Enhanced butanol fermentation using metabolically engineered Clostridium acetobutylicum with ex situ recovery of butanol

Sang Hyun Lee, Sooah Kim, Jung Yeon Kim, Nam Yong Cheong, Kyoung Heon Kim

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

In this study, metabolic target reactions for strain engineering were searched via intracellular coenzyme A (CoA) metabolite analysis. The metabolic reactions catalyzed by thiolase (AtoB) and aldehyde-alcohol dehydrogenase (AdhE1) were considered potential rate-limiting steps. In addition, CoA transferase (CtfAB) was highlighted as being important for the assimilation of organic acids, in order to achieve high butanol production. Based on this quantitative analysis, the BEKW_E1AB-atoB strain was constructed by overexpressing the thl (atoB), adhE1, and ctfAB genes in Clostridium acetobutylicum strain BEKW, which has the phosphotransacetylase (pta) and butyrate kinase (buk) genes knocked out. After 100 h of continuous fermentation coupled with adsorptive ex situ butanol recovery, the concentrations found after considering desorption, yield, and productivity for the BEKW_E1AB-atoB strain were 55.7 g/L, 0.38 g/g, and 2.64 g/L/h, respectively. The level of butanol production achieved (2.64 g/L/h) represents the highest reported value obtained after adsorptive, long-term fermentation.

Original languageEnglish
Pages (from-to)909-917
Number of pages9
JournalBioresource Technology
Volume218
DOIs
Publication statusPublished - 2016 Oct 1

Keywords

  • Acetone-butanol-ethanol fermentation
  • Clostridium acetobutylicum
  • Coenzyme A
  • Intracellular metabolite analysis
  • Metabolic engineering

ASJC Scopus subject areas

  • Bioengineering
  • Environmental Engineering
  • Waste Management and Disposal

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