Enhanced autotrophic astaxanthin production from Haematococcus pluvialis under high temperature via heat stress-driven Haber–Weiss reaction

Min Eui Hong, Sung Kwan Hwang, Won Seok Chang, Byung Woo Kim, Jeewon Lee, Sang Jun Sim

Research output: Contribution to journalArticle

39 Citations (Scopus)

Abstract

High temperatures (30–36 °C) inhibited astaxanthin accumulation in Haematococcus pluvialis under photoautotrophic conditions. The depression of carotenogenesis was primarily attributed to excess intracellular less reactive oxygen species (LROS; O<inf>2</inf><sup>−</sup> and H<inf>2</inf>O<inf>2</inf>) levels generated under high temperature conditions. Here, we show that the heat stress-driven inefficient astaxanthin production was improved by accelerating the iron-catalyzed Haber–Weiss reaction to convert LROS into more reactive oxygen species (MROS; O<inf>2</inf> and OH·), thereby facilitating lipid peroxidation. As a result, during 18 days of photoautotrophic induction, the astaxanthin concentration of cells cultured in high temperatures in the presence of iron (450 μM) was dramatically increased by 75 % (30 °C) and 133 % (36 °C) compared to that of cells exposed to heat stress alone. The heat stress-driven Haber–Weiss reaction will be useful for economically producing astaxanthin by reducing energy cost and enhancing photoautotrophic astaxanthin production, particularly outdoors utilizing natural solar radiation including heat and light for photo-induction of H. pluvialis.

Original languageEnglish
Pages (from-to)5203-5215
Number of pages13
JournalApplied Microbiology and Biotechnology
Volume99
Issue number12
DOIs
Publication statusPublished - 2015 Feb 17

Keywords

  • Astaxanthin
  • Haber–Weiss reaction
  • Haematococcus pluvialis
  • High temperature
  • Photoautotrophic induction

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology

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