Enhanced accumulation of gamma-aminobutyric acid in rice bran using anaerobic incubation with various additives

Su Jin Oh, Hyun Soo Kim, Seung Taik Lim, Chagam Koteswara Reddy

Research output: Contribution to journalArticle

Abstract

An anaerobic incubation for the enzymatic production of gamma-aminobutyric acid (GABA) in rice bran with the addition of glutamate, hydrolyzed wheat protein (HWP), yeast extract (YE) and pyridoxal-5-phosphate (PLP) was investigated. Rice bran was moistened (30% moisture content) with an electrolyzed oxidizing water and anaerobically incubated under nitrogen at 40 °C for 8 h. The incubation activated the glutamate decarboxylase (GAD) in rice bran and increased the GABA content from 10.7 to 171.5 mg/100 g. The addition of glutamate and protein hydrolysates further amplified the GABA content in the treated rice bran: 974.9, 487.4, and 372.8 mg/100 g, with 2.25% glutamate, 6% HWP, and 8% YE, respectively. Furthermore, addition of PLP (1.48 mg/100 g) as a coenzyme for GAD, together with 2.25% glutamate addition, could raise the GABA accumulation in rice bran to 2242 mg/100 g. Other amino acids in rice bran were changed in their composition by the anaerobic treatment.

LanguageEnglish
Pages187-192
Number of pages6
JournalFood Chemistry
Volume271
DOIs
Publication statusPublished - 2019 Jan 15

Fingerprint

rice bran
gamma-aminobutyric acid
gamma-Aminobutyric Acid
Glutamic Acid
glutamates
Pyridoxal Phosphate
Glutamate Decarboxylase
protein hydrolysates
glutamate decarboxylase
pyridoxal phosphate
wheat protein
Fungal Proteins
yeast extract
Protein Hydrolysates
Triticum
Coenzymes
Yeast
Nitrogen
Moisture
coenzymes

Keywords

  • Anaerobic incubation
  • Gamma-aminobutyric acid
  • Hydrolyzed wheat protein
  • Pyridoxal-5-phosphate
  • Rice bran
  • Yeast extract

ASJC Scopus subject areas

  • Analytical Chemistry
  • Food Science

Cite this

Enhanced accumulation of gamma-aminobutyric acid in rice bran using anaerobic incubation with various additives. / Oh, Su Jin; Kim, Hyun Soo; Lim, Seung Taik; Reddy, Chagam Koteswara.

In: Food Chemistry, Vol. 271, 15.01.2019, p. 187-192.

Research output: Contribution to journalArticle

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abstract = "An anaerobic incubation for the enzymatic production of gamma-aminobutyric acid (GABA) in rice bran with the addition of glutamate, hydrolyzed wheat protein (HWP), yeast extract (YE) and pyridoxal-5-phosphate (PLP) was investigated. Rice bran was moistened (30{\%} moisture content) with an electrolyzed oxidizing water and anaerobically incubated under nitrogen at 40 °C for 8 h. The incubation activated the glutamate decarboxylase (GAD) in rice bran and increased the GABA content from 10.7 to 171.5 mg/100 g. The addition of glutamate and protein hydrolysates further amplified the GABA content in the treated rice bran: 974.9, 487.4, and 372.8 mg/100 g, with 2.25{\%} glutamate, 6{\%} HWP, and 8{\%} YE, respectively. Furthermore, addition of PLP (1.48 mg/100 g) as a coenzyme for GAD, together with 2.25{\%} glutamate addition, could raise the GABA accumulation in rice bran to 2242 mg/100 g. Other amino acids in rice bran were changed in their composition by the anaerobic treatment.",
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