Enhanced protection of pathogenic Escherichia coli ingested by a soil nematode Caenorhabditis elegans against sanitizer treatments

Mi Ri Park, Sangnam Oh, Hyun Sun Yun, Soon Han Kim, Young Ho Ko, Jee-Hoon Ryu, Min-Suk Rhee, Ok Shin, Younghoon Kim

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

We employed Caenorhabditis elegans as a model to study the effectiveness of sanitizers in killing pathogenic Escherichia coli strains ingested by free-living nematodes. Adult worms that had fed on six pathogenic E. coli strains (highly persistent in the nematode intestine) were treated with three chemical solutions. In planktonic cells, none of the H<inf>2</inf>O<inf>2</inf> and acetic acid treatments influenced the survival of the pathogenic E. coli strains, whereas sodium hypochlorite critically decreased the viability of the strains. Importantly, the survival of the E. coli strains was dramatically increased by persistence in the C. elegans gut under 0.1% sodium hypochlorite, and several strains could survive at a concentration of 0.5%. In addition, all pathogenic E. coli strains in the C. elegans gut survived on the lettuce for 5 days even though they were washed with 0.1% sodium hypochlorite. Taken together, our results indicate that pathogenic E. coli ingested by C. elegans may be protected against washing treatment with commercial sanitizers on raw food materials.

Original languageEnglish
Pages (from-to)1917-1922
Number of pages6
JournalBioscience, Biotechnology and Biochemistry
Volume78
Issue number11
DOIs
Publication statusPublished - 2014

Fingerprint

Caenorhabditis elegans
Escherichia coli
Soil
Soils
Sodium Hypochlorite
Lettuce
Washing
Acetic Acid
Intestines

Keywords

  • Caenorhabditis elegans
  • Pathogenic E. Coli
  • Persistence
  • Raw food materials
  • Sanitizer

ASJC Scopus subject areas

  • Biotechnology
  • Biochemistry
  • Molecular Biology
  • Applied Microbiology and Biotechnology
  • Analytical Chemistry
  • Organic Chemistry
  • Medicine(all)

Cite this

Enhanced protection of pathogenic Escherichia coli ingested by a soil nematode Caenorhabditis elegans against sanitizer treatments. / Park, Mi Ri; Oh, Sangnam; Yun, Hyun Sun; Kim, Soon Han; Ko, Young Ho; Ryu, Jee-Hoon; Rhee, Min-Suk; Shin, Ok; Kim, Younghoon.

In: Bioscience, Biotechnology and Biochemistry, Vol. 78, No. 11, 2014, p. 1917-1922.

Research output: Contribution to journalArticle

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