Enhanced drought tolerance in Arabidopsis via genetic manipulation aimed at the reduction of glucosamine-induced ROS generation

Seung Hee Chu, Ha na Noh, Sooah Kim, Kyoung Heon Kim, Suk Whan Hong, Hojoung Lee

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

In animals, high glucose exerts some of its deleterious effects by activation of the hexosamine biosynthesis pathway (HBP), a branch of the glycolytic pathway that produces amino sugars (Daniels et al. in Mol Endocrinol 7:1041-1048, 1993; Du et al. in Proc Natl Acad Sci USA 97:12222-12226, 2000). Glucosamine (GlcN) is a naturally occurring amino sugar produced by amidation of fructose-6-phosphate. Previously, we observed that glucosamine (GlcN) inhibits hypocotyl elongation in Arabidopsis thaliana by a process involving the significant increase of reactive oxygen species. The present study investigated the relationship between GlcN-induced ROS generation and abiotic stress responses in Arabidopsis by generating two types of transgenic plant. Scavenging of endogenous GlcN by ectopic expression of E. coli glucosamine-6-phosphate deaminase (NagB) was observed to confer enhanced tolerance to oxidative, drought, and cold stress. Consistent with this result, overproduction of GlcN by the ectopic expression of E. coli glucosamine-6-phosphate synthase (GlmS) induced cell death at an early stage. Taken together, these data suggest that genetic manipulation of endogenous GlcN level can effectively lead to the generation of abiotic stress-tolerant transgenic crop plants.

Original languageEnglish
Pages (from-to)493-502
Number of pages10
JournalPlant Molecular Biology
Volume74
Issue number4
DOIs
Publication statusPublished - 2010 Oct 21

Fingerprint

glucosamine
Droughts
Glucosamine
genetic engineering
Arabidopsis
drought tolerance
Amino Sugars
Genetically Modified Plants
amino sugars
Glutamine-Fructose-6-Phosphate Transaminase (Isomerizing)
abiotic stress
Hexosamines
Hypocotyl
transgenic plants
phosphates
hexosamines
Escherichia coli
fructose 6-phosphate
Reactive Oxygen Species
glycolysis

Keywords

  • Arabidopsis thaliana
  • Drought stress
  • Glms
  • Glucosamine
  • NagB
  • ROS

ASJC Scopus subject areas

  • Plant Science
  • Agronomy and Crop Science
  • Genetics

Cite this

Enhanced drought tolerance in Arabidopsis via genetic manipulation aimed at the reduction of glucosamine-induced ROS generation. / Chu, Seung Hee; Noh, Ha na; Kim, Sooah; Kim, Kyoung Heon; Hong, Suk Whan; Lee, Hojoung.

In: Plant Molecular Biology, Vol. 74, No. 4, 21.10.2010, p. 493-502.

Research output: Contribution to journalArticle

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