Enhanced electrochemical sensitivity of enzyme precipitate coating (EPC)-based glucose oxidase biosensors with increased free CNT loadings

Jae Hyun Kim, Sun Ae Jun, Yongchai Kwon, Su Ha, Byong In Sang, Jungbae Kim

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Enzymatic electrodes were fabricated by using three different immobilizations of glucose oxidase (GOx): covalent enzyme attachment (CA), enzyme coating (EC), and enzyme precipitate coating (EPC), here referred to as CA-E, EC-E, and EPC-E, respectively. When additional carbon nanotubes (CNTs) were introduced from 0 to 75wt% for the EPC-E design, its initial biosensor sensitivity was improved from 2.40×10-3 to 16.26×10-3 AM-1cm-2, while its electron charge transfer rate constant was increased from 0.33 to 1.47s-1. When a fixed ratio of CNTs was added for three different electrode systems, EPC-E showed the best glucose sensitivity and long-term thermal stability. For example, when 75wt% of additional CNTs was added, the initial sensitivity of EPC-E was 16.26×10-3 AM-1cm-2, while those of EC-E and CA-E were only 6.42×10-3 and 1.18×10-3 AM-1cm-2, respectively. Furthermore, EPC-E retained 63% of its initial sensitivity after thermal treatment at 40°C over 41days, while EC-E and CA-E showed only 12% and 1% of initial sensitivities, respectively. Consequently, the EPC approach with additional CNTs achieved both high sensitivity and long-term stability, which are required for continuous and accurate glucose monitoring.

Original languageEnglish
Pages (from-to)114-119
Number of pages6
JournalBioelectrochemistry
Volume101
DOIs
Publication statusPublished - 2015 Feb 1

Fingerprint

Glucose Oxidase
Carbon Nanotubes
Glucose oxidase
oxidase
Biosensing Techniques
bioinstrumentation
Biosensors
glucose
enzymes
Precipitates
precipitates
Carbon nanotubes
Enzymes
carbon nanotubes
coatings
Coatings
sensitivity
attachment
Glucose
Hot Temperature

Keywords

  • Carbon nanotubes
  • Electron generation and transfer
  • Enzymatic glucose sensors
  • Enzyme precipitate coating
  • Glucose oxidase

ASJC Scopus subject areas

  • Biophysics
  • Electrochemistry
  • Physical and Theoretical Chemistry

Cite this

Enhanced electrochemical sensitivity of enzyme precipitate coating (EPC)-based glucose oxidase biosensors with increased free CNT loadings. / Kim, Jae Hyun; Jun, Sun Ae; Kwon, Yongchai; Ha, Su; Sang, Byong In; Kim, Jungbae.

In: Bioelectrochemistry, Vol. 101, 01.02.2015, p. 114-119.

Research output: Contribution to journalArticle

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