Abstract
Haematococcus pluvialis is the richest source of natural astaxanthin (3S, 3′S), but the rigid cell wall of mature red cyst (aplanospore) complicates the efficient extraction of astaxanthin from the strain. Herein, the cyst germination method was developed by using nitrate and light for practical application for an efficient astaxanthin extraction from Haematococcus cells cultured in outdoor condition where flue gas, solar radiation and photobioreactor were used. Notably, autotrophic germination rate was easily regulated by light intensity. Under conditions of low germination rate, total astaxanthin concentration was highly maintained and astaxanthin extraction efficiency was rapidly enhanced during autotrophic germination. As a result, under homogenization (30 s), the extracted astaxanthin concentration in the cells treated with 1 mM KNO3-150 μE/m2/s was highly increased by 58% compared to the cells treated without germination. Our technical solution will definitely improve an astaxanthin extraction titer with the practical application in outdoor Haematococcus culture system.
| Original language | English |
|---|---|
| Pages (from-to) | 2275-2280 |
| Number of pages | 6 |
| Journal | Process Biochemistry |
| Volume | 50 |
| Issue number | 12 |
| DOIs | |
| Publication status | Published - 2015 Dec 1 |
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Keywords
- Astaxanthin
- Cyst germination
- Haematococcus pluvialis
- Nitrate and solar radiation
- Outdoor autotrophic culture
ASJC Scopus subject areas
- Biochemistry
- Applied Microbiology and Biotechnology
- Bioengineering
Cite this
Enhanced astaxanthin extraction efficiency from Haematococcus pluvialis via the cyst germination in outdoor culture systems. / Choi, Yoon Young; Hong, Min Eui; Sim, Sang Jun.
In: Process Biochemistry, Vol. 50, No. 12, 01.12.2015, p. 2275-2280.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Enhanced astaxanthin extraction efficiency from Haematococcus pluvialis via the cyst germination in outdoor culture systems
AU - Choi, Yoon Young
AU - Hong, Min Eui
AU - Sim, Sang Jun
PY - 2015/12/1
Y1 - 2015/12/1
N2 - Haematococcus pluvialis is the richest source of natural astaxanthin (3S, 3′S), but the rigid cell wall of mature red cyst (aplanospore) complicates the efficient extraction of astaxanthin from the strain. Herein, the cyst germination method was developed by using nitrate and light for practical application for an efficient astaxanthin extraction from Haematococcus cells cultured in outdoor condition where flue gas, solar radiation and photobioreactor were used. Notably, autotrophic germination rate was easily regulated by light intensity. Under conditions of low germination rate, total astaxanthin concentration was highly maintained and astaxanthin extraction efficiency was rapidly enhanced during autotrophic germination. As a result, under homogenization (30 s), the extracted astaxanthin concentration in the cells treated with 1 mM KNO3-150 μE/m2/s was highly increased by 58% compared to the cells treated without germination. Our technical solution will definitely improve an astaxanthin extraction titer with the practical application in outdoor Haematococcus culture system.
AB - Haematococcus pluvialis is the richest source of natural astaxanthin (3S, 3′S), but the rigid cell wall of mature red cyst (aplanospore) complicates the efficient extraction of astaxanthin from the strain. Herein, the cyst germination method was developed by using nitrate and light for practical application for an efficient astaxanthin extraction from Haematococcus cells cultured in outdoor condition where flue gas, solar radiation and photobioreactor were used. Notably, autotrophic germination rate was easily regulated by light intensity. Under conditions of low germination rate, total astaxanthin concentration was highly maintained and astaxanthin extraction efficiency was rapidly enhanced during autotrophic germination. As a result, under homogenization (30 s), the extracted astaxanthin concentration in the cells treated with 1 mM KNO3-150 μE/m2/s was highly increased by 58% compared to the cells treated without germination. Our technical solution will definitely improve an astaxanthin extraction titer with the practical application in outdoor Haematococcus culture system.
KW - Astaxanthin
KW - Cyst germination
KW - Haematococcus pluvialis
KW - Nitrate and solar radiation
KW - Outdoor autotrophic culture
UR - http://www.scopus.com/inward/record.url?scp=84949009490&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84949009490&partnerID=8YFLogxK
U2 - 10.1016/j.procbio.2015.09.008
DO - 10.1016/j.procbio.2015.09.008
M3 - Article
AN - SCOPUS:84949009490
VL - 50
SP - 2275
EP - 2280
JO - Process Biochemistry
JF - Process Biochemistry
SN - 1359-5113
IS - 12
ER -