemistry
SN - 0308-8146
ER -