Enhancement method of limit of frequency resolution using magnetic bead on the microcantilever

Ki Yong Choi, Duck Kyun Choi, Sang Myung Lee, Yoon Sik Lee, Ga Young Han, Dae Sung Yoon, Tae Song Kim

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

The sensitivity of immunoassays strongly depends on the binding affinity of the antibodies, which is also limited by a variety of other factors that are specific to the assay configuration, the type of label and instrument system used for detection. Magnetic beads are used in order to increase sensitivity in diagnosis system using the piezoelectric microcantilever. Use of magnetic beads is seemingly viable to insure the high sensitivity as well as the stable immunoassay. The ultra-sensitive magneto-mechanic detection of protein is accomplished by monitoring resonant frequency change of a cantilever functionalized with magnetic beads. The resonant frequency is surely shifted by mass and magnetic field effect. As a result, we practiced sandwich assay using CRP secondary antibody-coated magnetic bead on cantilever. Resonant frequency of the cantilever is changed by mass effect. When CRP secondary antibody-coated magnetic beads are attached to a CRP antigen, the changes in normalized resonant frequency shift δf/f1 were found to definitely increase about 30 times.

Original languageEnglish
Title of host publication2006 5th IEEE Conference on Sensors
Pages148-151
Number of pages4
DOIs
Publication statusPublished - 2006 Dec 1
Event2006 5th IEEE Conference on Sensors - Daegu, Korea, Republic of
Duration: 2006 Oct 222006 Oct 25

Publication series

NameProceedings of IEEE Sensors

Other

Other2006 5th IEEE Conference on Sensors
CountryKorea, Republic of
CityDaegu
Period06/10/2206/10/25

ASJC Scopus subject areas

  • Electrical and Electronic Engineering

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    Choi, K. Y., Choi, D. K., Lee, S. M., Lee, Y. S., Han, G. Y., Yoon, D. S., & Kim, T. S. (2006). Enhancement method of limit of frequency resolution using magnetic bead on the microcantilever. In 2006 5th IEEE Conference on Sensors (pp. 148-151). [4178578] (Proceedings of IEEE Sensors). https://doi.org/10.1109/ICSENS.2007.355740