Enhanced Electron Transfer Mediated by Conjugated Polyelectrolyte and Its Application to Washing-Free DNA Detection

Seonhwa Park, Ji Eun Jeong, Van Sang Le, Jeongwook Seo, Byeongjun Yu, Da Young Kim, Se Hun Kwon, Sangyong Jon, Han Young Woo, Haesik Yang

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Direct electron transfer between a redox label and an electrode requires a short working distance (<1-2 nm), and in general an affinity biosensor based on direct electron transfer requires a finely smoothed Au electrode to support efficient target binding. Here we report that direct electron transfer over a longer working distance is possible between (i) an anionic π-conjugated polyelectrolyte (CPE) label having many redox-active sites and (ii) a readily prepared, thin polymeric monolayer-modified indium-tin oxide electrode. In addition, the long CPE label (∼18 nm for 10 kDa) can approach the electrode within the working distance after sandwich-type target-specific binding, and fast CPE-mediated oxidation of ammonia borane along the entire CPE backbone affords high signal amplification.

Original languageEnglish
Pages (from-to)2409-2412
Number of pages4
JournalJournal of the American Chemical Society
Volume140
Issue number7
DOIs
Publication statusPublished - 2018 Feb 21

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ASJC Scopus subject areas

  • Catalysis
  • Chemistry(all)
  • Biochemistry
  • Colloid and Surface Chemistry

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