Enhanced blood compatibility of polymers grafted by sulfonated PEO via a negative cilia concept

Young Ha Kim, Dong Keun Han, Ki Dong Park, Soo Hyun Kim

Research output: Contribution to journalArticle

91 Citations (Scopus)

Abstract

In our laboratory sulfonated PEO (PEO-SO3) was designed as a "negative cilia model" to investigate a synergistic effect of PEO and negatively charged SO3 groups. PEO-SO3 itself exhibited a heparin-like anticoagulant activity of 14% of free heparin. Polyurethane grafted with PEO-SO3 (PU-PEO-SO3) increased the albumin adsorption to a great extent but suppressed other proteins, while PU-PEO decreased the adsorption of all the proteins. The platelet adhesion was decreased on PU-PEO but least on PU-PEO-SO3 to demonstrate an additional effect of SO3 groups. The enhanced blood compatibility of PU-PEO-SO3 in the ex vivo rabbit and in vivo canine implanting tests was confirmed. Furthermore, PU-PEO-SO3 exhibited an improved biostability and suppressed calcification in addition to the enhanced antithrombogenicity. The in vivo antithrombogenicity and biostability were improved in the order of PU<PU-PEO<PU-PEO-SO3. The calcium amounts deposited was decreased in the order of PU>PU-PEO>PU-PEO-SO3 in spite of the possible attraction between negative SO3 groups and positive calcium ions. The bioprosthetic tissue (BT) was grafted with H2N-PEO-SO3 via glutaraldehyde (GA) residues after conventional GA fixation. BT-PEO-SO3 also displayed the decreased calcification by in vivo animal models. The application of PEO-SO3 was extended by designing amphiphilic copolymers containing PEO-SO3 moiety and hydrophobic long alkyl groups as anchors. The superior effect of PEO-SO3 groups on thromboresistance compared to PEO was confirmed also in the case of copolymers coated or blended with other polymers and the systems coupled by UV irradiation, photoreaction or gold/sulfur or silane coupling technology, and therefore it might be very useful for the medical devices.

Original languageEnglish
Pages (from-to)2213-2223
Number of pages11
JournalBiomaterials
Volume24
Issue number13
DOIs
Publication statusPublished - 2003 Jun 1
Externally publishedYes

Fingerprint

Cilia
Polyethylene oxides
Polymers
Blood
Polyurethanes
Glutaral
amino-polyethyleneoxide-sulfonate
Adsorption
Heparin
Copolymers
Tissue
Silanes
Platelets
Anchors
Sulfur
Proteins
Gold
Anticoagulants
Canidae
Albumins

Keywords

  • Biostability
  • Calcification
  • Modified tissue
  • Sulfonated PEO grafting
  • Thromboresistance

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Biomedical Engineering

Cite this

Enhanced blood compatibility of polymers grafted by sulfonated PEO via a negative cilia concept. / Kim, Young Ha; Han, Dong Keun; Park, Ki Dong; Kim, Soo Hyun.

In: Biomaterials, Vol. 24, No. 13, 01.06.2003, p. 2213-2223.

Research output: Contribution to journalArticle

Kim, Young Ha ; Han, Dong Keun ; Park, Ki Dong ; Kim, Soo Hyun. / Enhanced blood compatibility of polymers grafted by sulfonated PEO via a negative cilia concept. In: Biomaterials. 2003 ; Vol. 24, No. 13. pp. 2213-2223.
@article{4450a15e9d944833a7a2c54f10621de2,
title = "Enhanced blood compatibility of polymers grafted by sulfonated PEO via a negative cilia concept",
abstract = "In our laboratory sulfonated PEO (PEO-SO3) was designed as a {"}negative cilia model{"} to investigate a synergistic effect of PEO and negatively charged SO3 groups. PEO-SO3 itself exhibited a heparin-like anticoagulant activity of 14{\%} of free heparin. Polyurethane grafted with PEO-SO3 (PU-PEO-SO3) increased the albumin adsorption to a great extent but suppressed other proteins, while PU-PEO decreased the adsorption of all the proteins. The platelet adhesion was decreased on PU-PEO but least on PU-PEO-SO3 to demonstrate an additional effect of SO3 groups. The enhanced blood compatibility of PU-PEO-SO3 in the ex vivo rabbit and in vivo canine implanting tests was confirmed. Furthermore, PU-PEO-SO3 exhibited an improved biostability and suppressed calcification in addition to the enhanced antithrombogenicity. The in vivo antithrombogenicity and biostability were improved in the order of PU<PU-PEO<PU-PEO-SO3. The calcium amounts deposited was decreased in the order of PU>PU-PEO>PU-PEO-SO3 in spite of the possible attraction between negative SO3 groups and positive calcium ions. The bioprosthetic tissue (BT) was grafted with H2N-PEO-SO3 via glutaraldehyde (GA) residues after conventional GA fixation. BT-PEO-SO3 also displayed the decreased calcification by in vivo animal models. The application of PEO-SO3 was extended by designing amphiphilic copolymers containing PEO-SO3 moiety and hydrophobic long alkyl groups as anchors. The superior effect of PEO-SO3 groups on thromboresistance compared to PEO was confirmed also in the case of copolymers coated or blended with other polymers and the systems coupled by UV irradiation, photoreaction or gold/sulfur or silane coupling technology, and therefore it might be very useful for the medical devices.",
keywords = "Biostability, Calcification, Modified tissue, Sulfonated PEO grafting, Thromboresistance",
author = "Kim, {Young Ha} and Han, {Dong Keun} and Park, {Ki Dong} and Kim, {Soo Hyun}",
year = "2003",
month = "6",
day = "1",
doi = "10.1016/S0142-9612(03)00023-1",
language = "English",
volume = "24",
pages = "2213--2223",
journal = "Biomaterials",
issn = "0142-9612",
publisher = "Elsevier BV",
number = "13",

