TY - JOUR
T1 - Enhanced antioxidant activity of rice bran extract by carbohydrase treatment
AU - Kim, Sung Min
AU - Lim, Seung Taik
N1 - Funding Information:
This study was financially supported by the Korean Small and Medium Business Administration (Grants No. S2108305 ) in 2014.
Publisher Copyright:
© 2016 Elsevier Ltd.
Copyright:
Copyright 2017 Elsevier B.V., All rights reserved.
PY - 2016/3/1
Y1 - 2016/3/1
N2 - Rice bran (RB) was treated with different carbohydrases (Viscozyme, Termamyl, Celluclast, AMG, Ultraflo, and Pentopan), and then aqueous alcoholic extracts (50% ethanol) from the treated RB were examined for their phenolic compositions and antioxidant activities (radical scavenging activity and ferrous reducing activity). All the carbohydrases tested induced significant increases in ferric reducing power (1.5-3.3 times). Among the enzymes tested, Pentopan which was active in pentosan hydrolysis appeared to be most effective in increasing the antioxidant activity. Celluclast, Ultraflo, and Pentopan appeared more effective in increasing the total phenolic content (TPC) as well as radical scavenging activity than other enzymes. These enzymes increased the amount of extractable phenolic acids by 2.5-3.0 times, especially for ferulic, p-coumaric, vanillic, and p-hydroxybenzoic acids. Enzymatic hydrolysis of cell wall polysaccharides in RB could be used as an effective procedure for raising the amount of extractable phenolic acids and thus increasing the antioxidant activity of RB extract.
AB - Rice bran (RB) was treated with different carbohydrases (Viscozyme, Termamyl, Celluclast, AMG, Ultraflo, and Pentopan), and then aqueous alcoholic extracts (50% ethanol) from the treated RB were examined for their phenolic compositions and antioxidant activities (radical scavenging activity and ferrous reducing activity). All the carbohydrases tested induced significant increases in ferric reducing power (1.5-3.3 times). Among the enzymes tested, Pentopan which was active in pentosan hydrolysis appeared to be most effective in increasing the antioxidant activity. Celluclast, Ultraflo, and Pentopan appeared more effective in increasing the total phenolic content (TPC) as well as radical scavenging activity than other enzymes. These enzymes increased the amount of extractable phenolic acids by 2.5-3.0 times, especially for ferulic, p-coumaric, vanillic, and p-hydroxybenzoic acids. Enzymatic hydrolysis of cell wall polysaccharides in RB could be used as an effective procedure for raising the amount of extractable phenolic acids and thus increasing the antioxidant activity of RB extract.
KW - Antioxidant activity
KW - Carbohydrase
KW - Phenolic acid
KW - Phenolic compound
KW - Rice bran
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U2 - 10.1016/j.jcs.2016.01.006
DO - 10.1016/j.jcs.2016.01.006
M3 - Article
AN - SCOPUS:84960851478
VL - 68
SP - 116
EP - 121
JO - Journal of Cereal Science
JF - Journal of Cereal Science
SN - 0733-5210
ER -