Abstract
Rice bran (RB) was treated with different carbohydrases (Viscozyme, Termamyl, Celluclast, AMG, Ultraflo, and Pentopan), and then aqueous alcoholic extracts (50% ethanol) from the treated RB were examined for their phenolic compositions and antioxidant activities (radical scavenging activity and ferrous reducing activity). All the carbohydrases tested induced significant increases in ferric reducing power (1.5-3.3 times). Among the enzymes tested, Pentopan which was active in pentosan hydrolysis appeared to be most effective in increasing the antioxidant activity. Celluclast, Ultraflo, and Pentopan appeared more effective in increasing the total phenolic content (TPC) as well as radical scavenging activity than other enzymes. These enzymes increased the amount of extractable phenolic acids by 2.5-3.0 times, especially for ferulic, p-coumaric, vanillic, and p-hydroxybenzoic acids. Enzymatic hydrolysis of cell wall polysaccharides in RB could be used as an effective procedure for raising the amount of extractable phenolic acids and thus increasing the antioxidant activity of RB extract.
| Original language | English |
|---|---|
| Pages (from-to) | 116-121 |
| Number of pages | 6 |
| Journal | Journal of Cereal Science |
| Volume | 68 |
| DOIs | |
| Publication status | Published - 2016 Mar 1 |
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Keywords
- Antioxidant activity
- Carbohydrase
- Phenolic acid
- Phenolic compound
- Rice bran
ASJC Scopus subject areas
- Food Science
- Biochemistry
Cite this
Enhanced antioxidant activity of rice bran extract by carbohydrase treatment. / Kim, Sung Min; Lim, Seung Taik.
In: Journal of Cereal Science, Vol. 68, 01.03.2016, p. 116-121.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Enhanced antioxidant activity of rice bran extract by carbohydrase treatment
AU - Kim, Sung Min
AU - Lim, Seung Taik
PY - 2016/3/1
Y1 - 2016/3/1
N2 - Rice bran (RB) was treated with different carbohydrases (Viscozyme, Termamyl, Celluclast, AMG, Ultraflo, and Pentopan), and then aqueous alcoholic extracts (50% ethanol) from the treated RB were examined for their phenolic compositions and antioxidant activities (radical scavenging activity and ferrous reducing activity). All the carbohydrases tested induced significant increases in ferric reducing power (1.5-3.3 times). Among the enzymes tested, Pentopan which was active in pentosan hydrolysis appeared to be most effective in increasing the antioxidant activity. Celluclast, Ultraflo, and Pentopan appeared more effective in increasing the total phenolic content (TPC) as well as radical scavenging activity than other enzymes. These enzymes increased the amount of extractable phenolic acids by 2.5-3.0 times, especially for ferulic, p-coumaric, vanillic, and p-hydroxybenzoic acids. Enzymatic hydrolysis of cell wall polysaccharides in RB could be used as an effective procedure for raising the amount of extractable phenolic acids and thus increasing the antioxidant activity of RB extract.
AB - Rice bran (RB) was treated with different carbohydrases (Viscozyme, Termamyl, Celluclast, AMG, Ultraflo, and Pentopan), and then aqueous alcoholic extracts (50% ethanol) from the treated RB were examined for their phenolic compositions and antioxidant activities (radical scavenging activity and ferrous reducing activity). All the carbohydrases tested induced significant increases in ferric reducing power (1.5-3.3 times). Among the enzymes tested, Pentopan which was active in pentosan hydrolysis appeared to be most effective in increasing the antioxidant activity. Celluclast, Ultraflo, and Pentopan appeared more effective in increasing the total phenolic content (TPC) as well as radical scavenging activity than other enzymes. These enzymes increased the amount of extractable phenolic acids by 2.5-3.0 times, especially for ferulic, p-coumaric, vanillic, and p-hydroxybenzoic acids. Enzymatic hydrolysis of cell wall polysaccharides in RB could be used as an effective procedure for raising the amount of extractable phenolic acids and thus increasing the antioxidant activity of RB extract.
KW - Antioxidant activity
KW - Carbohydrase
KW - Phenolic acid
KW - Phenolic compound
KW - Rice bran
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U2 - 10.1016/j.jcs.2016.01.006
DO - 10.1016/j.jcs.2016.01.006
M3 - Article
VL - 68
SP - 116
EP - 121
JO - Journal of Cereal Science
JF - Journal of Cereal Science
SN - 0733-5210
ER -