Enhanced performance of a surface plasmon resonance immunosensor for detecting Ab-GAD antibody based on the modified self-assembled monolayers

Hyung Choi Sue, Wook Lee Jung, Jun Sim Sang

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

To enhance the feasibility of surface plasmon resonance (SPR) immunosensor as a tool for diagnosing type I diabetes, we enhanced the sensitivity of immunoresponse for detecting the monoclonal anti-glutamic acid decarboxylase (GAD) antibody by modification of mixed self-assembled monolayers (SAMs). The effects of the different mixed SAMs were evaluated with respect to the degree of streptavidin immobilization, the degree of biotin-GAD immobilization, and the immunoresponse sensitivity. Consequently, the sensitivity of the immunoresponse for the detection of anti-GAD antibody was enhanced as a result of the reduction in steric hindrance brought about by using SAMs of heterogeneous lengths. The immunoresponse for detecting the monoclonal anti-GAD antibody was also enhanced with the reduction of the excess immobilization of biotin-GAD and the minimization of non-specific binding that resulted from the simple substitution of the spacer from a carboxylic-terminated SAM for the hydroxyl-terminated SAM.

Original languageEnglish
Pages (from-to)378-383
Number of pages6
JournalBiosensors and Bioelectronics
Volume21
Issue number2
DOIs
Publication statusPublished - 2005 Aug 15

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Keywords

  • Glutamic acid decarboxylase
  • Immunosensor
  • Mixed self-assembled monolayers
  • Streptavidin
  • Surface plasmon resonance

ASJC Scopus subject areas

  • Biotechnology
  • Biophysics
  • Biomedical Engineering
  • Electrochemistry

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