Enhanced in-vitro hemozoin polymerization by optimized process using histidine-rich protein II (HRPII)

Ju Hun Lee, Hyeong Ryeol Kim, Ja Hyun Lee, Soo Kweon Lee, Youngsang Chun, Sung Ok Han, Hah Young Yoo, Chulhwan Park, Seung Wook Kim

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Conductive biopolymers, an important class of functional materials, have received attention in various fields because of their unique electrical, optical, and physical properties. In this study, the polymerization of heme into hemozoin was carried out in an in vitro system by the newly developed heme polymerase (histidine-rich protein 2 (HRP-II)). The HRP-II was produced by recombinant E. coli BL21 from the Plasmodium falciparum gene. To improve the hemozoin production, the reaction conditions on the polymerization were investigated and the maximum production was achieved after about 790 μM at 34 °C with 200 rpm for 24 h. As a result, the production was improved about two-fold according to the stepwise optimization in an in vitro system. The produced hemozoin was qualitatively analyzed using the Fourier transform infrared (FTIR) spectroscopy, energy dispersive X-ray spectroscopy (EDS), and scanning electron microscopy (SEM). Finally, it was confirmed that the enzymatically polymerized hemozoin had similar physical properties to chemically synthesized hemozoin. These results could represent a significant potential for nano-biotechnology applications, and also provide guidance in research related to hemozoin utilization.

Original languageEnglish
Article number1162
JournalPolymers
Volume11
Issue number7
DOIs
Publication statusPublished - 2019 Jul 1

Keywords

  • Biopolymers
  • Heme
  • Hemozoin
  • HRP-II
  • Polymerase

ASJC Scopus subject areas

  • Chemistry(all)
  • Polymers and Plastics

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