Enhancement in the viability and biosensing activity of freeze-dried recombinant bioluminescent bacteria

Sue Hyung Choi, Man Bock Gu

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

The genetically-engineered Escherichia coli strain, DPD2540, which contains a fabA::luxCDABE fusion gene, gives a bioluminescent output when membrane fatty acid synthesis is needed. For more practical application of this strain in the field as biosensor, freeze-drying was adopted. A 12% sucrose solution with Luria-Bertani (LB) broth, as determined by the viability after freeze-drying, was found to be the most effective composition for lyophilization solution among various compositions tested. Rapid freezing with liquid nitrogen also gave the best viability after freeze-drying as compared to samples frozen at -70°C and -20°C. The biosensing activities of the cells showed a greater sensitivity when the cells from the exponential phase were freeze-dried. Finally, the optimum temperature for use of the freeze-dried cells in the biosensor field was determined.

Original languageEnglish
Pages (from-to)202-206
Number of pages5
JournalBiotechnology and Bioprocess Engineering
Volume5
Issue number3
Publication statusPublished - 2000 Dec 1
Externally publishedYes

Fingerprint

Freeze Drying
Bacteria
Drying
Biosensors
Biosensing Techniques
Cells
Sugar (sucrose)
Liquid nitrogen
Chemical analysis
Fatty acids
Freezing
Escherichia coli
Sucrose
Gene Fusion
Fusion reactions
Fatty Acids
Genes
Membranes
Nitrogen
Temperature

Keywords

  • Bioluminescence
  • Cryoprotectant
  • Optimum freezing and monitoring temperatures
  • Recombinant bioluminescent bacteria

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology
  • Biomedical Engineering

Cite this

Enhancement in the viability and biosensing activity of freeze-dried recombinant bioluminescent bacteria. / Choi, Sue Hyung; Gu, Man Bock.

In: Biotechnology and Bioprocess Engineering, Vol. 5, No. 3, 01.12.2000, p. 202-206.

Research output: Contribution to journalArticle

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