Enhanced production of polyhydroxybutyrate from syngas by using nanoscaled cellulose particles with a syngas-converting enzyme complex immobilized on Ralstonia eutropha

Sang Kyu Shin, Young Jin Ko, Da Woon Jeong, Myeong Eun Lee, Seung Kyou You, Jeong Eun Hyeon, Sung Ok Han

Research output: Contribution to journalArticlepeer-review

4 Citations (Scopus)

Abstract

Synthetic gas (syngas) produced from pyrolysis or a blast furnace is a mixture of different ratio of H2, CO, N2, CH4, CO2 and other carbon-containing gas. CO2 and H2 are the key sources for Ralstonia eutropha, which uses CO2 to produce polyhydroxybutyrate (PHB) with a H2 uptake system. For the efficient conversion of syngas to accessible carbon source, nanoscaled cellulose particles with an enzyme complex consisting of carbon monoxide dehydrogenase (CooS), carbon monoxide binding unit (CooA) and carbonic anhydrase (CA) were assembled by minimized scaffoldin (mCbpA). In addition to this assembly, mCbpA-BspA (MB) established to bridge the nanoparticle assembly and R. eutropha was used to provide higher enzyme stability and activity. The highest PHB accumulation after 72 h of fermentation was 14.2 g/L in a closed serum bottle that contained the enzyme assembly immobilized on R. eutropha. This catalytic assembly may be a new potential conversion tool to produce biodegradable PHB from syngas.

Original languageEnglish
Article number124903
JournalJournal of Cleaner Production
Volume285
DOIs
Publication statusPublished - 2021 Feb 20

Keywords

  • Enzyme complex
  • Nanoscaled cellulose particles
  • Polyhydroxybutyrate
  • Ralstonia eutropha
  • Syngas utilization

ASJC Scopus subject areas

  • Renewable Energy, Sustainability and the Environment
  • Environmental Science(all)
  • Strategy and Management
  • Industrial and Manufacturing Engineering

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