Enhanced production of enveloped viruses in BST-2-deficient cell lines

Eunbi Yi, Jinsoo Oh, Ngoc Q. Giao, Soohwan Oh, Se Ho Park

Research output: Contribution to journalArticlepeer-review

6 Citations (Scopus)

Abstract

Despite all the advantages that cell-cultured influenza vaccines have over egg-based influenza vaccines, the inferior productivity of cell-culture systems is a major drawback that must be addressed. BST-2 (tetherin) is a host restriction factor which inhibits budding-out of various enveloped viruses from infected host cells. We developed BST-2-deficient MDCK and Vero cell lines to increase influenza virus release in cell culture. BST-2 gene knock-out resulted in increased release of viral particles into the culture medium, by at least 2-fold and up to 50-fold compared to release from wild-type counterpart cells depending on cell line and virus type. The effect was not influenza virus/MDCK/Vero-specific, but was also present in a broad range of host cells and virus families; we observed similar results in murine, human, canine, and monkey cell lines with viruses including MHV-68 (Herpesviridae), influenza A virus (Orthomyxoviridae), porcine epidemic diarrhea virus (Coronaviridae), and vaccinia virus (Poxviridae). Our results suggest that the elimination of BST-2 expression in virus-producing cell lines can enhance the production of viral vaccines. Biotechnol. Bioeng.2017;114: 2289–2297.

Original languageEnglish
Pages (from-to)2289-2297
Number of pages9
JournalBiotechnology and Bioengineering
Volume114
Issue number10
DOIs
Publication statusPublished - 2017 Oct

Keywords

  • BST-2
  • MDCK
  • cell-cultured vaccine
  • influenza virus

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

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