Enhanced enzymatic hydrolysis of hydrothermally pretreated empty fruit bunches at high solids loadings by the synergism of hemicellulase and polyethylene glycol

Jungwoo Yang, Ji Eun Kim, Ha Eun Kim, Ju Hyun Yu, Young Lok Cha, Kyoung Heon Kim

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

In enzymatic hydrolysis, high lignocellulose loadings are required to obtain high sugar titers. However, the high solids loadings limit enzymatic hydrolysis. In this study, to overcome this limitation, the promoting and synergistic effects of the accessory agents of hemicellulase (i.e., Cellic HTec2) and polyethylene glycol (PEG) 8000 were investigated in the enzymatic hydrolysis of hydrothermally pretreated empty fruit bunches (EFBs). After the optimal addition of Cellic HTec2 and PEG, high enzymatic digestion of the pretreated EFBs was achieved owing to their synergistic effects, even at high solids loadings. For example, the enzymatic digestibility of pretreated EFBs at a 21.7% (w/v) solids loading with 10 FPU of Cellic CTec2/g glucan reached 72.5% when 2.7 mg of Cellic HTec2/g glucan and 62.5 mg of PEG/g glucan were used as the accessory agents. These results suggested that the optimal addition of accessory agents is effective for the enhanced hydrolysis of lignocellulose using even a commercial cellulase preparation.

Original languageEnglish
Pages (from-to)211-216
Number of pages6
JournalProcess Biochemistry
Volume58
DOIs
Publication statusPublished - 2017 Jul 1

Keywords

  • Empty fruit bunches
  • Enzymatic hydrolysis
  • High solids loading
  • Hydrothermal pretreatment
  • Saccharification of lignocellulose

ASJC Scopus subject areas

  • Bioengineering
  • Biochemistry
  • Applied Microbiology and Biotechnology

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