TY - JOUR
T1 - Enhanced astaxanthin extraction efficiency from Haematococcus pluvialis via the cyst germination in outdoor culture systems
AU - Choi, Yoon Young
AU - Hong, Min Eui
AU - Sim, Sang Jun
N1 - Funding Information:
This work was supported by the “Energy Efficiency & Resources Technology R&D” project (grant no. 20152010201900) of the Korea Institute of Energy Technology Evaluation and Planning (KETEP) grant funded by the Korea government Ministry of Trade, Industry & Energy (MOTIE) and supported by the National Research Foundation (NRF) grants (grant no. NRF-2013R1A2A1A01015644/2010-0027955), University-Institute Cooperation Program (2013) and also supported by the Korea CCS R&D Center (KCRC) grant (grant no. 2014M1A8A1049278), funded by the Korean Government Ministry of Science, ICT & Future Planning (MSIP). Special thanks are given to DrJaoon Young Hwan Kim for early discussions about this work which highly contributed to it and inspired its main idea.
Publisher Copyright:
© 2015 Elsevier Ltd.
Copyright:
Copyright 2015 Elsevier B.V., All rights reserved.
PY - 2015/12
Y1 - 2015/12
N2 - Haematococcus pluvialis is the richest source of natural astaxanthin (3S, 3′S), but the rigid cell wall of mature red cyst (aplanospore) complicates the efficient extraction of astaxanthin from the strain. Herein, the cyst germination method was developed by using nitrate and light for practical application for an efficient astaxanthin extraction from Haematococcus cells cultured in outdoor condition where flue gas, solar radiation and photobioreactor were used. Notably, autotrophic germination rate was easily regulated by light intensity. Under conditions of low germination rate, total astaxanthin concentration was highly maintained and astaxanthin extraction efficiency was rapidly enhanced during autotrophic germination. As a result, under homogenization (30 s), the extracted astaxanthin concentration in the cells treated with 1 mM KNO3-150 μE/m2/s was highly increased by 58% compared to the cells treated without germination. Our technical solution will definitely improve an astaxanthin extraction titer with the practical application in outdoor Haematococcus culture system.
AB - Haematococcus pluvialis is the richest source of natural astaxanthin (3S, 3′S), but the rigid cell wall of mature red cyst (aplanospore) complicates the efficient extraction of astaxanthin from the strain. Herein, the cyst germination method was developed by using nitrate and light for practical application for an efficient astaxanthin extraction from Haematococcus cells cultured in outdoor condition where flue gas, solar radiation and photobioreactor were used. Notably, autotrophic germination rate was easily regulated by light intensity. Under conditions of low germination rate, total astaxanthin concentration was highly maintained and astaxanthin extraction efficiency was rapidly enhanced during autotrophic germination. As a result, under homogenization (30 s), the extracted astaxanthin concentration in the cells treated with 1 mM KNO3-150 μE/m2/s was highly increased by 58% compared to the cells treated without germination. Our technical solution will definitely improve an astaxanthin extraction titer with the practical application in outdoor Haematococcus culture system.
KW - Astaxanthin
KW - Cyst germination
KW - Haematococcus pluvialis
KW - Nitrate and solar radiation
KW - Outdoor autotrophic culture
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U2 - 10.1016/j.procbio.2015.09.008
DO - 10.1016/j.procbio.2015.09.008
M3 - Article
AN - SCOPUS:84949009490
VL - 50
SP - 2275
EP - 2280
JO - Process Biochemistry
JF - Process Biochemistry
SN - 1359-5113
IS - 12
ER -