Enhanced production of cellobiose dehydrogenase and β-glucosidase by Phanerochaete chrysosporium

Eun Ji Kim, Han Suk Choi, Seong Woo Kang, Kwang Ho Song, Sung Ok Han, Chulhwan Park, Seung Wook Kim

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

The production of cellobiose dehydrogenase (CDH) and β-glucosidase by Phanerochaete chrysosporium ATCC 32629 was assessed during submerged fermentation. The maximum concentrations of CDH and β-glucosidase were obtained using rice straw as the carbon source. Organic nitrogen sources were more effective in enzyme production than inorganic nitrogen sources. Corn steep liquor (CSL) for CDH production and soy bean meal (SBM) for β-glucosidase production were the most appropriate organic nitrogen sources. Using optimum medium obtained by response surface methodology (RSM), the maximum concentrations of CDH and β-glucosidase achieved in the stirred-tank reactor (STR) were 204 U/L and 140 U/L, respectively. CDH productivity (22.7 U/L·day) was the highest at 9 days.

Original languageEnglish
Pages (from-to)77-81
Number of pages5
JournalKorean Journal of Chemical Engineering
Volume29
Issue number1
DOIs
Publication statusPublished - 2012 Jan

Keywords

  • Cellobiose Dehydrogenase
  • Phanerochaete chrysosporium
  • Response Surface Method
  • Rice Straw
  • β-Glucosidase

ASJC Scopus subject areas

  • Chemistry(all)
  • Chemical Engineering(all)

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