Enhanced calcium carbonate-biofilm complex formation by alkali-generating Lysinibacillus boronitolerans YS11 and alkaliphilic Bacillus sp. AK13

Yun Suk Lee, Woojun Park

Research output: Contribution to journalArticle

Abstract

Microbially induced calcium carbonate (CaCO 3 ) precipitation (MICP) is a process where microbes induce condition favorable for CaCO 3 formation through metabolic activities by increasing the pH or carbonate ions when calcium is near. The molecular and ecological basis of CaCO 3 precipitating (CCP) bacteria has been poorly illuminated. Here, we showed that increased pH levels by deamination of amino acids is a driving force toward MICP using alkalitolerant Lysinibacillus boronitolerans YS11 as a model species of non-ureolytic CCP bacteria. This alkaline generation also facilitates the growth of neighboring alkaliphilic Bacillus sp. AK13, which could alter characteristics of MICP by changing the size and shape of CaCO 3 minerals. Furthermore, we showed CaCO 3 that precipitates earlier in an experiment modifies membrane rigidity of YS11 strain via upregulation of branched chain fatty acid synthesis. This work closely examines MICP conditions by deamination and the effect of MICP on cell membrane rigidity and crystal formation for the first time.

Original languageEnglish
Article number49
JournalAMB Express
Volume9
Issue number1
DOIs
Publication statusPublished - 2019 Dec 1

Fingerprint

Deamination
Calcium Carbonate
Alkalies
Biofilms
Bacillus
Bacteria
Carbonates
Minerals
Up-Regulation
Fatty Acids
Cell Membrane
Ions
Calcium
Amino Acids
Membranes
Growth

Keywords

  • Alkaline generation
  • Bacteria-CaCO interaction
  • Branched chain fatty acid synthesis
  • Dual species CaCO precipitation
  • Membrane rigidity

ASJC Scopus subject areas

  • Biophysics
  • Applied Microbiology and Biotechnology

Cite this

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title = "Enhanced calcium carbonate-biofilm complex formation by alkali-generating Lysinibacillus boronitolerans YS11 and alkaliphilic Bacillus sp. AK13",
abstract = "Microbially induced calcium carbonate (CaCO 3 ) precipitation (MICP) is a process where microbes induce condition favorable for CaCO 3 formation through metabolic activities by increasing the pH or carbonate ions when calcium is near. The molecular and ecological basis of CaCO 3 precipitating (CCP) bacteria has been poorly illuminated. Here, we showed that increased pH levels by deamination of amino acids is a driving force toward MICP using alkalitolerant Lysinibacillus boronitolerans YS11 as a model species of non-ureolytic CCP bacteria. This alkaline generation also facilitates the growth of neighboring alkaliphilic Bacillus sp. AK13, which could alter characteristics of MICP by changing the size and shape of CaCO 3 minerals. Furthermore, we showed CaCO 3 that precipitates earlier in an experiment modifies membrane rigidity of YS11 strain via upregulation of branched chain fatty acid synthesis. This work closely examines MICP conditions by deamination and the effect of MICP on cell membrane rigidity and crystal formation for the first time.",
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N2 - Microbially induced calcium carbonate (CaCO 3 ) precipitation (MICP) is a process where microbes induce condition favorable for CaCO 3 formation through metabolic activities by increasing the pH or carbonate ions when calcium is near. The molecular and ecological basis of CaCO 3 precipitating (CCP) bacteria has been poorly illuminated. Here, we showed that increased pH levels by deamination of amino acids is a driving force toward MICP using alkalitolerant Lysinibacillus boronitolerans YS11 as a model species of non-ureolytic CCP bacteria. This alkaline generation also facilitates the growth of neighboring alkaliphilic Bacillus sp. AK13, which could alter characteristics of MICP by changing the size and shape of CaCO 3 minerals. Furthermore, we showed CaCO 3 that precipitates earlier in an experiment modifies membrane rigidity of YS11 strain via upregulation of branched chain fatty acid synthesis. This work closely examines MICP conditions by deamination and the effect of MICP on cell membrane rigidity and crystal formation for the first time.

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