Enhanced cell labeling with electropreconcentration

H. Shirinkami, B. Kim, Honggu Chun

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

This study presents a high efficiency on-chip tumor cell labeling technique for cell-based diagnostics by taking advantage from local enhancement of antibody's concentration. Two different approaches, namely overlapping EDL in nanochannels and negatively charged polymer, have been applied for utilizing ion-selectivity in order to induce preconcentration. It has been found that electro-kinetic insatiability in ion concentration polarization results in microcirculation, which facilitates labeling as a result of larger convective terms. Experiments showed that owing to both locally-increased concentration of antibody and microcirculations required time for labeling dramatically decreased to two minutes compared to 45 minute-long conventional labeling procedure.

Original languageEnglish
Title of host publicationMicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences
PublisherChemical and Biological Microsystems Society
Pages987-989
Number of pages3
ISBN (Electronic)9780979806483
Publication statusPublished - 2015
Event19th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2015 - Gyeongju, Korea, Republic of
Duration: 2015 Oct 252015 Oct 29

Other

Other19th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2015
CountryKorea, Republic of
CityGyeongju
Period15/10/2515/10/29

Keywords

  • Cell labeling
  • Ion selectivity
  • Preconcentration

ASJC Scopus subject areas

  • Control and Systems Engineering

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  • Cite this

    Shirinkami, H., Kim, B., & Chun, H. (2015). Enhanced cell labeling with electropreconcentration. In MicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences (pp. 987-989). Chemical and Biological Microsystems Society.