Enhancement in the sensitivity of an immobilized cell-based soil biosensor for monitoring PAH toxicity

Suk Tai Chang, Hyun Joo Lee, Man Bock Gu

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

A soil biosensor was developed in our previous study using both a recombinant bioluminescent bacterium, GC2 (lac::luxCDABE), immobilized with transparent glass beads within an agar matrix and rhamnolipids, a biosurfactant employed to increase the bioavailability of phenanthrene from contaminated soil. To improve the sensitivity of this biosensor, glass beads of various sizes were tested. The results showed that the detection limit of the biosensor was lowered when smaller-sized glass beads were employed. Further tests showed that the use of glass beads with smaller diameters enhanced the sensitivity of the biosensor, allowing for a more conclusive study on the phenanthrene toxicity present within soils.

Original languageEnglish
Pages (from-to)272-276
Number of pages5
JournalSensors and Actuators, B: Chemical
Volume97
Issue number2-3
DOIs
Publication statusPublished - 2004 Feb 1

Keywords

  • Bioluminescent bacteria
  • Biosensor
  • Enhancement
  • PAH
  • Sensitivity

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Instrumentation
  • Condensed Matter Physics
  • Surfaces, Coatings and Films
  • Metals and Alloys
  • Electrical and Electronic Engineering
  • Materials Chemistry

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