Enhanced production of 1,2-propanediol by tpi1 deletion in Saccharomyces cerevisiae

Joon Young Jung, Eun Sil Choi, Min Kyu Oh

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

Saccharomyces cerevisiae was metabolically engineered to improve 1,2-propanediol production. Deletion of the tpi1 (triosephosphate isomerase) gene in S. cerevisiae increased the carbon flux to DHAP (dihydroxylacetone phosphate) in glycolysis, resulting in increased glycerol production. Then, the mgs and gldA genes, the products of which convert DHAP to 1,2-propanediol, were introduced to the tpi1-deficient strain using a multicopy plasmid. As expected, the intracellular level of methylglyoxal was increased by introduction of the mgs gene in S. cerevisiae and that of 1,2-propanediol by introduction of both the mgs and gldA genes. As a result, 1.11 g/l of 1,2-propanediol was achieved in flask culture.

Original languageEnglish
Pages (from-to)1797-1802
Number of pages6
JournalJournal of microbiology and biotechnology
Volume18
Issue number11
DOIs
Publication statusPublished - 2008 Nov 28

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Keywords

  • 1,2-propanediol
  • Metabolic engineering
  • Saccharomyces cerevisiae
  • Triosephosphate isomerase

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology

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