Abstract
For efficient astaxanthin production from the culture of green microalga, Haematococcus pluvialis, a two-stage mixotrophic culture system was established with stepwise increased light irradiance. By perfusion process, high density biomass (2.47 g/L) was achieved during the vegetative stage due to no detrimental effect of inhibitory metabolites, which was 3.09 and 1.67 times higher than batch and fed-batch processes, respectively. During the induction stage, biomass and astaxanthin were subsequently produced to the very high level 12.3 g/L and 602 mg/L, under stepwise increased light irradiance (150-450 μE/m(2)/s), respectively. These results indicate that the combinatorial approach of perfusion culture during the vegetative stage and stepwise light irradiation during the induction stage is a promising strategy for the simultaneous production of high concentration of biomass and astaxanthin in microalgae including H. pluvialis.
| Original language | English |
|---|---|
| Pages (from-to) | 2039-2047 |
| Number of pages | 9 |
| Journal | Bioprocess and Biosystems Engineering |
| Volume | 37 |
| Issue number | 10 |
| DOIs | |
| Publication status | Published - 2014 Oct 1 |
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ASJC Scopus subject areas
- Medicine(all)
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Enhanced astaxanthin production from microalga, Haematococcus pluvialis by two-stage perfusion culture with stepwise light irradiation. / Park, Joon C hul; Choi, Seung P hill; Hong, Min Eui; Sim, Sang Jun.
In: Bioprocess and Biosystems Engineering, Vol. 37, No. 10, 01.10.2014, p. 2039-2047.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Enhanced astaxanthin production from microalga, Haematococcus pluvialis by two-stage perfusion culture with stepwise light irradiation
AU - Park, Joon C hul
AU - Choi, Seung P hill
AU - Hong, Min Eui
AU - Sim, Sang Jun
PY - 2014/10/1
Y1 - 2014/10/1
N2 - For efficient astaxanthin production from the culture of green microalga, Haematococcus pluvialis, a two-stage mixotrophic culture system was established with stepwise increased light irradiance. By perfusion process, high density biomass (2.47 g/L) was achieved during the vegetative stage due to no detrimental effect of inhibitory metabolites, which was 3.09 and 1.67 times higher than batch and fed-batch processes, respectively. During the induction stage, biomass and astaxanthin were subsequently produced to the very high level 12.3 g/L and 602 mg/L, under stepwise increased light irradiance (150-450 μE/m(2)/s), respectively. These results indicate that the combinatorial approach of perfusion culture during the vegetative stage and stepwise light irradiation during the induction stage is a promising strategy for the simultaneous production of high concentration of biomass and astaxanthin in microalgae including H. pluvialis.
AB - For efficient astaxanthin production from the culture of green microalga, Haematococcus pluvialis, a two-stage mixotrophic culture system was established with stepwise increased light irradiance. By perfusion process, high density biomass (2.47 g/L) was achieved during the vegetative stage due to no detrimental effect of inhibitory metabolites, which was 3.09 and 1.67 times higher than batch and fed-batch processes, respectively. During the induction stage, biomass and astaxanthin were subsequently produced to the very high level 12.3 g/L and 602 mg/L, under stepwise increased light irradiance (150-450 μE/m(2)/s), respectively. These results indicate that the combinatorial approach of perfusion culture during the vegetative stage and stepwise light irradiation during the induction stage is a promising strategy for the simultaneous production of high concentration of biomass and astaxanthin in microalgae including H. pluvialis.
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UR - http://www.scopus.com/inward/citedby.url?scp=84930694266&partnerID=8YFLogxK
U2 - 10.1007/s00449-014-1180-y
DO - 10.1007/s00449-014-1180-y
M3 - Article
C2 - 24700132
AN - SCOPUS:84930694266
VL - 37
SP - 2039
EP - 2047
JO - Bioprocess and Biosystems Engineering
JF - Bioprocess and Biosystems Engineering
SN - 1615-7591
IS - 10
ER -