}

TY - JOUR

T1 - Enhanced blood compatibility of polymers grafted by sulfonated PEO via a negative cilia concept

AU - Kim, Young Ha

AU - Han, Dong Keun

AU - Park, Ki Dong

AU - Kim, Soo Hyun

PY - 2003/6/1

Y1 - 2003/6/1

N2 - In our laboratory sulfonated PEO (PEO-SO3) was designed as a "negative cilia model" to investigate a synergistic effect of PEO and negatively charged SO3 groups. PEO-SO3 itself exhibited a heparin-like anticoagulant activity of 14% of free heparin. Polyurethane grafted with PEO-SO3 (PU-PEO-SO3) increased the albumin adsorption to a great extent but suppressed other proteins, while PU-PEO decreased the adsorption of all the proteins. The platelet adhesion was decreased on PU-PEO but least on PU-PEO-SO3 to demonstrate an additional effect of SO3 groups. The enhanced blood compatibility of PU-PEO-SO3 in the ex vivo rabbit and in vivo canine implanting tests was confirmed. Furthermore, PU-PEO-SO3 exhibited an improved biostability and suppressed calcification in addition to the enhanced antithrombogenicity. The in vivo antithrombogenicity and biostability were improved in the order of PU<PU-PEO<PU-PEO-SO3. The calcium amounts deposited was decreased in the order of PU>PU-PEO>PU-PEO-SO3 in spite of the possible attraction between negative SO3 groups and positive calcium ions. The bioprosthetic tissue (BT) was grafted with H2N-PEO-SO3 via glutaraldehyde (GA) residues after conventional GA fixation. BT-PEO-SO3 also displayed the decreased calcification by in vivo animal models. The application of PEO-SO3 was extended by designing amphiphilic copolymers containing PEO-SO3 moiety and hydrophobic long alkyl groups as anchors. The superior effect of PEO-SO3 groups on thromboresistance compared to PEO was confirmed also in the case of copolymers coated or blended with other polymers and the systems coupled by UV irradiation, photoreaction or gold/sulfur or silane coupling technology, and therefore it might be very useful for the medical devices.

AB - In our laboratory sulfonated PEO (PEO-SO3) was designed as a "negative cilia model" to investigate a synergistic effect of PEO and negatively charged SO3 groups. PEO-SO3 itself exhibited a heparin-like anticoagulant activity of 14% of free heparin. Polyurethane grafted with PEO-SO3 (PU-PEO-SO3) increased the albumin adsorption to a great extent but suppressed other proteins, while PU-PEO decreased the adsorption of all the proteins. The platelet adhesion was decreased on PU-PEO but least on PU-PEO-SO3 to demonstrate an additional effect of SO3 groups. The enhanced blood compatibility of PU-PEO-SO3 in the ex vivo rabbit and in vivo canine implanting tests was confirmed. Furthermore, PU-PEO-SO3 exhibited an improved biostability and suppressed calcification in addition to the enhanced antithrombogenicity. The in vivo antithrombogenicity and biostability were improved in the order of PU<PU-PEO<PU-PEO-SO3. The calcium amounts deposited was decreased in the order of PU>PU-PEO>PU-PEO-SO3 in spite of the possible attraction between negative SO3 groups and positive calcium ions. The bioprosthetic tissue (BT) was grafted with H2N-PEO-SO3 via glutaraldehyde (GA) residues after conventional GA fixation. BT-PEO-SO3 also displayed the decreased calcification by in vivo animal models. The application of PEO-SO3 was extended by designing amphiphilic copolymers containing PEO-SO3 moiety and hydrophobic long alkyl groups as anchors. The superior effect of PEO-SO3 groups on thromboresistance compared to PEO was confirmed also in the case of copolymers coated or blended with other polymers and the systems coupled by UV irradiation, photoreaction or gold/sulfur or silane coupling technology, and therefore it might be very useful for the medical devices.

KW - Biostability

KW - Calcification

KW - Modified tissue

KW - Sulfonated PEO grafting

KW - Thromboresistance

UR - http://www.scopus.com/inward/record.url?scp=0037409734&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0037409734&partnerID=8YFLogxK

U2 - 10.1016/S0142-9612(03)00023-1

DO - 10.1016/S0142-9612(03)00023-1

M3 - Article

C2 - 12699657

AN - SCOPUS:0037409734

VL - 24

SP - 2213

EP - 2223

JO - Biomaterials

JF - Biomaterials

SN - 0142-9612

IS - 13

ER -