Enhanced cell labeling with electropreconcentration

H. Shirinkami, B. Kim, Honggu Chun

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

This study presents a high efficiency on-chip tumor cell labeling technique for cell-based diagnostics by taking advantage from local enhancement of antibody's concentration. Two different approaches, namely overlapping EDL in nanochannels and negatively charged polymer, have been applied for utilizing ion-selectivity in order to induce preconcentration. It has been found that electro-kinetic insatiability in ion concentration polarization results in microcirculation, which facilitates labeling as a result of larger convective terms. Experiments showed that owing to both locally-increased concentration of antibody and microcirculations required time for labeling dramatically decreased to two minutes compared to 45 minute-long conventional labeling procedure.

Original languageEnglish
Title of host publicationMicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences
PublisherChemical and Biological Microsystems Society
Pages987-989
Number of pages3
ISBN (Electronic)9780979806483
Publication statusPublished - 2015
Event19th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2015 - Gyeongju, Korea, Republic of
Duration: 2015 Oct 252015 Oct 29

Other

Other19th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2015
CountryKorea, Republic of
CityGyeongju
Period15/10/2515/10/29

Fingerprint

Labeling
Microcirculation
Antibodies
Ions
Tumors
Cells
Polarization
Kinetics
Polymers
Experiments

Keywords

  • Cell labeling
  • Ion selectivity
  • Preconcentration

ASJC Scopus subject areas

  • Control and Systems Engineering

Cite this

Shirinkami, H., Kim, B., & Chun, H. (2015). Enhanced cell labeling with electropreconcentration. In MicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences (pp. 987-989). Chemical and Biological Microsystems Society.

Enhanced cell labeling with electropreconcentration. / Shirinkami, H.; Kim, B.; Chun, Honggu.

MicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences. Chemical and Biological Microsystems Society, 2015. p. 987-989.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Shirinkami, H, Kim, B & Chun, H 2015, Enhanced cell labeling with electropreconcentration. in MicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences. Chemical and Biological Microsystems Society, pp. 987-989, 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2015, Gyeongju, Korea, Republic of, 15/10/25.
Shirinkami H, Kim B, Chun H. Enhanced cell labeling with electropreconcentration. In MicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences. Chemical and Biological Microsystems Society. 2015. p. 987-989
Shirinkami, H. ; Kim, B. ; Chun, Honggu. / Enhanced cell labeling with electropreconcentration. MicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences. Chemical and Biological Microsystems Society, 2015. pp. 987-989
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