Enhanced astaxanthin production from microalga, Haematococcus pluvialis by two-stage perfusion culture with stepwise light irradiation

Joon Chul Park, Seung Phill Choi, Min Eui Hong, Sang Jun Sim

Research output: Contribution to journalArticlepeer-review

40 Citations (Scopus)

Abstract

For efficient astaxanthin production from the culture of green microalga, Haematococcus pluvialis, a two-stage mixotrophic culture system was established with stepwise increased light irradiance. By perfusion process, high density biomass (2.47 g/L) was achieved during the vegetative stage due to no detrimental effect of inhibitory metabolites, which was 3.09 and 1.67 times higher than batch and fed-batch processes, respectively. During the induction stage, biomass and astaxanthin were subsequently produced to the very high level 12.3 g/L and 602 mg/L, under stepwise increased light irradiance (150-450 μE/m2/s), respectively. These results indicate that the combinatorial approach of perfusion culture during the vegetative stage and stepwise light irradiation during the induction stage is a promising strategy for the simultaneous production of high concentration of biomass and astaxanthin in microalgae including H. pluvialis.

Original languageEnglish
Pages (from-to)2039-2047
Number of pages9
JournalBioprocess and Biosystems Engineering
Volume37
Issue number10
DOIs
Publication statusPublished - 2014 Sep 12

Keywords

  • Astaxanthin
  • Haematococcus pluvialis
  • Microalgae
  • Mixotrophic culture
  • Perfusion
  • Stepwise irradiation

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering

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