Abstract
In this study, metabolic target reactions for strain engineering were searched via intracellular coenzyme A (CoA) metabolite analysis. The metabolic reactions catalyzed by thiolase (AtoB) and aldehyde-alcohol dehydrogenase (AdhE1) were considered potential rate-limiting steps. In addition, CoA transferase (CtfAB) was highlighted as being important for the assimilation of organic acids, in order to achieve high butanol production. Based on this quantitative analysis, the BEKW_E1AB-atoB strain was constructed by overexpressing the thl (atoB), adhE1, and ctfAB genes in Clostridium acetobutylicum strain BEKW, which has the phosphotransacetylase (pta) and butyrate kinase (buk) genes knocked out. After 100 h of continuous fermentation coupled with adsorptive ex situ butanol recovery, the concentrations found after considering desorption, yield, and productivity for the BEKW_E1AB-atoB strain were 55.7 g/L, 0.38 g/g, and 2.64 g/L/h, respectively. The level of butanol production achieved (2.64 g/L/h) represents the highest reported value obtained after adsorptive, long-term fermentation.
| Original language | English |
|---|---|
| Pages (from-to) | 909-917 |
| Number of pages | 9 |
| Journal | Bioresource Technology |
| Volume | 218 |
| DOIs | |
| Publication status | Published - 2016 Oct 1 |
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Keywords
- Acetone-butanol-ethanol fermentation
- Clostridium acetobutylicum
- Coenzyme A
- Intracellular metabolite analysis
- Metabolic engineering
ASJC Scopus subject areas
- Bioengineering
- Environmental Engineering
- Waste Management and Disposal
Cite this
Enhanced butanol fermentation using metabolically engineered Clostridium acetobutylicum with ex situ recovery of butanol. / Lee, Sang Hyun; Kim, Sooah; Kim, Jung Yeon; Cheong, Nam Yong; Kim, Kyoung Heon.
In: Bioresource Technology, Vol. 218, 01.10.2016, p. 909-917.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Enhanced butanol fermentation using metabolically engineered Clostridium acetobutylicum with ex situ recovery of butanol
AU - Lee, Sang Hyun
AU - Kim, Sooah
AU - Kim, Jung Yeon
AU - Cheong, Nam Yong
AU - Kim, Kyoung Heon
PY - 2016/10/1
Y1 - 2016/10/1
N2 - In this study, metabolic target reactions for strain engineering were searched via intracellular coenzyme A (CoA) metabolite analysis. The metabolic reactions catalyzed by thiolase (AtoB) and aldehyde-alcohol dehydrogenase (AdhE1) were considered potential rate-limiting steps. In addition, CoA transferase (CtfAB) was highlighted as being important for the assimilation of organic acids, in order to achieve high butanol production. Based on this quantitative analysis, the BEKW_E1AB-atoB strain was constructed by overexpressing the thl (atoB), adhE1, and ctfAB genes in Clostridium acetobutylicum strain BEKW, which has the phosphotransacetylase (pta) and butyrate kinase (buk) genes knocked out. After 100 h of continuous fermentation coupled with adsorptive ex situ butanol recovery, the concentrations found after considering desorption, yield, and productivity for the BEKW_E1AB-atoB strain were 55.7 g/L, 0.38 g/g, and 2.64 g/L/h, respectively. The level of butanol production achieved (2.64 g/L/h) represents the highest reported value obtained after adsorptive, long-term fermentation.
AB - In this study, metabolic target reactions for strain engineering were searched via intracellular coenzyme A (CoA) metabolite analysis. The metabolic reactions catalyzed by thiolase (AtoB) and aldehyde-alcohol dehydrogenase (AdhE1) were considered potential rate-limiting steps. In addition, CoA transferase (CtfAB) was highlighted as being important for the assimilation of organic acids, in order to achieve high butanol production. Based on this quantitative analysis, the BEKW_E1AB-atoB strain was constructed by overexpressing the thl (atoB), adhE1, and ctfAB genes in Clostridium acetobutylicum strain BEKW, which has the phosphotransacetylase (pta) and butyrate kinase (buk) genes knocked out. After 100 h of continuous fermentation coupled with adsorptive ex situ butanol recovery, the concentrations found after considering desorption, yield, and productivity for the BEKW_E1AB-atoB strain were 55.7 g/L, 0.38 g/g, and 2.64 g/L/h, respectively. The level of butanol production achieved (2.64 g/L/h) represents the highest reported value obtained after adsorptive, long-term fermentation.
KW - Acetone-butanol-ethanol fermentation
KW - Clostridium acetobutylicum
KW - Coenzyme A
KW - Intracellular metabolite analysis
KW - Metabolic engineering
UR - http://www.scopus.com/inward/record.url?scp=84978370478&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84978370478&partnerID=8YFLogxK
U2 - 10.1016/j.biortech.2016.07.060
DO - 10.1016/j.biortech.2016.07.060
M3 - Article
C2 - 27441828
AN - SCOPUS:84978370478
VL - 218
SP - 909
EP - 917
JO - Bioresource Technology
JF - Bioresource Technology
SN - 0960-8524
ER -