Enhanced biodiesel production in Neochloris oleoabundans by a semi-continuous process in two-stage photobioreactors

Se Young Yoon, Min Eui Hong, Won Seok Chang, Sang Jun Sim

Research output: Contribution to journalArticlepeer-review

24 Citations (Scopus)


Under autotrophic conditions, highly productive biodiesel production was achieved using a semi-continuous culture system in Neochloris oleoabundans. In particular, the flue gas generated by combustion of liquefied natural gas and natural solar radiation was used for cost-effective microalgal culture system. In semi-continuous culture, the greater part (∼80 %) of the culture volume containing vegetative cells grown under nitrogen-replete conditions in a first photobioreactor (PBR) was directly transferred to a second PBR and cultured sequentially under nitrogen-depleted conditions for accelerating oil accumulation. As a result, in semi-continuous culture, the productivities of biomass and biodiesel in the cells were increased by 58 % (growth phase) and 51 % (induction phase) compared to the cells in batch culture, respectively. The semi-continuous culture system using two stage photobioreactors is a very efficient strategy to further improve biodiesel production from microalgae under photoautotrophic conditions.

Original languageEnglish
Pages (from-to)1415-1421
Number of pages7
JournalBioprocess and Biosystems Engineering
Issue number7
Publication statusPublished - 2015 Jul 29


  • Closed-photobioreactor
  • Flue gas
  • Natural sunlight
  • Neochloris oleoabundans
  • Photoautotrophic biodiesel production
  • Semi-continuous process

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering


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