Enhanced antitumor effects by combination gene therapy using MDR1 gene shRNA and HSV1-tk in a xenograft mouse model

Sang Woo Lee, You La Lee, Yong Jin Lee, Seung Yoon Park, In-San Kim, Tae Hyun Choi, Jeoung Hee Ha, Byeong Cheol Ahn, Jaetae Lee

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

The use of a novel therapeutic vector containing HSV1-thymidine kinase (HSV1-tk) and a short hairpin RNA for the MDR1 gene (shMDR) was proposed previously. We investigated the antitumor effects in an in vivo mouse model of colon cancer and assessed treatment response by serial non-invasive imaging. shMDR-TK expressing (MTKG) tumors for the dual therapy group mice with ganciclovir and doxorubicin showed a decrease in size, while tumors in the single therapy group mice showed a moderate increase (p < 0.05). The 131 I-5-iodo-2′-fluoro-2′deoxy-1-β-d-arabinofuranosyluracil (FIAU) uptake ratio of MTKG-to-parent HCT-15 tumors decreased as treatment progressed for single or dual therapy group mice, while that of the control group mice increased gradually. This study demonstrates the enhanced antitumor effects with combination gene therapy compared with a single therapeutic approach, and provides the potential of therapeutic response monitoring.

Original languageEnglish
Pages (from-to)83-89
Number of pages7
JournalCancer letters
Volume291
Issue number1
DOIs
Publication statusPublished - 2010 May 1
Externally publishedYes

Fingerprint

Thymidine Kinase
Heterografts
Genetic Therapy
Small Interfering RNA
Group Psychotherapy
Genes
Arabinofuranosyluracil
Therapeutics
Neoplasms
Ganciclovir
Doxorubicin
Colonic Neoplasms
Control Groups

Keywords

  • I-FIAU imaging
  • Combination gene therapy
  • HSV1-tk
  • MDR1
  • shRNA

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Cite this

Enhanced antitumor effects by combination gene therapy using MDR1 gene shRNA and HSV1-tk in a xenograft mouse model. / Lee, Sang Woo; Lee, You La; Lee, Yong Jin; Park, Seung Yoon; Kim, In-San; Choi, Tae Hyun; Ha, Jeoung Hee; Ahn, Byeong Cheol; Lee, Jaetae.

In: Cancer letters, Vol. 291, No. 1, 01.05.2010, p. 83-89.

Research output: Contribution to journalArticle

Lee, Sang Woo ; Lee, You La ; Lee, Yong Jin ; Park, Seung Yoon ; Kim, In-San ; Choi, Tae Hyun ; Ha, Jeoung Hee ; Ahn, Byeong Cheol ; Lee, Jaetae. / Enhanced antitumor effects by combination gene therapy using MDR1 gene shRNA and HSV1-tk in a xenograft mouse model. In: Cancer letters. 2010 ; Vol. 291, No. 1. pp. 83-89.
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