TY - JOUR
T1 - Enhanced butanol fermentation using metabolically engineered Clostridium acetobutylicum with ex situ recovery of butanol
AU - Lee, Sang Hyun
AU - Kim, Sooah
AU - Kim, Jung Yeon
AU - Cheong, Nam Yong
AU - Kim, Kyoung Heon
N1 - Funding Information:
The authors are grateful to Professor Sang Yup Lee from the Korea Advanced Institute of Science and Technology for kindly providing the BEKW strain and Min-A Kwon for providing technical assistance. KHK acknowledges the facility support of the Institute of Biomedical and Food Safety at CJ Food Safety Hall, Korea University.
PY - 2016/10/1
Y1 - 2016/10/1
N2 - In this study, metabolic target reactions for strain engineering were searched via intracellular coenzyme A (CoA) metabolite analysis. The metabolic reactions catalyzed by thiolase (AtoB) and aldehyde-alcohol dehydrogenase (AdhE1) were considered potential rate-limiting steps. In addition, CoA transferase (CtfAB) was highlighted as being important for the assimilation of organic acids, in order to achieve high butanol production. Based on this quantitative analysis, the BEKW_E1AB-atoB strain was constructed by overexpressing the thl (atoB), adhE1, and ctfAB genes in Clostridium acetobutylicum strain BEKW, which has the phosphotransacetylase (pta) and butyrate kinase (buk) genes knocked out. After 100 h of continuous fermentation coupled with adsorptive ex situ butanol recovery, the concentrations found after considering desorption, yield, and productivity for the BEKW_E1AB-atoB strain were 55.7 g/L, 0.38 g/g, and 2.64 g/L/h, respectively. The level of butanol production achieved (2.64 g/L/h) represents the highest reported value obtained after adsorptive, long-term fermentation.
AB - In this study, metabolic target reactions for strain engineering were searched via intracellular coenzyme A (CoA) metabolite analysis. The metabolic reactions catalyzed by thiolase (AtoB) and aldehyde-alcohol dehydrogenase (AdhE1) were considered potential rate-limiting steps. In addition, CoA transferase (CtfAB) was highlighted as being important for the assimilation of organic acids, in order to achieve high butanol production. Based on this quantitative analysis, the BEKW_E1AB-atoB strain was constructed by overexpressing the thl (atoB), adhE1, and ctfAB genes in Clostridium acetobutylicum strain BEKW, which has the phosphotransacetylase (pta) and butyrate kinase (buk) genes knocked out. After 100 h of continuous fermentation coupled with adsorptive ex situ butanol recovery, the concentrations found after considering desorption, yield, and productivity for the BEKW_E1AB-atoB strain were 55.7 g/L, 0.38 g/g, and 2.64 g/L/h, respectively. The level of butanol production achieved (2.64 g/L/h) represents the highest reported value obtained after adsorptive, long-term fermentation.
KW - Acetone-butanol-ethanol fermentation
KW - Clostridium acetobutylicum
KW - Coenzyme A
KW - Intracellular metabolite analysis
KW - Metabolic engineering
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U2 - 10.1016/j.biortech.2016.07.060
DO - 10.1016/j.biortech.2016.07.060
M3 - Article
C2 - 27441828
AN - SCOPUS:84978370478
VL - 218
SP - 909
EP - 917
JO - Bioresource Technology
JF - Bioresource Technology
SN - 0960-8524
ER -
