Enhancement in the viability and biosensing activity of freeze-dried recombinant bioluminescent bacteria

Sue Hyung Choi, Man Bock Gu

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

The genetically-engineered Escherichia coli strain, DPD2540, which contains a fabA::luxCDABE fusion gene, gives a bioluminescent output when membrane fatty acid synthesis is needed. For more practical application of this strain in the field as biosensor, freeze-drying was adopted. A 12% sucrose solution with Luria-Bertani (LB) broth, as determined by the viability after freeze-drying, was found to be the most effective composition for lyophilization solution among various compositions tested. Rapid freezing with liquid nitrogen also gave the best viability after freeze-drying as compared to samples frozen at -70°C and -20°C. The biosensing activities of the cells showed a greater sensitivity when the cells from the exponential phase were freeze-dried. Finally, the optimum temperature for use of the freeze-dried cells in the biosensor field was determined.

Original languageEnglish
Pages (from-to)202-206
Number of pages5
JournalBiotechnology and Bioprocess Engineering
Volume5
Issue number3
DOIs
Publication statusPublished - 2000
Externally publishedYes

Keywords

  • Bioluminescence
  • Cryoprotectant
  • Optimum freezing and monitoring temperatures
  • Recombinant bioluminescent bacteria

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology
  • Biomedical Engineering

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