Enhanced performance of a surface plasmon resonance immunosensor for detecting Ab-GAD antibody based on the modified self-assembled monolayers

Hyung Choi Sue, Wook Lee Jung, Sang Jun Sim

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

To enhance the feasibility of surface plasmon resonance (SPR) immunosensor as a tool for diagnosing type I diabetes, we enhanced the sensitivity of immunoresponse for detecting the monoclonal anti-glutamic acid decarboxylase (GAD) antibody by modification of mixed self-assembled monolayers (SAMs). The effects of the different mixed SAMs were evaluated with respect to the degree of streptavidin immobilization, the degree of biotin-GAD immobilization, and the immunoresponse sensitivity. Consequently, the sensitivity of the immunoresponse for the detection of anti-GAD antibody was enhanced as a result of the reduction in steric hindrance brought about by using SAMs of heterogeneous lengths. The immunoresponse for detecting the monoclonal anti-GAD antibody was also enhanced with the reduction of the excess immobilization of biotin-GAD and the minimization of non-specific binding that resulted from the simple substitution of the spacer from a carboxylic-terminated SAM for the hydroxyl-terminated SAM.

Original languageEnglish
Pages (from-to)378-383
Number of pages6
JournalBiosensors and Bioelectronics
Volume21
Issue number2
DOIs
Publication statusPublished - 2005 Aug 15
Externally publishedYes

Fingerprint

Immunosensors
Glutamate Decarboxylase
Surface Plasmon Resonance
Surface plasmon resonance
Self assembled monolayers
Antibodies
Acids
Immobilization
Biotin
Streptavidin
Medical problems
Type 1 Diabetes Mellitus
Hydroxyl Radical
Substitution reactions

Keywords

  • Glutamic acid decarboxylase
  • Immunosensor
  • Mixed self-assembled monolayers
  • Streptavidin
  • Surface plasmon resonance

ASJC Scopus subject areas

  • Biotechnology
  • Analytical Chemistry
  • Electrochemistry

Cite this

Enhanced performance of a surface plasmon resonance immunosensor for detecting Ab-GAD antibody based on the modified self-assembled monolayers. / Sue, Hyung Choi; Jung, Wook Lee; Sim, Sang Jun.

In: Biosensors and Bioelectronics, Vol. 21, No. 2, 15.08.2005, p. 378-383.

Research output: Contribution to journalArticle

@article{799e84039b2f4b538b1bdd997af80369,
title = "Enhanced performance of a surface plasmon resonance immunosensor for detecting Ab-GAD antibody based on the modified self-assembled monolayers",
abstract = "To enhance the feasibility of surface plasmon resonance (SPR) immunosensor as a tool for diagnosing type I diabetes, we enhanced the sensitivity of immunoresponse for detecting the monoclonal anti-glutamic acid decarboxylase (GAD) antibody by modification of mixed self-assembled monolayers (SAMs). The effects of the different mixed SAMs were evaluated with respect to the degree of streptavidin immobilization, the degree of biotin-GAD immobilization, and the immunoresponse sensitivity. Consequently, the sensitivity of the immunoresponse for the detection of anti-GAD antibody was enhanced as a result of the reduction in steric hindrance brought about by using SAMs of heterogeneous lengths. The immunoresponse for detecting the monoclonal anti-GAD antibody was also enhanced with the reduction of the excess immobilization of biotin-GAD and the minimization of non-specific binding that resulted from the simple substitution of the spacer from a carboxylic-terminated SAM for the hydroxyl-terminated SAM.",
keywords = "Glutamic acid decarboxylase, Immunosensor, Mixed self-assembled monolayers, Streptavidin, Surface plasmon resonance",
author = "Sue, {Hyung Choi} and Jung, {Wook Lee} and Sim, {Sang Jun}",
year = "2005",
month = "8",
day = "15",
doi = "10.1016/j.bios.2004.07.037",
language = "English",
volume = "21",
pages = "378--383",
journal = "Biosensors",
issn = "0956-5663",
publisher = "Elsevier Limited",
number = "2",

}

TY - JOUR

T1 - Enhanced performance of a surface plasmon resonance immunosensor for detecting Ab-GAD antibody based on the modified self-assembled monolayers

AU - Sue, Hyung Choi

AU - Jung, Wook Lee

AU - Sim, Sang Jun

PY - 2005/8/15

Y1 - 2005/8/15

N2 - To enhance the feasibility of surface plasmon resonance (SPR) immunosensor as a tool for diagnosing type I diabetes, we enhanced the sensitivity of immunoresponse for detecting the monoclonal anti-glutamic acid decarboxylase (GAD) antibody by modification of mixed self-assembled monolayers (SAMs). The effects of the different mixed SAMs were evaluated with respect to the degree of streptavidin immobilization, the degree of biotin-GAD immobilization, and the immunoresponse sensitivity. Consequently, the sensitivity of the immunoresponse for the detection of anti-GAD antibody was enhanced as a result of the reduction in steric hindrance brought about by using SAMs of heterogeneous lengths. The immunoresponse for detecting the monoclonal anti-GAD antibody was also enhanced with the reduction of the excess immobilization of biotin-GAD and the minimization of non-specific binding that resulted from the simple substitution of the spacer from a carboxylic-terminated SAM for the hydroxyl-terminated SAM.

AB - To enhance the feasibility of surface plasmon resonance (SPR) immunosensor as a tool for diagnosing type I diabetes, we enhanced the sensitivity of immunoresponse for detecting the monoclonal anti-glutamic acid decarboxylase (GAD) antibody by modification of mixed self-assembled monolayers (SAMs). The effects of the different mixed SAMs were evaluated with respect to the degree of streptavidin immobilization, the degree of biotin-GAD immobilization, and the immunoresponse sensitivity. Consequently, the sensitivity of the immunoresponse for the detection of anti-GAD antibody was enhanced as a result of the reduction in steric hindrance brought about by using SAMs of heterogeneous lengths. The immunoresponse for detecting the monoclonal anti-GAD antibody was also enhanced with the reduction of the excess immobilization of biotin-GAD and the minimization of non-specific binding that resulted from the simple substitution of the spacer from a carboxylic-terminated SAM for the hydroxyl-terminated SAM.

KW - Glutamic acid decarboxylase

KW - Immunosensor

KW - Mixed self-assembled monolayers

KW - Streptavidin

KW - Surface plasmon resonance

UR - http://www.scopus.com/inward/record.url?scp=22044436139&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=22044436139&partnerID=8YFLogxK

U2 - 10.1016/j.bios.2004.07.037

DO - 10.1016/j.bios.2004.07.037

M3 - Article

VL - 21

SP - 378

EP - 383

JO - Biosensors

JF - Biosensors

SN - 0956-5663

IS - 2

ER -