Enhanced solubility of heterologous proteins by fusion expression using stress-induced Escherichia coli protein, Tsf

Kyung Yeon Han, Jong Am Song, Keum Young Ahn, Jin Seung Park, Hyuk Seong Seo, Jeewon Lee

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Through two-dimensional electrophoresis, Escherichia coli proteome response to a protein denaturant, guanidine hydrochloride, was analyzed and elongation factor Ts (Tsf) detected as a stress-induced protein. Many host proteins aggregated, or their synthesis levels decreased significantly under conditions of protein denaturation as 34 out of 699 soluble proteins knocked out and 63 proteins decreased by over 2.5-fold. Interestingly, the expression level of Tsf increased 1.61-fold compared with a nonstress condition. Contrary to direct expression, various heterologous proteins were solubly expressed in E. coli when subjected to N-terminus fusions of Tsf. Owing most likely to an intrinsic high folding efficiency, Tsf seemed to play critical roles in sequestering interactive surfaces of heterologous proteins from nonspecific protein-protein interactions leading to formation of inclusion bodies. It has been also demonstrated that Tsf is effective in aiding the production of a biologically active bacterial cutinase, which could be of interest to biotechnology and commercial applications.

Original languageEnglish
Pages (from-to)132-138
Number of pages7
JournalFEMS Microbiology Letters
Volume274
Issue number1
DOIs
Publication statusPublished - 2007 Sep 1

Fingerprint

Escherichia coli Proteins
Solubility
Proteins
Escherichia coli
Protein Denaturation
Inclusion Bodies
Guanidine
Proteome
Biotechnology
Heat-Shock Proteins
Electrophoresis
Membrane Proteins

Keywords

  • Escherichia coli BL21 (DE3)
  • Proteome
  • Solubility enhancer
  • Stress response
  • Tsf

ASJC Scopus subject areas

  • Genetics
  • Molecular Biology
  • Applied Microbiology and Biotechnology
  • Microbiology

Cite this

Enhanced solubility of heterologous proteins by fusion expression using stress-induced Escherichia coli protein, Tsf. / Han, Kyung Yeon; Song, Jong Am; Ahn, Keum Young; Park, Jin Seung; Seo, Hyuk Seong; Lee, Jeewon.

In: FEMS Microbiology Letters, Vol. 274, No. 1, 01.09.2007, p. 132-138.

Research output: Contribution to journalArticle

Han, Kyung Yeon ; Song, Jong Am ; Ahn, Keum Young ; Park, Jin Seung ; Seo, Hyuk Seong ; Lee, Jeewon. / Enhanced solubility of heterologous proteins by fusion expression using stress-induced Escherichia coli protein, Tsf. In: FEMS Microbiology Letters. 2007 ; Vol. 274, No. 1. pp. 132-138.
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